High-performance liquid chromatographic separation of citraconylinsulins and preparation of GlyA1PheB1-dicitraconylinsulin.

Heme-Undecapeptide Labeling on Insulin for the Immunoassay of Insulin with Chemiluminescence Detection

“…(Osaka, Japan); Lyophilized porcine insulin was obtained as described previously. 9 A mouse anti-porcine insulin monoclonal antibody IgG solution in mouse ascites (IgG, 5.6 mg/ml) was obtained from Sekisui Chemical Co., Ltd (Osaka, Japan). The antibody has cross-reactivities with human and whale insulin, but not with rat insulin; Bio gel P-4 and polyacrylamide beads bearing carboxyl groups (diameter, 5 -10 µm) were from Bio-Rad Laboratories (Richmond, CA); aqueous urea solution for HPLC was deionized by mixing it with a mixed ionexchange resin, Serdolit MB (Serva Feinbiochemica, Heiderberg, Germany) before use.…”