High-performance liquid chromatographic investigations on some enzymes of papaya latex

Calam, D.H.; Davidson, J.; Harris, Roy

doi:10.1016/s0021-9673(01)87435-5

Cited by 10 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main constituents of papaya latex are papain and chymopapain (Calam et al 1985). Devi & Singh (1978) reported marked oedema and haemorrhagic placentas in fetuses of the rats given oral and intra-peritoneal doses of papain.…”

Section: Discussionmentioning

confidence: 99%

Papaya (<I>Carica papaya</I>) consumption is unsafe in pregnancy: fact or fable? Scientific evaluation of a common belief in some parts of Asia using a rat model

Adebiyi

Adaikan²,

Prasad

2002

British Journal of Nutrition

View full text Add to dashboard Cite

Using controlled in vivo and in vitro pharmacological methods, we evaluated the safety of papaya (Carica papaya ) consumption in pregnancy with reference to its common avoidance during pregnancy in some parts of Asia. Ripe papaya (Carica papaya L. (Caricaecae) blend (500 ml/l water) was freely given to four groups of Sprague-Dawley rats at different stages of gestation (days 1 -5, 6 -11, 12-17 and 1 -20). The control group received water. The effect of ripe papaya juice and crude papaya latex on pregnant and non-pregnant rats' uteri was also evaluated using standard isolated-organ-bath methods. The daily volumes (ml) of ripe papaya blend consumed by the treated group were significantly (P,0·05) more than water consumed by the control (control 40·3 (SD 11·6) v. treated 64 (SD 19·0)). There was no significant difference in the number of implantation sites and viable fetuses in the rats given ripe papaya relative to the control. No sign of fetal or maternal toxicity was observed in all the groups. In the in vitro study, ripe papaya juice (0·1-0·8 ml) did not show any significant contractile effect on uterine smooth muscles isolated from pregnant and non-pregnant rats; conversely, crude papaya latex (0·1-3·2 mg/ml) induced spasmodic contraction of the uterine muscles similar to oxytocin (1-64 mU/ml) and prostaglandin F 2a (0·028 -1·81 mM). The response of the isolated rat uterine smooth muscles to 0·2 mg crude papaya latex/ml was comparable to 0·23 mM prostaglandin F 2a and 32 mU oxytocin/ml. In the 18 -19 d pregnant rat uterus, the contractile effect of crude papaya latex was characterized by tetanic spasms. The results of the present study suggest that normal consumption of ripe papaya during pregnancy may not pose any significant danger. However, the unripe or semi-ripe papaya (which contains high concentration of the latex that produces marked uterine contractions) could be unsafe in pregnancy. Though evaluation of potentially toxic agents often relies on animal experimental results to predict risk in man, further studies will be necessary to ascertain the ultimate risk of unripe papaya-semi-ripe papaya consumption during pregnancy in man.

show abstract

Section: Discussionmentioning

confidence: 99%

Papaya (<I>Carica papaya</I>) consumption is unsafe in pregnancy: fact or fable? Scientific evaluation of a common belief in some parts of Asia using a rat model

Adebiyi

Adaikan²,

Prasad

2002

British Journal of Nutrition

View full text Add to dashboard Cite

show abstract

“…The samples were prepared exactly as described hyCalam('/f//. [ 18] and analysed byreversedphase HPLC on a Beck man Ultrasphere ODS column as described by Noyes [19].…”

Section: Production Of a Dog Hair/dander Extractmentioning

confidence: 99%

The allergens of dog. I. Identification using crossed radio‐immunoelectrophoresis

Ford

Alterman

Kemeny

1989

Clin Experimental Allergy

View full text Add to dashboard Cite

The antigens present in an extract of dog hair and dander were examined by crossed immunoelectrophoresis (CIE) and the IgE-binding allergens by crossed radio-immunoelectrophoresis (CRIE), respectively, using sera from 60 British and Finnish animal-allergic subjects. The extract was comprised of a minimum of 28 antigens, 11 of which were common to dog serum. IgE antibody in the sera of the dog-sensitive patients bound to 21 of the 28 antigens at varying frequencies and intensities. Binding of any intensity occurred most frequently to two serum proteins: antigen 23 (IgG) binding IgE in 88% of cases, and antigen 3 (dog serum albumin, DSA) in 77% of cases. Dander antigen 8 bound in 63% and antigen 1 in 42% of the sera. Strong IgE binding, however, was most commonly associated with dander antigen 8 followed by antigens 1 and 23 (IgG) then 3 (DSA). The ranking of the antigens as allergens was similar for the two populations except that DSA was more important for the British than for the Finnish subjects.

show abstract

Purification technologies for plant proteins

Jervis

Pierpoint

1989

Journal of Biotechnology

View full text Add to dashboard Cite

High-performance liquid chromatographic investigations on some enzymes of papaya latex

Cited by 10 publications

References 10 publications

Papaya (<I>Carica papaya</I>) consumption is unsafe in pregnancy: fact or fable? Scientific evaluation of a common belief in some parts of Asia using a rat model

Papaya (<I>Carica papaya</I>) consumption is unsafe in pregnancy: fact or fable? Scientific evaluation of a common belief in some parts of Asia using a rat model

The allergens of dog. I. Identification using crossed radio‐immunoelectrophoresis

Purification technologies for plant proteins

Contact Info

Product

Resources

About