High performance liquid chromatographic profiling of tryptophan and related indoles in body fluids and tissues of carcinoid patients

Kema, IP; Schellings, Amj; Hoppenbrouwers, Cjm; Rutgers, H.M.; Vries, Elisabeth G.E. de; Muskiet, Faj

doi:10.1016/0009-8981(93)90029-4

Cited by 78 publications

(58 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are no published reports about cyclic changes in serotonin metabolism in carcinoid patients, although Pietraszek et al (19 ) reported fluctuations in the concentrations of serotonin and its metabolites in blood during the day in healthy controls and depressed patients. In healthy adults, we found no clear diurnal variations in urinary 5-HIAA excretion based on two 12-h urine samples (20 ). In line with our results, Singh et al Although symptoms of the carcinoid syndrome seem to be exacerbated by physical exertion, food intake, or starting of the day, information about this subject is scarce.…”

Section: Discussionsupporting

confidence: 89%

Daily Cyclic Changes in the Urinary Excretion of 5-Hydroxyindoleacetic Acid in Patients with Carcinoid Tumors

Zuetenhorst¹,

Korse²,

Bonfrèr³

et al. 2004

Clinical Chemistry

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 89%

Daily Cyclic Changes in the Urinary Excretion of 5-Hydroxyindoleacetic Acid in Patients with Carcinoid Tumors

Zuetenhorst¹,

Korse²,

Bonfrèr³

et al. 2004

Clinical Chemistry

View full text Add to dashboard Cite

“…The brains were rapidly frozen in liquid nitrogen and kept at Ϫ80°C. After homogenization of the whole brains, 5-HT, 5-hydroxyindoleacetic acid (5-HIAA), and tryptophan (Trp) amounts were measured with high-pressure liquid chromatography (HPLC) and fluorometric detection (Kema et al, 1993).…”

Section: Biochemical Analysismentioning

confidence: 99%

Effects of monoamine oxidase A inhibition on barrel formation in the mouse somatosensory cortex: Determination of a sensitive developmental period

et al. 1998

View full text Add to dashboard Cite

Genetic inactivation of monoamine oxidase A (MAOA) in C3H/HeJ mice causes a complete absence of barrels in the somatosensory cortex, and similar alterations are caused by pharmacological inhibition of MAOA in wild type mice. To determine when and how MAOA inhibition affects the development of the barrel field, the MAOA inhibitor clorgyline was administered to mice of the outbred strain OF1 for various time periods between embryonic day 15 (E15) and postnatal day 7 (P7), and the barrel fields were analyzed with cytochrome oxidase and Nissl stains in P10 and adult mice. High-pressure liquid chromatography measures of brain serotonin (5-HT) showed three- to eightfold increases during the periods of clorgyline administration. Perinatal mortality was increased and weight gain was slowed between P3 and P6. Clorgyline treatments from E15 to P7 or from P0 to P7 disrupted the formation of barrels in the anterior snout representation and in parts of the posteromedial barrel subfield (PMBSF). Treatments from P0 to P4 caused similar although less severe barrel field alterations. Clorgyline treatments only during embryonic life or starting on P4 caused no detectable abnormalities. In cases with barrel field alterations, a rostral-to-caudal gradient of changes was noted: Rostral barrels of the PMBSF were most frequently fused and displayed an increased size tangentially. Thus, MAOA inhibition resulting in increased brain levels of 5-HT affects barrel development during the entire first postnatal week, with a sensitive period between P0 and P4. The rostral-to-caudal gradient of changes in the barrel field parallels known developmental gradients in the sensory periphery and in the maturation thalamocortical afferents. The observed barrel fusions could correspond to a default in the initial segregation of thalamic fibers or to a continued, exuberant growth of these fibers that overrides the tangential domain that is normally devoted to individual whiskers.

show abstract

“…The URL for platelet serotonin was 5.4 nmol serotonin/10 9 platelets. An extended description of the sampling procedures and analytical methods has been published (18 ). immunohistochemistry Paraffin-embedded sections (4 m), mounted on 3-aminopropyltriethoxysilane (Sigma-Aldrich Chemie) were deparaffinized according to standard procedures.…”

Section: Markers Of Serotonin Metabolismmentioning

confidence: 99%

Catecholamine-Synthesizing Enzymes in Carcinoid Tumors and Pheochromocytomas

Meijer¹,

Copray²,

Hollema

et al. 2003

Clinical Chemistry

View full text Add to dashboard Cite

Background: Serotonin is the principal endocrine product of carcinoid tumors, but simultaneously increased production of catecholamines has been described in these tumors. As it is not clear whether these tumors contain specific enzymes for catecholamine synthesis, we aimed to detect catecholamine-synthesizing enzymes [tyrosine hydroxylase (TH), dopamine-␤-hydroxylase (DBH), and phenylethanolamine-N-methyltransferase (PNMT)] in midgut carcinoid tumors and pheochromocytoma and to correlate enzyme expression to serotonin production as well as catecholamines and metabolites excreted in urine. Methods: Paraffin-embedded tumor specimens from 21 midgut carcinoid patients and 20 pheochromocytoma patients (10 sporadic and 10 MEN type IIa-related tumors) were stained for TH, DBH, and PNMT, using a three-step biotin-avidin-peroxidase method. Results: TH was demonstrated in 9 (43%) of 21 carcinoids and in all (100%) of 20 pheochromocytomas, DBH in 8 (38%) carcinoids and in 15 (75%) pheochromocytomas, and PNMT in 7 (33%) carcinoids and in 13 (65%) pheochromocytomas. Increased urinary excretion of catecholamines and metabolites was observed in 10 (48%) carcinoid patients and in all pheochromocytoma patients. No clinically relevant association between enzyme expression and urinary excretion of catecholamines and metabolites was found. Conclusions: Catecholamine-synthesizing enzymes are present in many carcinoid tumors. This finding possibly indicates the existence of a catecholamine-synthesizing

show abstract

High performance liquid chromatographic profiling of tryptophan and related indoles in body fluids and tissues of carcinoid patients

Cited by 78 publications

References 41 publications

Daily Cyclic Changes in the Urinary Excretion of 5-Hydroxyindoleacetic Acid in Patients with Carcinoid Tumors

Daily Cyclic Changes in the Urinary Excretion of 5-Hydroxyindoleacetic Acid in Patients with Carcinoid Tumors

Effects of monoamine oxidase A inhibition on barrel formation in the mouse somatosensory cortex: Determination of a sensitive developmental period

Catecholamine-Synthesizing Enzymes in Carcinoid Tumors and Pheochromocytomas

Contact Info

Product

Resources

About