High-performance liquid chromatographic mapping of the oligosaccharides released from the humanised immunoglobulin, CAMPATH ™1H

Lines, Anne C.

doi:10.1016/0731-7085(95)01646-5

Cited by 12 publications

(3 citation statements)

References 13 publications

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“…1), and various truncated structures with the loss of the core fucose, GlcNAc, or both. 48,63,[113][114][115][116][117][118][119][120][121][122][123][124][125][126][127] Oligosaccharides of high mannose have also been reported for several antibodies. 33,62,97,103,111,[128][129][130][131] Sialic acid is normally present at a very low level for Fc oligosaccharides.…”

Section: Chemical Modifications Disulfide Bondsmentioning

confidence: 99%

Heterogeneity of Monoclonal Antibodies

Liu

Gaza-Bulseco

Faldu

et al. 2008

Journal of Pharmaceutical Sciences

424

343

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Section: Chemical Modifications Disulfide Bondsmentioning

confidence: 99%

Heterogeneity of Monoclonal Antibodies

Liu

Gaza-Bulseco

Faldu

et al. 2008

Journal of Pharmaceutical Sciences

424

343

View full text Add to dashboard Cite

“…In previous studies, – a phosphate buffer consisting of acetonitrile was often used to separate the PMP−sugars. The volume ratios of acetonitrile to phosphate buffer and the pH level of the mobile phase, which had great effects on the retention and separation of the PMP derivatives of d -quinovose and other sugars in S. japonicus , are discussed here.…”

Section: Resultsmentioning

confidence: 99%

“…The n -butanol extracted solids contained triterpene glycosides equal to only 2.7% of the dried organism content, and more than 90% of the other composition was eliminated. Fucose was one of the major monosaccharides of S. japonicus polysaccharide, – which would be the primary influence for the determination of PMP−Qui; therefore, the peak area ratios of PMP−Fuc/PMP−Qui shown in Table would represent the elimination of the influence of the compositions in the sample matrix. The results ( Table ) indicated that the PMP−Fuc/PMP−Qui ratio values were significantly reduced after either pretreatment procedure.…”

Section: Resultsmentioning

confidence: 99%

Determination of Triterpene Glycosides in Sea Cucumber (Stichopus japonicus) and Its Related Products by High-Performance Liquid Chromatography

Dong

Xue

et al. 2008

J. Agric. Food Chem.

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A creative and sensitive method has been developed for the determination of triterpene glycosides concentrations in sea cucumber ( Stichopus japonicus) and related products by using d-quinovose (6-deoxyglucose) as the measurement standard by reverse-phase high-performance liquid chromatography (HPLC) and variable-wavelength detection. d-quinovose, which is a unique monosaccharide in holostane triterpene glycosides, was liberated by acid hydrolysis and precolumn derivatized by 1-phenyl-3-methyl-5-pyrazolone (PMP). PMP-quinovose was analyzed by HPLC with 22% acetonitrile in 0.05 M KH2PO4 aquatic solution (pH 5.2) as mobile phase. The calibration curves of d-quinovose were linear within the range of 6.56-164 mg/L (r(2) > 0.995). The contents of triterpene glycosides in various S. japonicus products were determined after appropriate pretreatment methods. The concentration of triterpene glycosides was calculated by the formula C = C(qui) x alpha (alpha = 8.5). The result showed that this method was a simple, rapid, and stable method for the determination of triterpene glycosides in S. japonicus products.

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A Sensitive Derivatization Method for the Determination of the Sugar Composition after Pre‐column Reductive Amination with 3‐Amino‐9‐ethylcarbazole (AEC) by High‐Performance Liquid Chromatography

2007

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3-Amino-9-ethylcarbazole (AEC) was employed for monosaccharide derivatization. The derivatives can be analyzed by high-performance liquid chromatography (HPLC) with an ultraviolet detection (wavelength: 254 nm). Monosaccharides were quantitatively derivatized with 3-amino-9-ethylcarbazole (AEC) at 70 for 60 min. The ℃ method was linear for all samples over the concentration range tested (r＞0.999), the precision was found to be satisfactory (R.S.D.＜3%), and the recovery ratios were ＞98.62%. The stability analysis showed R.S.D. was between 1.81%-3.16%. Detection limits for the samples (D-glucose, L-xylose, D-mannose, L-arabinose, and L-rhamnose) ranged from 0.06 to 1.97 ng/mL (S/N＝3). Under the optimized derivatization and HPLC conditions, five monosaccharides were well separated using a narrow bore C18 column (250 mm×4.6 mm) with 0.1 mol/L ammonium acetate containing 25% acetonitrile at a flow rate of 0.5 mL/min. As an application, the method has been successfully applied to the determination of monosaccharide compositions of three polysaccharides SPPA-1, SPPB-1 and SPPC-1 of Spirulina platensis. This method also has potential application to oligosaccharide or glycan analyses.

show abstract

High-performance liquid chromatographic mapping of the oligosaccharides released from the humanised immunoglobulin, CAMPATH ™1H

Cited by 12 publications

References 13 publications

Heterogeneity of Monoclonal Antibodies

Heterogeneity of Monoclonal Antibodies

Determination of Triterpene Glycosides in Sea Cucumber (Stichopus japonicus) and Its Related Products by High-Performance Liquid Chromatography

A Sensitive Derivatization Method for the Determination of the Sugar Composition after Pre‐column Reductive Amination with 3‐Amino‐9‐ethylcarbazole (AEC) by High‐Performance Liquid Chromatography

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