High-performance liquid chromatographic determination of certain salicylates and their major metabolites in plasma following topical administration of a liniment to healthy subjects

The Arabidopsis thaliana Dihydroxyacetone Phosphate Reductase Gene SUPPRESSOR OF FATTY ACID DESATURASE DEFICIENCY1 Is Required for Glycerolipid Metabolism and for the Activation of Systemic Acquired Resistance[W]

“…SA was extracted with 5 mL of a mixture containing cyclohexane:ethylacetate:isopropanol (50:50:1). The sample was dried under N 2 gas and dissolved in 0.5 mL of the mobile phase (69:27:4 mix of water:methanol:glacial acetic acid) (Dadgar et al, 1985). Samples were filtered through a 0.22-mm filter, and 20 to 100 mL were used for high performance liquid chromatography.…”

Simultaneous determination of gentisic, salicyluric and salicylic acid in human plasma using solid-phase extraction, liquid chromatography and electrospray ionization mass spectrometry

“…AcidRP-HPLC-MS/SIM RP-HPLC-FLD lower limit of detection (LOD) and the limit of quantitation (LOQ) for GA, SUA and SA by applying ESI-MS detection was in the same order of magnitude that obtained by fluorescence detection: LOD between 5 12. and 7.12 ng/ml, LOQ between 16.23 and 20.87 ng/ml for GA, SUA and SA.…”

“…The Table 3 Concentration of gentisic (GA), salicyluric (SUA) and salicylic acid (SA) in a pure plasma sample obtained by mass spectrometric (MS) and fluorescence detection (FLD) applying calibration with aqueous standards or spiked plasma Table 3 shows their concentrations obtained as mean of six consecutive determinations, which also depend on the detection method (MS and FLD) used. Compared to earlier published analytical procedures for the analysis of GA, SUA and SA in biological fluids, this new method provides a 100 times increase in sensitivity instead of using LLE [11,12] and a 20 times increase instead of using earlier described SPE procedures [17]. The concentrations achieved with the calibration established upon the injection of spiked plasma samples and the two different detection methods does not differ much except for SA, which can be explained by the stronger tailing of this substance using FLD, caused by interference with coeluting plasma components.…”

“…The most common technique for the analysis of Aspirin ® and its major metabolites in plasma extracts is reversed-phase high-performance liquid chromatography (RP-LC), using either UV-absorbance or fluorescence detection (FLD) [8][9][10]. Traditionally, liquid-liquid extraction (LLE) is the preferred method for the separation of salicin and its salicylate derivatives from human plasma samples [11][12][13]; e.g., Klimés et al [14] described a method of LLE with methylene chloride for the isolation of acetylsalicylic acid, its metabolites, salicylic, gentisic and possibly salicyluric acid from whole blood, isolated erythrocytes and plasma. Separation was performed using reversed-phase chromatography on Separon SGX C18 by applying a mixture of methanol-water (80:100, v/v) as the mobile phase that allowed the determination down to a limit of 20 and 50 ng/ml for SA and GA, respectively.…”

Salicylate Ion-Selective Electrode Based on New Tetranuclear Copper Complexes of O-Vannlin-methionine as Neutral Carriers

“…So scientists have reported many methods for determinination of salicylic ion in the literature, varying from fluorometry, 2 chromatography, [3][4][5] and spectrophotometry 6,7 to volammetric procedures. Most of the methods are tedious and time-consuming.…”