High-Performance Liquid Chromatographic Determination of Glycyrrhizin and Glycyrrhetinic Acid in Biological Materials

Ichikawa, Tsutomu; Ishida, Shiro; Sakiya, Yoko; Akada, Yoshinobu

doi:10.1248/cpb.32.3734

Cited by 42 publications

(19 citation statements)

References 6 publications

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“…From a consideration of the above, there is clearly a need for a simple analytical method by which to determine 1 and 2 in plant roots, pharmaceutical formulations, confectionery products and plasma samples. Several papers report analytical methods for the determination of 1 and 2 in different matrices including commercial products and biological fluids (Hurst et al, 1983;Ichikawa et al, 1984;Collinge et al, 1985;Hermesse et al, 1986;De Groot et al, 1988;Spinks and Fenwick, 1990;Yamamura et al, 1991;Raggi et al, 1994a;Okamura et al, 2001). Such procedures are mostly based on reversed phase HPLC with UV (Hurst et al, 1983;De Groot et al, 1988;Raggi et al, 1994b;Collinge et al, 1985;Okamura et al, 2001) or fluorometric detection (Yamamura et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous HPLC analysis, with isocratic elution, of glycyrrhizin and glycyrrhetic acid in liquorice roots and confectionery products

et al. 2005

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Glycyrrhizin (1), the main active principle of Glycyrrhiza glabra (liquorice) roots, is extensively used in herbal medicines, in pharmaceutical preparations and confectionery products. A feasible and reliable method which allows the simultaneous analysis of 1 and its aglycone, 18beta-glycyrrhetic acid (2), by means of an isocratic HPLC procedure is described. The system uses a C8 column as the stationary phase, and a mixture of acetonitrile, methanol, water and glacial acetic acid as the mobile phase. Good linearity was found in the concentration ranges 1-50 and 0.05-2.50 microg/mL for 1 and 2, respectively. A simple and rapid sample pre-treatment, based on the extraction of the two analytes with a mixture of water and ethanol, was developed for the examination of liquorice confectionery products and root samples. The HPLC method was shown to be appropriate, in terms of precision and feasibility, for the quality control of the analytes in these matrices.

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Section: Introductionmentioning

confidence: 99%

Simultaneous HPLC analysis, with isocratic elution, of glycyrrhizin and glycyrrhetic acid in liquorice roots and confectionery products

et al. 2005

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“…Subsequently, this licorice-induced form of hypertension mediated by glycyrrhetinic acid has been described in several other reports (4-7). Methods for quantitative determination of glycyrrhetinic acid in human serum include thin layer chromatography (8), immunoassay techniques (9, 10), gas chromatography with mass spectrometry (11) and liquid chromatography (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23). Most Chromatographie procedures require time-consuming and tedious extraction steps for example enrichment, preconcentration and prepurification.…”

Section: Introductionmentioning

confidence: 99%

Determination of 18β-Glycyrrhetinic Acid in Human Serum Using the Fully Automated ALCA-System

Heilmann¹,

Heide²,

Schöneshöfer³

1997

Clinical Chemistry and Laboratory Medicine

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Summary:We report a method for the determination of ISß-glycyrrhetinic acid (glycyrrhetinic acid) in human serum using the ALCA-system. The technology of the ALCA-system is based on the principles of adsorptive and desorptive processes between liquid and solid phases. The assay is run fully automated and selective. Procedural losses throughout the analysis are negligible, thereby allowing for external calibration. The calibration curve is linear up to 10 mg/1 and concentrations as low as 10 ^ are detectable. CV is 2.5% for within-and 7.5% for between-assay precision at a level of 50 g/l and 1.2% for within-and 8.5% for between-assay precision at a level of 500 g/l. Specific and expensive reagents are not necessary and time-consuming manual operations are not involved. This assay can be selected from a wide spectrum of methods at any time. Thus, the present method is well-suited for drug monitoring purposes in the routine laboratory. In a pharmacokinetic study we measured serum levels of glycyrrhetinic acid in ten healthy young volunteers after ingestion of 500 mg glycyrrhetinic acid. Maximum levels of glycyrrhetinic acid were 6.3 mg/1 2 to 4 hours after ingestion. Twenty-four (24) hours after ingestion seven probands still had glycyrrhetinic acid levels above the detection limit with a mean level of 0.33 mg/1.

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“…Analytical Procedure Samples of 1 ml for plasma and urine were used for G and GA determination. After vigorous shaking with 5 ml of methanol for 20 min, the samples were centrifuged for 30 min at 2000 rpm (13). For the G analysis, to the methanol used was added an internal standard PPB (2.5 x 10-7 moles/l).…”

Section: Introductionmentioning

confidence: 99%

Interaction of licorice on glycyrrhizin pharmacokinetics.

Cantelli‐Forti

Maffei

Hrelia

et al. 1994

Environ Health Perspect

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The effects of components of aqueous licorice root extract (LE) on the pharmacokinetics of glycyrrhizin (G) and glycyrrhetic acid (GA) were investigated in rats and humans. The aim of this work was to define the role of pharmacokinetics in G toxicity. In the procedure, G and GA were detected in biological fluids by means of recently improved HPLC methods. Significantly lower G and GA plasma levels were found in rats and humans treated with LE compared to the levels obtained with those in which G alone was administered. The pharmacokinetic curves showed significant differences in the areas under the plasma-time curve (AUC), Cmax, and Tmax parameters. The data obtained from urine samples are in agreement with the above results and confirm a reduced bioavailability of G present in LE compared to pure G. This should be attributed to the interaction during intestinal absorption between the G constituent and the several components in LE. The modified bioavailability could explain the various clinical adverse effects resulting from the chronic oral administration of G alone as opposed to LE. -Environ Health Perspect 102(Suppl 9): 65-68 (1994)

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High-Performance Liquid Chromatographic Determination of Glycyrrhizin and Glycyrrhetinic Acid in Biological Materials

Cited by 42 publications

References 6 publications

Simultaneous HPLC analysis, with isocratic elution, of glycyrrhizin and glycyrrhetic acid in liquorice roots and confectionery products

Simultaneous HPLC analysis, with isocratic elution, of glycyrrhizin and glycyrrhetic acid in liquorice roots and confectionery products

Determination of 18β-Glycyrrhetinic Acid in Human Serum Using the Fully Automated ALCA-System

Interaction of licorice on glycyrrhizin pharmacokinetics.

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