High Performance Liquid Chromatographic Method for Prostaglandin E²Determination in Human Gastric Juice Without Derivatization

Abstract

A rapid and practical method for the separation and quantitation of PGE2 in human gastric juice by high performance liquid chromatography is described. Separation on a reversedphase column and W detection allows quantities as little as 20 nanograms of PGE2 to be detected. Specificity, sensitivity, high yield and reproducibility make this method particularly suitable for prostaglandin determination in human gastric juice.Recently, high performance liquid chromatography (HPLC) was introduced to separate PGs (1,3,6,7) which were then quantified by RIA (2,3) OK GC/MS (5,8).Derivatization of PGs has also been carried out f o r fluorescence and UV detection by HPLC (9,10,11), but derivative proce-Copyright 0 1984 by Marcel Dekker, Inc. 2409 01 48-391 91841071 2-2409$3.50/0