High-performance liquid chromatographic determination of zolpidem, a new sleep inducer, in biological fluids with fluorimetric detection

Guinebault, P.; Dubruc, C.; Hermann, P; Thenot, J. P.

doi:10.1016/s0378-4347(00)83462-3

Cited by 41 publications

(16 citation statements)

References 3 publications

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“…Zolpidem was quantitated in plasma using high-performance liquid chromatography, preceded by a single extraction with diethyl ether of 500 pi of the sample adjusted to pH 12 with a 0.25 M sodium hydroxide solution [9],…”

Section: Methodsmentioning

confidence: 99%

Nocturnal Profile of Growth Hormone Secretion during Sleep Induced by Zolpidem: A Double-Blind Study in Young Adults and Children

Colle¹,

Rosenzweig

Bianchetti

et al. 1991

Horm Res

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Overnight blood sampling for repeated growth hormone (GH) assays, regarded as the most physiological assessment of GH status, may induce some disturbances in patients’ sleep and then in the evaluation of GH secretion. We studied the influence of a hypnotic drug, zolpidem (10 mg), on nocturnal GH profiles (GH peak, time to first and maximum GH peak, area under the curve, mean integrated concentration) over two nights at a 7-day interval, in a double-blind cross-over design in a group of 12 young adult volunteers (27.9 ± 4.3 years), and in a group of 12 children (10.8 ± 2.3 years) with short stature, in a parallel double-blind study. Mean GH profiles showed no difference between zolpidem-treated subjects and placebo-treated controls, either in adults or in children. Although in these experimental conditions, sleep onset latency was significantly reduced with zolpidem in the adult volunteers, the mean time to first GH peak remained unchanged. Furthermore, GH profile did not relate with sleep duration, sleep onset latency or number of awakenings. A hypnotic drug, such as zolpidem, given at bedtime, is therefore devoided of effect on nocturnal GH profile and may be used in young children for overnight blood sampling when needed.

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Section: Methodsmentioning

confidence: 99%

Nocturnal Profile of Growth Hormone Secretion during Sleep Induced by Zolpidem: A Double-Blind Study in Young Adults and Children

Colle¹,

Rosenzweig

Bianchetti

et al. 1991

Horm Res

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show abstract

“…Plasma was then frozen (-20°C) until assay. A high performance liquid chromatography method was used for zolpidem (analytical detection limit = 1 ng ml-) (Guinebault et al, 1986); a gas-phase chromatography method, adapted for capillary column separation, was used for flunitrazepam determination (analytical detection limit = 0.5 ng ml-') (Da Silva & Bekersky, 1976 (Carskadon et al, 1986).…”

Section: Methodsmentioning

confidence: 99%

Daytime wakefulness following a bedtime oral dose of zolpidem 20 mg, flunitrazepam 2 mg and placebo.

Bensimon

Foret

Warot

et al. 1990

Brit J Clinical Pharma

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“…Psychometric tests were always performed in the same sequence, in the presence of the same examiner, each volunteer being his own control. ZOL was measured in plasma by high performance liquid chromatography (HPLC) using an original method (Guinebault et al 1986). CPZ was extracted from plasma by the method of Gupta (Gupta et al 1981) and analyzed by HPLC using ion pairing on a reverse phase column (Stevenson and Reid 1981).…”

Section: Methodsmentioning

confidence: 99%

Possible interactions between zolpidem, a new sleep inducer and chlorpromazine, a phenothiazine neuroleptic

et al. 1988

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The combined use of a hypnotic and a neuroleptic is a rather frequent situation, encountered especially in the psychiatric sphere. We therefore tested zolpidem and chlorpromazine in six healthy subjects by using a double-blind latin square design. All of them received single doses of 20 mg zolpidem (ZOL), 50 mg chlorpromazine (CPZ) and the combination of ZOL + CPZ. The medication was given as a single dose in the morning and each treatment being separated by a 1-week interval. Zolpidem produced moderate to severe sedation varying according to the subjects. Psychometric performances (manual dexterity, Stroop test), alertness and psychomotricity (visual analogue scales) were reduced up to 3 h after drug intake. Chlorpromazine alone did not have much effect. Combined administration of ZOL and CPZ was rather more effective than ZOL alone. The pharmacokinetics of ZOL or CPZ remained unchanged except for the elimination half-life of CPZ, which increased significantly when administered along with ZOL. No other pharmacodynamic or pharmacokinetic interaction between ZOL and CPZ was evident. The fact that the ZOL and CPZ combination accentuated the pharmacodynamical effects can be explained to result from the summation of each of their own pharmacological effect.

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High-performance liquid chromatographic determination of zolpidem, a new sleep inducer, in biological fluids with fluorimetric detection

Cited by 41 publications

References 3 publications

Nocturnal Profile of Growth Hormone Secretion during Sleep Induced by Zolpidem: A Double-Blind Study in Young Adults and Children

Nocturnal Profile of Growth Hormone Secretion during Sleep Induced by Zolpidem: A Double-Blind Study in Young Adults and Children

Daytime wakefulness following a bedtime oral dose of zolpidem 20 mg, flunitrazepam 2 mg and placebo.

Possible interactions between zolpidem, a new sleep inducer and chlorpromazine, a phenothiazine neuroleptic

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