High-performance liquid chromatographic determination of quercetin in human plasma and urine utilizing solid-phase extraction and ultraviolet detection

Ishii, Kazuo; Furuta, Takahisa; Kasuya, Yasuji

doi:10.1016/s1570-0232(03)00398-2

Cited by 110 publications

(47 citation statements)

References 24 publications

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“…Several methods were proposed in the literature to determine quercetin in the samples of apple and tomato juice and fruits, wines, teas, serums and pharmaceutical preparations. These include HPLC [13][14][15], HPTLC [16] LC-MS [17], spectroscopic methods [5,18,19], adsorptive stripping voltammetry [20], electrochemical analysis [21] and fluorimetric methods [22]. , CAS number 6151-25-3, Fluka AG, Buchs, Germany), acetonitrile (J.T.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms

Pavun

Đurđević

Jelikić-Stankov

et al. 2014

Maced. J. Chem. Chem. Eng.

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A simple, accurate and precise method based on the fluorescence properties of the aluminum(III)-quercetin complex, for the determination of quercetin, has been developed and validated. The complex has strong emission at pH 3.30,  em = 480 nm, with  ex = 420 nm. The linearity range of quercetin determination was 1.5-60.5 ng ml -1 with LOD 0.09 ng ml -1 and LOQ 0.27 ng ml -1. Recovery values in the range of 99.9-100.2% indicate a good accuracy of the method. The established method was applied for the determination of quercetin in capsules, with a recovery value of 98.3%, standard deviation of 0.22% and coefficient of variation of 0.09%.The reliability of the method was checked by the newly developed RP-HPLC/UV method for capsules with the direct determination of quercetin after separation. The good agreement between the two methods indicates the applicability of the proposed spectrofluorimetric method for quercetin determination in pharmaceutical dosage forms, with high reproducibility, and enables the direct and simple determination without its prior extraction from samples.The proposed spectrofluorimetric method has much better sensitivity and LOD and LOQ values that are about 1000 times lower than data reported in the literature.Keywords: quercetin; flavonoids; spectrofluorimetric determination; RP-HPLC; capsules СПЕКТРОФЛУОРОМЕТРИСКО ОПРЕДЕЛУВАЊЕ НА КВЕРЦЕТИН ВО ФАРМАЦЕВТСКИ ДОЗИРАНИ ФОРМИРазвиен и валидиран е едноставен, точен и прецизен метод за определување на кверцетин заснован на флуоресцентните својства на комплексот кверцетин-алуминиум(III). Комплексот покажува силна емисија при pH 3,30,  em = 480 nm и  ex = 420 nm. Линеарниот опсег за определување на кверцетин е 1,5-60,5 ng ml -1 со LOD 0,09 ng ml -1 и LOQ 0,27 ng ml -1. Вредностите на аналитичкиот принос од 99,9-100,2% покажуваат добра точност на методот. Воспоставениот метод е применет за определување на кверцетин во капсули со вредност на аналитичкиот принос од 98.3%, стандардна девијација од 0,22% и коефициент на варијација од 0,09%.Веродостојноста на методот е проверена со новововедениот RP-HPLC/UV метод за капсули со директно определување на кверцетин по негова сепарација. Доброто согласување на двата метода укажува на применливост на предложениот спектрофлуорометриски метод за опреде-лување на кверцетин во фармацевтски дозирани форми со висока репродуцибилност и овозможува директно и едноставно определување без претходна екстракција од примероците.Предложениот спектрофлуорометриски метод има многу подобра осетливост и вредностите LOD и LOQ се за околу 1000 пати пониски од оние дадени во литературата.

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Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms

Pavun

Đurđević

Jelikić-Stankov

et al. 2014

Maced. J. Chem. Chem. Eng.

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“…Several methods were proposed in the literature to determine QRC in the samples of apple and tomato juice and fruits, wines, teas, serums and pharmaceutical preparations. These include HPLC [8][9][10], HPTLC [11] LC-MS [12], spectroscopic methods [13,14], adsorptive stripping voltammetry [15], electro-chemical analysis [16] and fluorimetric methods [17]. The majority of methods required some pre-treatment of real samples, such as solid phase extraction [18] and molecularly imprinted polymers (MIPs).…”

Section: Introductionmentioning

confidence: 99%

Normal Flow and Stopped Flow Injection Spectrophotometric Determination of Quercetin Dihydrate Dietary Supplements

2017

IJSR

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Simple and sensitive normal flow injection(nFI) and stopped-FI spectrophotometric methods for the determination of quercetin dehydrate (QRC) in pure form were proposed, The methods were based on diazotization and coupling reaction between QRC and diazotized metoclopramide in alkaline medium to form orange-water soluble dye. The dye is stable and has a maximum absorbance at 438nm. A calibration graph shows that a Beers law is obeyed over the concentration range of 1-60µg.ml -1 and 0.8-50µg.ml -1 for (QRC) with a detection limit is 0.4166 µg.ml

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“…It has been reported that iridoid glycosides and flavonoids are two major components of L. rotata [3][4][5]. Many HPLC [6][7][8][9], CE [10][11][12][13][14], highperformance liquid chromatographic-mass spectrometric (HPLC-MS) [15][16][17], and gas chromatographic-mass spectrometric (GC-MS) [18,19] methods have been developed for the separation and analysis of iridoid glycosides and flavonoids. 8-O-acetylshanzhiside methylester, 6-O-acetylshanzhiside methylester, shanzhiside methylester, 8-deoxyshanzhiside, sesamoside, loganin, penstemoside, phlomiol, 7, 8-dehydropenstemoside, phloyoside I, phloyoside II, lamiophlomiside, phlorigidoside C, and seven other iridoid diglycosides have been isolated and reported as iridoid glycosides in the last 20 years [20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Separation and analysis of phenylethanoid glycosides inLamiophlomis rotataby high-performance liquid chromatography

et al. 2013

Acta Chromatographica

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Summary.A simple hydrolysis method has been developed for determination of phenylethanoid glycosides in Lamiophlomis rotata (L.R.). Different kinds of phenylethanoid glycosides were hydrolyzed in hydrochloric acid solution to produce corresponding phenethyl alcohols and cinnamic acids, mainly containing hydroxytyrosol, homovanillyl alcohol, 3,4-dimethoxyphenethyl alcohol, caffeic acid, fumalic acid and 3,4-dimethoxycinnamic acid. The six analytes could be determined simultaneously by high-performance liquid chromatography (HPLC). The effects of mobile phase, pH and concentration of running buffer, detection wavelength, flow rate and injection volume were also investigated. Under the optimum conditions, the six hydrolyzates could be perfectly separated within 45 min. The response was linear over four orders of magnitude with detection limits (S/N ＝ 3) ranging from 1 × 10 −8 to 1.5 × 10 −4 mol L −1 for the analytes. The method has been successfully applied to the analysis of real sample Du-Yi-Wei capsule and Qi-Zheng-Yan-Tong patch, with satisfactory results.

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High-performance liquid chromatographic determination of quercetin in human plasma and urine utilizing solid-phase extraction and ultraviolet detection

Cited by 110 publications

References 24 publications

Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms

Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms

Normal Flow and Stopped Flow Injection Spectrophotometric Determination of Quercetin Dihydrate Dietary Supplements

Separation and analysis of phenylethanoid glycosides inLamiophlomis rotataby high-performance liquid chromatography

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