High-performance liquid chromatographic determination of rutaecarpine in rat plasma: application to a pharmacokinetic study

Ko, Han Chieh; Tsai, Tung Hu; Chou, Cheng‐Jen; Hsu, Shin Yuan; Li, Shyh Yuan; Chen, Chieh Fu

doi:10.1016/s0378-4347(94)80128-2

Cited by 23 publications

(16 citation statements)

References 8 publications

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“…However, prolongation of haemostatic bleeding time by aspirin lasted longer than that of rutaecarpine in the mesenteric arteries of rats (Fig 3). This may be due, at least partly, to the half-life (t 1/2 ) of rutaecarpine being shorter than that of aspirin in vivo (Ko et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Antithrombotic effect of rutaecarpine, an alkaloid isolated from Evodia rutaecarpa, on platelet plug formation in in vivo experiments

Sheu

Hung

et al. 2000

Br J Haematol

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Summary. In this study, platelet thrombi formation was induced by irradiation of mesenteric venules with filtered light in mice pretreated intravenously with fluorescein sodium. Rutaecarpine (200 mg/g) significantly prolonged the latent period of inducing platelet plug formation in mesenteric venules when it was intravenously injected. Rutaecarpine (200 mg/g) prolonged occlusion time by approximately 1´5-fold (control 127^29 vs. taecarpine 188^23 s). Furthermore, aspirin (250 mg/g) also showed a similar prolongation of the occlusion time in this experiment. On a molar basis, rutaecarpine was approximately twofold more potent than aspirin at prolonging the occlusion time. Furthermore, rutaecarpine was also effective in reducing the mortality of ADP-induced acute pulmonary thromboembolism in mice when administered intravenously at doses of 25 and 50 mg/g. Intravenous injection of rutaecarpine (50 mg/g) significantly prolonged the bleeding time by approximately 1´5-fold compared with normal saline in the severed mesenteric arteries of rats. Continuous infusion of rutaecarpine (5 mg/g/min) also significantly increased the bleeding time 1´5-fold, and the bleeding time returned to baseline within 60 min after cessation of rutaecarpine infusion. These results suggest that rutaecarpine has an effective anti-platelet effect in vivo and that it may be a potential therapeutic agent for arterial thrombosis, but it must be assessed further for toxicity.

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Section: Discussionmentioning

confidence: 99%

Antithrombotic effect of rutaecarpine, an alkaloid isolated from Evodia rutaecarpa, on platelet plug formation in in vivo experiments

Sheu

Hung

et al. 2000

Br J Haematol

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“…Rutaecarpine is soluble in ethanol, benzene, chloroform, ether and acetonitrile (Chuang et al, 1996;Sheu, 1999;Ko et al, 1994). Ko et al (2002) used methanol to extract rutaecarpine from herbal medicine but also used acetonitrile to dissolve rutaecarpine (Ko et al, 1994). For the low water solubility of rutaecarpine, we used ethanol, methanol, acetonitrile or ethyl acetate for the extractive solvent from rat feces.…”

Section: Feces and Urine Sample Preparationmentioning

confidence: 99%

“…However, these published methods are generally tedious and time-consuming, or need mass spectrometer, which is expensive and not readily available in most clinical research laboratories. Ko et al (1994) method for determination of plasma rutaecarpine using a C 18 column with an acetonitrile-waterorthophosphoric acid (85%; 60:40:0.1, v/v/v, pH 2.5-2.8) as the mobile phase. The retention time of rutaecarpine was about 7 min.…”

Section: Measurement Of Rutaecarpine In Feces and Urine Samplementioning

confidence: 99%

“…Several high-performance liquid chromatography (HPLC) methods have been used to examine the concentration of rutaecarpine in plasma or in decoctions of Chinese medicine (Ko et al, 1994(Ko et al, , 2002Ueda et al, 1996). In addition, Ueda et al (1996) developed an ion-paired HPLC method to evaluate the contents of rutaecarpine in oriental pharmaceutical decoctions containing Evodia fruit.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, Ueda et al (1996) developed an ion-paired HPLC method to evaluate the contents of rutaecarpine in oriental pharmaceutical decoctions containing Evodia fruit. A reversed-phase HPLC was used to determine rutaecarpine concentration in rat plasma (Ko et al, 1994) and in decoction (Ko et al, 2002). However, there have been no reports evaluating the fecal and urinary excretion of rutaecarpine.…”

Section: Introductionmentioning

confidence: 99%

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Elimination of rutaecarpine and its metabolites in rat feces and urine measured by liquid chromatography

Jan

Lin

Don

et al. 2006

Biomed. Chromatogr.

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Rutaecarpine is an alkaloid isolated from the medicinal herb Evodia rutaecarpa. This study was to evaluate the elimination pathway of rutaecarpine in rat feces and urine. Rutaecarpine and its metabolites (3-, 10-, 11- and 12-hydroxyrutaecarpine) in urine were measured after incubation with beta-glucuronidase. After the rutaecarpine was administered (25 and 100 mg/kg) orally to rats, the urine and fecal samples were collected using a metabolic cage for five consecutive days. For determining rutaecarpine, the mobile phase consisted of acetontrile-10 mM NaH(2)PO(4) (60:40, v/v, pH 4.2 adjusted with orthophosphoric acid) with a flow rate of 1 mL/min. The calibration curve was linear in concentrations of 0.05-50 microg/mL in fecal and urine sample. The results indicated that more than 42% of the rutaecarpine was excreted by feces after oral administration (25 and 100 mg/kg), but only a small amount of rutaecarpine was detected in urine at a higher dose of rutaecarpine (100 mg/kg). After incubation with beta-glucuronidase, the hydroxyrutaecarpine in urine was eluted using methanol-acetonitrile-0.04% formic acid (6:30:64, v/v) with a flow rate of 1.2 mL/min. We conclude that the metabolic pathway of rutaecarpine went through phase I hydroxylation and phase II conjugation, and the major metabolite is 10-hydroxyrutaecarpine eliminated from urine of the rat.

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Pharmacokinetic Characterization of Dehydroevodiamine in the Rat Brain

Ahn

Jeon

Tsuruo

et al. 2004

Journal of Pharmaceutical Sciences

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High-performance liquid chromatographic determination of rutaecarpine in rat plasma: application to a pharmacokinetic study

Cited by 23 publications

References 8 publications

Antithrombotic effect of rutaecarpine, an alkaloid isolated from Evodia rutaecarpa, on platelet plug formation in in vivo experiments

Antithrombotic effect of rutaecarpine, an alkaloid isolated from Evodia rutaecarpa, on platelet plug formation in in vivo experiments

Elimination of rutaecarpine and its metabolites in rat feces and urine measured by liquid chromatography

Pharmacokinetic Characterization of Dehydroevodiamine in the Rat Brain

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