High-performance liquid chromatographic determination of glibenclamide in human plasma and urine

Emilsson, H.; Sjöberg, Stefan; Svedner, M.; Christenson, I

doi:10.1016/s0378-4347(00)83445-3

Cited by 40 publications

(16 citation statements)

References 19 publications

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“…At different time intervals after treatment, blood was collected from orbital sinuses using heparinized capillaries. Plasma was separated by centrifugation (Eltek instruments, India) and analyzed by reverse phase high-performance liquid chromatography method (18).…”

Section: Pharmacokinetic Studiesmentioning

confidence: 99%

Microcapsules and Transdermal Patch: A Comparative Approach for Improved Delivery of Antidiabetic Drug

2009

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Abstract. Glibenclamide (GL)-loaded microcapsules (MC) and transdermal patches (TDP) were formulated and in vitro and in vivo parameters compared to find out the best route of drug administration. The formulation TDP1 having a drug-polymer ratio 1:1 showed comparatively higher GL release and better permeation across mice skin (p<0.05). From the comparative study, it was concluded that the transdermal system of GL produced better improvement compared to oral microcapsule administration (p<0.05). The transdermal system exhibited comparatively slow and continuous supply of GL at a desired rate to systemic circulation avoiding metabolism, which improved day-to-day glycemic control in diabetic subjects. Transdermal system of GL exhibited better control of hyperglycemia and prolonged plasma half-life by transdermal systems (9.6±1.2 h) in comparison with oral microcapsule (5.84±2.1 h), indicating that the drug, when administered by transdermal systems, will remain in the body for a longer period. From the glucose tolerance test, transdermal route effectively maintained the normoglycemic levels in contrast to the oral group (MC1), which produced remarkable hypoglycemia ranging from −12.6±2.1% to −18±2.3%. The significantly high (p<0.05) area under the curve values observed with transdermal system (1,346.2±92.3 ng ml −1 h −1 ) also indicate increased bioavailability of the drug from these systems compared to the oral route (829.8±76.4 ng ml −1 h −1 ).

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Section: Pharmacokinetic Studiesmentioning

confidence: 99%

Microcapsules and Transdermal Patch: A Comparative Approach for Improved Delivery of Antidiabetic Drug

2009

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“…The results were compared to those obtained by applying reported reference one [21,22] and where Student's T-test and F-ratio were used for comparison. Results are shown in Tables 7 and 8.…”

Section: Comparison Between the Proposed Methods And The Reference Methodsmentioning

confidence: 99%

Design and Validation of Stability Indicating Assay of Glibenclamide Using RP-HPLC Technique in Both Bulk, Pharmaceutical Formulations and Human Plasma

Sm¹,

Me²,

Mh³

2017

J Appl Pharm

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A simple and sensitive high performance liquid chromatography method was developed for determination of glibenclamide in presence of its impurities in pharmaceutical dosage form and human plasma. Instrumentation of the method was very simple and used the mixture of acetonitrile: water (60:40, v/v) as the mobile phase. Separation was carried out on a BDS. Hypersil C 8 (5 um, 250 × 4.6 mm) column. Effects of composition of mobile phase in addition to flow rate and detection wave length were studied. Calibration was obeyed in the range of 20-100 µg/ml of glibenclamide. The method was validated according to ICH parameters.

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“…Literature survey revealed HPLC, RP-HPLC, LC-MS, Spectrofluorimetric and simultaneous UV spectrophotometric methods are reported for the estimation of metformin hydrochloride [2][3][4][5], pioglitazone hydrochloride [6][7][8][9] and glibenclamide [10] alone or in combination with other anti-diabetic agents. So the present study aim to develop a simple, selective and precise RP-HPLC method for the simultaneous estimation of MET, PIO and GLI in bulk drug samples and in combined dosage formulation.…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Metformin Hydrochloride, Pioglitazone Hydrochloride and Glibenclamide in Bulk and Pharmaceutical Dosage Forms

S¹

2014

IOSRJPBS

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High-performance liquid chromatographic determination of glibenclamide in human plasma and urine

Cited by 40 publications

References 19 publications

Microcapsules and Transdermal Patch: A Comparative Approach for Improved Delivery of Antidiabetic Drug

Microcapsules and Transdermal Patch: A Comparative Approach for Improved Delivery of Antidiabetic Drug

Design and Validation of Stability Indicating Assay of Glibenclamide Using RP-HPLC Technique in Both Bulk, Pharmaceutical Formulations and Human Plasma

Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Metformin Hydrochloride, Pioglitazone Hydrochloride and Glibenclamide in Bulk and Pharmaceutical Dosage Forms

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