High-Performance Liquid Chromatographic Determination of Components of Bleomycin Preparations

A sensitive fluorescence turn-on assay of bleomycin and nuclease using WS2 nanosheet as an effective sensing platform

“…3B). The detection limit was estimated to be 0.3 nM from three times the standard deviation corresponding to the blank sample detection, which is much lower than those of traditional HPLC [8] or RIA [9]. We also compared the BLM assay performance (including assay time, detection limit and linear detection range) with that of other recently reported methods (Table S1).…”

“…Although BLM possesses the advantages of low myelosuppression and low immunosuppression, it also exhibits some serious dose-limiting side effects which are potential for pulmonary fibrosis and pneumonitis [6,7]. Thus, to achieve the best therapeutic effect, some methods have been developed for quantitatively monitoring the level of BLM, such as highperformance liquid chromatography (HPLC) [8], radioimmunoassay (RIA) [9], enzyme immunoassay (EIA) [10], electrochemical detection [11], fluorescent analysis [12,13] and colorimetric assay [14][15][16]. Among these establish methods, using BLMÁFe(II) complex-induced selective degradation of DNA as a signal transduction is especially attractive in recent years [11][12][13].…”

Stability of an extemporaneously prepared bleomycin–lidocaine–epinephrine intradermal admixture used in dermatology

“…Chromatographic conditions were adapted with some modifications, from the European Pharmacopoeia bleomycin monograph. 17,19 Separation was performed at room temperature on a reverse phase C18 (ODS) column (Chromolith Õ Performance RP-18 e, 100 mm  4.6 mm id, Merck) preceded by a C18 (ODS) guard column (Chromolith Õ RP-18e, 5  4.6 mm id, Merck). In order to obtain a good resolution between bleomycin peaks and a shorter analysis run time (30 min per injection), sequential gradient elution, at a flow rate of 1.2 mL/min, was used with a mobile phase composed of phase A: methanol and phase B: sodium 1-heptanesulfonate 5 mM (1.01 g/L) and disodium edetate (1.86 g/L) dissolved in aqueous solution of acetic acid (4.8 g/L).…”

Electrochemical determination of bleomycins based on dual-amplification of 4-mercaptophenyl boronic acid-capped gold nanoparticles and dopamine-capped gold nanoparticles

“…In order to sensitively detect BLMs, many methods have been developed over the past decades, such as high-performance liquid chromatography (HPLC), [18][19][20][21][22] radioimmunoassay (RIA), 17,23,24 enzyme immunoassay (EIA), 25,26 microbiological assay, 27,28 and uorescence resonance energy transfer (FRET). 29 However, the above methods were usually challenged with some disadvantages, such as complicated instruments for HPLC, high cost for RIA and EIA, and tedious experimental procedures for FRET.…”