High-performance liquid chromatographic method for the determination of the dinitrocarbanilide component of nicarbazin in eggs with on-line clean-up

The effect of cooking on veterinary drug residues in food: Nicarbazin (Dinitrocarbanilide component)

“…The extraction/clean-up procedure was based on the previously reported procedure developed by this laboratory (Tarbin et al 1993). For egg, extracts were produced by a crude homogenization in acetonitrile.…”

“…Samples from each were removed at measured time intervals from zero to 3 h for analysis. Samples were subject to extraction and clean-up by our standard method used for tissue samples (except taking 0.5 g oil) prior to determination (Tarbin et al 1993).…”

Liquid chromatographic tandem mass spectrometric determination of five coccidiostats in poultry eggs and feed

“…Other techniques used were LC with UV detection [40][41][42][43][44][45][46][47], gas-liquid chromatography [48] and capillary isotachophoresis combined with capillary zone electrophoresis [49]. Most papers describing a method for the detection of nicarbazin in different matrices, use LC with UV detection [50][51][52][53][54][55][56][57][58][59][60][61][62][63][64]. Also papers describing LC-MS [4,5,59,[65][66][67][68][69], electrochemical methods [70,71] and pulse polarographic methods [70,72,73] are available.…”

Efficient HPLC method for the determination of nicarbazin, as dinitrocarbanilide in broiler liver

“…A number of residue methods have been developed for the determination of DNC in eggs [8][9][10][11][12][13][14][15], muscle [14][15][16][17] and liver [12][13][14][15][16][17]. Published methods for determination of DNC in liver are LC-MS [11,13,14,16], HPLC [8][9][10]15,17] and Biacore TM biosensor [12] based methods. The available HPLC methods involve liquid-liquid partitioning using large volumes of organic solvent [8,15] or are MSPD (matrix solid phase dispersion) based and lack sensitivity [17].…”