High-performance liquid chromatographic determination of PEG 600 in human urine

Analytical Measurement of PEGylated Molecules

“…HPLC analysis of clinical samples usually requires pretreatment steps to reduce sample complexity. Typically, proteins are first removed by precipitation with chloroform, , acetonitrile, 5-sulfosalicylic acid, trifluoroacetic acid, ZnSO 4 /acetone, or ZnSO 4 –methanol . Acetonitrile and chloroform are preferred precipitation agents because nonvolatile salts can precipitate in analytical columns.…”

“…The limit of detection can be improved by modification of the PEG chain. For example, attachment of a dibenzoate group at the terminal end of PEG600 allowed UV detection at 230 nm, thereby greatly improving the limit of detection to 1 μg per injection . However, the hydrophobic properties of the benzoate group may alter the pharmacokinetic behavior of PEG, limiting the general utility of this method.…”

Periodate Oxidation of Peg–600, an Essential Pharmaceutical Polymer

“…Among other PEGs, PEG-600 is widely used to understand the intestinal physiology in human beings as it has low toxicity and freely excreted along with urine [8]. It is also extensively useful in the preparation of ointment bases and as a food additive.…”

Improved separation of polyethylene glycols widely differing in molecular weight range by reversed-phase high performance liquid chromatography and evaporative light scattering detection

“…Their use as so-called non-ionic surfactants and emulsifiers for pharmaceuticals [1][2][3][4][5][6][7][8][9][10][11] has been described and, owing to their low toxicity, they also play an important role in studies of intestinal permeability and adsorption in man [12][13][14]. Furthermore, when covalently coupled to proteins and enzymes, extensive changes in protein pharmacology, immunogenicity and enzymatic action [151 are observed.…”