High-performance liquid chromatographic separation of potato glycoalkaloids

Modification of the rapid high-performance liquid chromatographic method for the determination of potato glycoalkaloids

“…Past HPLC methods for glycoalkaloid analyses (Bushway et al, 1979;Morris and Lee, 1981) contained an ammonium precepitation clean-up step that is very time consuming. Recently Carman et al (1984) have developed a rapid procedure whereby extracted glycoalkaloids were cleaned up with C18 Sep-Paks.…”

.alpha.-Chaconine and .alpha.-solanine content of potato products and their stability during several modes of cooking

“…Glycoalkaloids were obtained from potato blossoms by using the method of Bushway et al (1980). These glycoalkaloids were separated into individual components for standards by HPLC (Bushway et al, 1979). Solvents used in extracting and partitioning were A.C.S.…”

“…This mixture was centrifuged at 12000# for 5 min. A 1-mL aliquot was removed for analysis of -chaconine and asolanine by HPLC (Bushway et al, 1979).…”

Liquid chromatography–tandem mass spectrometry method for the quantification of a potent H3 receptor antagonist conessine in serum and its application to pharmacokinetic studies

“…12 Initially, high performance liquid chromatography (HPLC) has very limited application in the field of steroid alkaloid analysis because of the lack of strong chromophore leading to the compulsion of refractive index based or UV detection at 200–210 nm or derivatization techniques. 13,14 The short detection limit also restricts the availability of elution solvents. In the recent years, liquid chromatography–tandem mass spectrometry (LC–MS/MS) has revolutionized measurement of steroidal alkaloids.…”