High-performance liquid chromatographic separation of the enantiomers of organophosphorus pesticides on polysaccharide chiral stationary phases

Ellington, J. Jackson; Evans, John J.; Prickett, Keith B.; Champion, William L.

doi:10.1016/s0021-9673(01)01138-4

Cited by 88 publications

(6 citation statements)

References 15 publications

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“…The Q192R polymorphism affects the hydrolysis levels of some racemic OPs substrates (98,110,115,147). In our laboratory, the stereoselective hydrolysis of fenamiphos and HDCP by different PON1 Q192R alloforms from human serum has been characterized by chiral chromatographic methods (32,37). The stereoselective hydrolysis has been quantified by measuring the residual concentration (µM) of each insecticide enantiomer, after a specified incubation time with 2.5 mM calcium or 5 mM EDTA at physiological pH and temperature.…”

Section: Stereoselective Hydrolysis Of Commercial Op Pesticides By Alloforms Of Pon1 Q192rmentioning

confidence: 99%

Hydrolysis of chiral organophosphorus compounds by phosphotriesterases and mammalian paraoxonase-1

2021

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Section: Stereoselective Hydrolysis Of Commercial Op Pesticides By Alloforms Of Pon1 Q192rmentioning

confidence: 99%

Hydrolysis of chiral organophosphorus compounds by phosphotriesterases and mammalian paraoxonase-1

2021

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“…This allowed using many other solvents, such as chloroform, dichloromethane, and tetrahydrofuran, as mobile phases of HPLC. The application of these solvents, in many cases, not only increased the efficiency of separating enantiomers but also allowed for obtaining a different selectivity profile for some racemic mixtures [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Application of Polysaccharide‐Based Columns for the Separation of Racemic Mixtures Obtained Based on (R,S)‐2‐Octanol by Reversed‐Phase Chiral Liquid Chromatography

Urbańska

2024

Preprint

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The polysaccharide-based chiral stationary phase (CSP) columns for high-performance liquid chromatography (HPLC) were applied in the separation of liquid crystalline racemic mixtures obtained based on (R,S)-2-octanol and were found to be highly effective in recognizing isomers of minor structural differences. The mobile phase (MP), which consists of acetonitrile (ACN)/water (H2O) at different volume ratios, was used. All measurements were made at room temperature. The order of the elution was also determined. The chromatographic parameters such as resolution - (Rs), selectivity - (α), and the number of theoretical plates, i.e., column efficiency - (N), were determined. The polysaccharide-based CSP columns have unique advantages in separation technology, and this study has shown the potential usefulness of the CSP columns in separating liquid crystalline racemic mixtures belonging to the same homologous series.

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“…This allowed the use of many other solvents, such as chloroform, dichloromethane, and tetrahydrofuran, as mobile phases of HPLC. In many cases, applying these solvents increased the efficiency of separating enantiomers and allowed for obtaining a different selectivity profile for some racemic mixtures [ 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Liquid Crystalline Mixtures Enantioseparation on Polysaccharide-Based Chiral Stationary Phases by Reversed-Phase Chiral Liquid Chromatography

Urbańska

2024

IJMS

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Enantioseparation of nineteen liquid crystalline racemic mixtures obtained based on (R,S)-2-octanol was studied in reversed-phase mode on an amylose tris(3-chloro-5-methylphenylcarbamate) (ReproSil Chiral-MIG) and a cellulose tris(3,5-dichlorophenylcarbamate) (ReproSil Chiral-MIC). These polysaccharide-based chiral stationary phase (CSP) columns for High-Performance Liquid Chromatography (HPLC) were highly effective in recognizing isomers of minor structural differences. The mobile phase (MP), which consists of acetonitrile (ACN)/water (H2O) at different volume ratios, was used. The mobile phases were pumped at a flow rate of 0.3, 0.5, or 1 mL·min−1 with a column temperature of 25 °C, using a UV detector at 254 nm. The order of the elution was also determined. The chromatographic parameters, such as resolution (Rs), selectivity (α), and the number of theoretical plates, i.e., column efficiency (N), were determined. The polysaccharide-based CSP columns have unique advantages in separation technology, and this study has shown the potential usefulness of the CSP columns in separating liquid crystalline racemic mixtures belonging to the same homologous series.

show abstract

High-performance liquid chromatographic separation of the enantiomers of organophosphorus pesticides on polysaccharide chiral stationary phases

Cited by 88 publications

References 15 publications

Hydrolysis of chiral organophosphorus compounds by phosphotriesterases and mammalian paraoxonase-1

Hydrolysis of chiral organophosphorus compounds by phosphotriesterases and mammalian paraoxonase-1

Application of Polysaccharide‐Based Columns for the Separation of Racemic Mixtures Obtained Based on (R,S)‐2‐Octanol by Reversed‐Phase Chiral Liquid Chromatography

Optimization of Liquid Crystalline Mixtures Enantioseparation on Polysaccharide-Based Chiral Stationary Phases by Reversed-Phase Chiral Liquid Chromatography

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