High-performance liquid chromatographic determination of tramadol and its O-desmethylated metabolite in blood plasma

Nobilis, Milan; Kopeckỳ, J.; Kvĕtina, J; Chládek, Jaroslav; Svoboda, Zbyněk; Voříšek, Viktor; Perlı́k, F; Pour, Milan; Kuneš, Jiřı́

doi:10.1016/s0021-9673(01)01567-9

Cited by 67 publications

(45 citation statements)

References 26 publications

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“…A number of assays have been reported for detection of TR in biological and pharmaceutical samples, including chromatographic [5][6][7][8][9][10][11] , mass spectrometric [11][12][13] , spectrophotometric [14][15][16] ,and Capillary electrophoresis 17 . However, chromatography requires the use of organic solvents, expensive instruments, and time-consuming operating steps [5][6][7][8][9][10] . The reported method of spectrophotometry was based upon the oxidation reaction between the drug and alkaline potassium permanganate,or was based on the reaction of tramadol hydrochloride with 4-chloro-7-nitrobenzofurazan (NBD-Cl) 13 .…”

Section: Introductionmentioning

confidence: 99%

Investigated the Supramolecular Interaction of Tramadol Hydrochloride With P-Sulfonated Calix[4,6,8]arene

Zhao

Chen

Pang

et al. 2015

J. Chil. Chem. Soc.

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In this paper, the interaction behavior of p-sulfonated calix[n]arene (SCnA) with tramadol hydrochloride was investigated by fluorescence spectra, 1 H NMR spectra, and theoretical calculations in aqueous solution. At the optimized conditions, the fluorescence intensity of tramadol hydrochloride showed positive correlation to the concentration of SCnA, which led to a validated, simple, and sensitive fluorescence quenching method for the determination of tramadol hydrochloride was established for the first time. Moreover, the interaction based on SCnA superstructure provided has promising potential for therapeutic monitoring and pharmacokinetics and clinical application

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Section: Introductionmentioning

confidence: 99%

Investigated the Supramolecular Interaction of Tramadol Hydrochloride With P-Sulfonated Calix[4,6,8]arene

Zhao

Chen

Pang

et al. 2015

J. Chil. Chem. Soc.

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“…On the other hand, wastewater treatment plants (WWTPs) are unable to completely remove pharmaceutical compounds, and thus their effluents are also sources of these compounds in aquatic systems [2][3][4]. Therefore, residues (i.e., unchanged compounds and their metabolites) are continuously discharged into surface waters, where they can cause toxic effects Different analytical techniques have been reported for the simultaneous quantification of tramadol and its metabolites in biological samples, such as the brain tissue of mice and rats [22], saliva [23], urine [23,24], amniotic fluid [25], plasma [23,[26][27][28][29][30][31][32][33][34][35][36][37][38][39], and environmental samples [11,40], but reports regarding enantiomeric quantification are scarce.…”

Section: Introductionmentioning

confidence: 99%

Enantiomeric Separation of Tramadol and Its Metabolites: Method Validation and Application to Environmental Samples

et al. 2017

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Abstract:The accurate assessment of racemic pharmaceuticals requires enantioselective analytical methods. This study presents the development and validation of an enantioselective liquid chromatography with a fluorescence detection method for the concomitant quantification of the enantiomers of tramadol and their metabolites, N-desmethyltramadol and O-desmethyltramadol, in wastewater samples. Optimized conditions were achieved using a Lux Cellulose-4 column 150 × 4.6 mm, 3 µm isocratic elution, and 0.1% diethylamine in hexane and ethanol (96:4, v/v) at 0.7 mL min −1 . The samples were extracted using 150 mg Oasis ® mixed-mode cation exchange (MCX) cartridges. The method was validated using a synthetic effluent of a laboratory-scale aerobic granular sludge sequencing batch reactor. The method demonstrated to be selective, accurate, and linear (r 2 > 0.99) over the range of 56 ng L −1 to 392 ng L −1 . The detection and the quantification limits of each enantiomer were 8 ng L −1 and 28 ng L −1 for tramadol and N-desmethyltramadol, and 20 ng L −1 and 56 ng L −1 for O-desmethyltramadol. The feasibility of the method was demonstrated in a screening study in influent and effluent samples from a wastewater treatment plant. The results demonstrated the occurrence of tramadol enantiomers up to 325.1 ng L −1 and 357.9 ng L −1 , in the effluent and influent samples, respectively. Both metabolites were detected in influents and effluents.

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“…The spectrofluorimetry [16], HPLC with DAD detection [17], HPLC with UV detection [18][19][20], HPLC with fluorescence detection [21][22][23][24], LC/PDA-ESI-MS/MS [24], potentiometry [25,26], square-wave voltammetric method with amperometric detection [27], gas chromatography with mass spectrophotometer [28,29], gas chromatography with nitrogen-phosphorus detection [30], HPTLC [31][32][33] methods reported for the determination of TDL are sensitive enough but require expensive or sophisticated instruments, involve procedures with rigorous control of the experimental conditions, require long and tedious pretreatment of the samples and laborious clean up procedures prior to analysis and are not simple for routine analysis. However, spectrophotometric methods are particularly attractive because of ease in accessibility and their quick applicability to routine analysis.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Assay of Opioid Analgesic, Tramadol, Using Horseradish Peroxidase

N¹,

Srilakshmi

Alekya³

et al. 2014

ILNS

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Three novel and sensitive enzymatic methods have been developed for the quantification of tramadol in pharmaceutical dosage forms. The proposed methods are based on the reaction of tramadol with 3-methylbenzothiazoline-2-one hydrazone (method A), aniline (method B) and aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase to give colored complexes. The colored complexes obtained with 3-methylbenzothiazoline-2-one hydrazone, aniline and aminoantipyrine exhibit absorption maxima at 480 nm, 550 nm and 530 nm, respectively. Regression analysis of Beer's plots showed good correlation for tramadol in the concentration range (μg/mL) 2-12 for the methods A & B and 4-24 for the method C. The experimental parameters were studied and optimized. The precision and accuracy of the methods were satisfactory. The proposed methods were successfully applied for the quantification of tramadol in 3 brands of commercially available tablet dosage forms. The results were compared satisfactorily with the official method.

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High-performance liquid chromatographic determination of tramadol and its O-desmethylated metabolite in blood plasma

Cited by 67 publications

References 26 publications

Investigated the Supramolecular Interaction of Tramadol Hydrochloride With P-Sulfonated Calix[4,6,8]arene

Investigated the Supramolecular Interaction of Tramadol Hydrochloride With P-Sulfonated Calix[4,6,8]arene

Enantiomeric Separation of Tramadol and Its Metabolites: Method Validation and Application to Environmental Samples

Enzymatic Assay of Opioid Analgesic, Tramadol, Using Horseradish Peroxidase

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