High-performance liquid chromatographic determination of 2-hydroxypropyl-γ-cyclodextrin in different biological fluids based on cyclodextrin enhanced fluorescence

Interactions of zearalenone with native and chemically modified cyclodextrins and their potential utilization

“…Since the presence of different organic solvents results in the fluorescence enhancement of ZEA as well [24] and because of we did not find data regarding the direct comparison of fluorescence enhancement of ZEA by organic solvents vs. CDs, we tested the effectiveness of methanol and acetonitrile (the most commonly applied organic components of reversed-phase HPLC eluents) vs. 2 mM BCD and DIMEB in ammonium acetate buffer (pH 5.0). Our results indicate that the application of methanol and/or acetonitrile can strongly enhance the fluorescence signal of ZEA and in this way increase the sensitivity of its fluorescence detection.…”

Rapid quantification of 2-hydroxypropyl-β-cyclodextrin in liquid pharmaceutical formulations by 1H nuclear magnetic resonance spectroscopy

“…Given that 2-HP-b-CD carbohydrates contain no chromophores, and that the molar mass is an average mass related to the average substitution degree, classical LC-UV analytical techniques are not applicable for their dosage. More specific approaches were therefore described for the quantification of CDs by using evaporative light scattering detection, mass spectrometry, refractive index detection, pulsed amperometry, colorimetric detection or based on cyclodextrin fluorescence enhancement (Agueros et al, 2005;Hammes et al, 2000;Hui Jiang et al, 2014;Szeman et al, 2002), but to the best of our knowledge, none of them was developed for the quantification of 2-HP-b-CD directly into commercialized liquid pharmaceutical formulations without any extraction or separation steps. Such technique is essential for a rapid and efficient quality control of CDs concentration in formulations when they are used as excipient and more importantly as active ingredient.…”

2D-Cosy NMR Spectroscopy as a Quantitative Tool in Biological Matrix: Application to Cyclodextrins

“…2D-NMR presents several advantages including its ability to specifically detect a substance of interest in biological matrices without any sample pre-treatments (except the addition of D 2 O and TMSP) or sample extractions which decrease the potential errors due to experimenter manipulations encounter with traditional sample treatments. Moreover, NMR seems to be particularly adapted to the analysis of hardly detectable pharmaceutical compounds such as cyclodextrins and poloxamers for which limited quantification techniques are available and generally required long sample pre-treatments including extraction or proteins precipitation (13,14). Taking into account these advantages, we decided to address this possible utilization of 2D-COSY spectroscopy for the identification and quantification of cyclodextrins in plasma medium.…”