High-performance liquid chromatographic method for the determination of diosgenin in plants

Mahato, Shashi B.; Sahu, Niranjan P.; Roy, S. K.

doi:10.1016/s0021-9673(00)82621-7

Cited by 11 publications

(5 citation statements)

References 16 publications

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“…HPLC quantification of diosgenin: Plant samples were processed and analyzed for diosgenin according to Mahato et al [6]. Reverse phase HPLC (Perkin-Elmer) was performed with petroleum ether (b. p. 60 ± 80 8C) and isopropyl alcohol (12 : 1) as mobile phase, at a flow rate of 1 mL/min, pressure 500 psi (Binary LC pump 250).…”

Section: Methodsmentioning

confidence: 99%

In VitroMicropropagation, Differentiation of Aerial Bulbils and Tubers and Diosgenin Content inDioscorea bulbifera

Narula

Kumar²,

Bansal³

et al. 2003

Planta med

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Dioscorea bulbifera could be micropropagated through nodal segments and bulbils. The best medium for regeneration and bulbil differentiation was MS + 0.5 microM IAA (indole-3-acetic acid) + 20.0 microM Kn (kinetin) + 500 mg/L CH (casein hydrolysate) + activated charcoal (20 %). Diosgenin content was maximum in regenerants grown on MS + 5.0 microM IAA + 20.0 microM Kn + 500 mg/L CH. T.s of bulbils could also be used for direct plantlet differentiation as well as bulbil differentiation on MS + 10.0 microM IAA + 20.0 microM Kn + (in mg/L) 30 each of Asp (asparagine) + Arg (arginine) + Gln (glutamine) + 10 Ad (adenine) + 500 CH + 10 Cyst hyd (cysteine hydrochloride). Diosgenin yield in plantlets reached a maximum after 20 weeks. The results indicate that micropropagation, bulbil formation and tuberisation can be achieved in vitro in D. bulbifera, hitherto a less exploited plant, and can further be used for obtaining enhanced levels of diosgenin.

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Section: Methodsmentioning

confidence: 99%

In VitroMicropropagation, Differentiation of Aerial Bulbils and Tubers and Diosgenin Content inDioscorea bulbifera

Narula

Kumar²,

Bansal³

et al. 2003

Planta med

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“…It is assumed that these factors caused the difference in the amounts of furostanol and spirostanol glycosides in Ja-I yam and Yang-Ming-Shan yam. Chen and Wu (13) and D. prazeri, respectively (23) . However, the contents of saponins in the glycoside forms, e.g., furostanol and spirostanol glycosides in yams have not been reported yet.…”

Section: Determination Of Steroidal Saponins In Yammentioning

confidence: 99%

Simultaneous determination of furostanol and spirostanol glycosides in Taiwanese yam (Dioscorea spp.) cultivars by high performance liquid chromatography

Yang¹,

Lu²,

Hwang³

2020

Journal of Food and Drug Analysis

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A high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of the steroidal saponins, furostanol and spirostanol glycosides, in Taiwanese yam cultivars. C18 column kept at 45 was used as the stationary phase, the mobile phase was a binary solvent system consisting of methanol and deionized water (62/38, v/v) in the first 20 min and 71/29 (v/v) from 21 to 65 min at a flow rate of 1 ml/min. Both evaporative light scattering detector (ELSD) and UV (203 to 215 nm) were employed and compared for the detection of saponins. The detection limits for ELSD and 203 nm were 2~3.5 µg and 0.1~0.2 µg, respectively at signal-to-noise ratio of 10. The recoveries of furostanol and spirostanol glycosides in the range of 0.25~3 mg were all above 92%. The presence of steroidal saponins in Taiwanese yams was also determined.

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“…Several reports are available in literature with the report of diosgenin quantification in different species of Dioscorea as 0.2% in D. polygonoides 35 , 0.2-2.3% in D. althaeoides 36 , 1.92% in D. prazeri 37 , 1.3% in D. villosa 38 , 0.18-0.55% D. zingiberenzis 39 and 0.02-2.64% in D. polygonoid 40 . Here for the first time we are reporting the diosgenin quantification in tissue cultured microtubers of D. floribunda.…”

Section: Augmentation Of Diosgeninmentioning

confidence: 99%

In vitro Diosgenin Augmentation in Microtubers of Dioscorea floribunda (M. Martens & Galeotti)

Yadav¹,

Sangwan²,

Odimegwu³

et al. 2017

TJNPR

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Medicinal yam Dioscorea floribunda is known to possess pharmacologically active diosgenin, a steroidal sapogenin, usually found in greater quantities in the tubers. It is used for the commercial synthesis of cortisone, pregnenolone, progesterone, and other steroid products. Diosgenin is also implicated in reduction of serum cholesterol, estrogenic activity and anticancer effects.In the present study, multiple shoots were initiated from nodal explants and microtuberization, from budding nodal segments of D. floribunda using Murashige and Skoog medium supplemented with varying quantities and combinations of plant hormones; benzyl amino purine, indole-3butyric acid, naphthalene acetic acid and kinetin. There was a differential response in the medium consisting of hormones and their concentrations on diosgenin accumulation in tubers. The best performance was observed in media supplemented with 2 mg L −1 naphthalene acetic acid and 0.5 mg L −1 kinetin producing viable tubers only in 3 months of culture period and accumulating the higher amount of diosgenin content. Also, results reveal that the faster multiple shoot formation and microtuberization is achieved more efficiently in liquid media. Thus, we have achieved diosgenin production and augmentation in microtubers of D. floribunda.

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High-performance liquid chromatographic method for the determination of diosgenin in plants

Cited by 11 publications

References 16 publications

In VitroMicropropagation, Differentiation of Aerial Bulbils and Tubers and Diosgenin Content inDioscorea bulbifera

In VitroMicropropagation, Differentiation of Aerial Bulbils and Tubers and Diosgenin Content inDioscorea bulbifera

Simultaneous determination of furostanol and spirostanol glycosides in Taiwanese yam (Dioscorea spp.) cultivars by high performance liquid chromatography

In vitro Diosgenin Augmentation in Microtubers of Dioscorea floribunda (M. Martens & Galeotti)

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