High‐Performance Liquid Chromatography Assay for Moxifloxacin in Bulk, Pharmaceutical Formulations and Serum: Application to In‐Vitro Metal Interactions

Abstract

A simple reversed phase HPLC method have been successfully developed and validated for the quantitative determination of moxifloxacin (MOX) in bulk material, pharmaceutical formulation and serum. Purospher STAR C18 (25 cm × 4.6 mm, 5 μm) and Discovery C18 (25 cm × 4.6 mm, 5 μm) columns were used. The mobile phase, methanol: acetonitrile: water (85:5:10, v/v/v), pH 2.75 adjusted by phosphoric acid was delivered at a flow rate of 1 mL min−1. The eluents was monitored using UV detector at 290 nm. The proposed method is specific, accurate (99.39‐102.67%), precise (intra‐day variation 0.04‐0.86% and inter‐day variation 0.12‐0.94%) and linearity (R2 > 0.999) within the desired range 0.39‐25 μg mL−1 concentration. The detection and quantification limit was 0.002‐0.51 μg mL−1 and 0.01‐0.555 μg mL−1, respectively. The analysis of variance (ANOVA) and student's t‐test were applied to verify the results. The anticipated method is applicable to routine analysis of MOX in pharmaceutical formulations and human serum samples. It is also applied on interaction of MOX with several essential and trace metals as well as interaction with antacids.

Monitoring of Pregabalin in Pharmaceutical Formulations and Human Serum Using UV and RP-HPLC Techniques: Application to Dissolution Test Method

“…The results show that buffer of 0.06 N HCl was the best dissolution medium, the immediate release of hard gelatin capsule was 100% highest drug release and the disintegration time for the capsule was 15 minute. The rotation speed 75 or 100 rpm was adjusted for capsules (Table 1) [14], and the percentage of drug discharge was calculated with the help of statistical parameter Student's-t distribution test at 0.05 significant levels and the most interesting thing is that the both stirring speeds are practicable and functional for both products for 75 mg and for 150 mg capsules".…”

“…These all were quite exclusive methods because of their extended and monotonous pretreatment of the samples, extraction with solvent and derivatization for the analysis of pregabalin. Our research group has reported a number of LC methods for the determination of various drugs as quinolones [13][14][15], cephalosporines [16], Ca channel blockers [17], ACE inhibitors [18][19][20][21] and antidiabetic drugs [22][23][24][25][26][27]. In continuation with this work we report are rapid, sensitive, selective, precise, short time analysis and cost effective RP-HPLC method for the quantitation of pragabalin in API, dosage formulations and human serum, the method is being validated according to ICH guidelines [28,29].…”

Synthesis, Characterization, Spectrofluorometric and Antibacterial Activity Studies of Moxifloxacin- Zirconium Complex

“…The methods employed in the literature include high-Performance Liquid Chromatography (20,21) ; square-wave voltammetry by interaction of MOX with Cu(II) (6) ; potentiometric and UV spectrophotometric measurements through complex formation with gadolinium(III) ion (22) . Recently, MOX-copper complexes were synthesized, characterized and screened for anti-proliferative and apoptosis-inducing activity against multiple human breast cancer cell lines (23) .…”

Fragmentation of Moxifloxacin and Its Analogs by Electrospray Ionization Time-of-Flight Mass Spectrometry

“…Moxifloxacin (1-cyclopropyl-7-[(1S, 6S)-2, 8-diazabicyclo[4.3.0]non-8-yl]-6-fluoro-8-methoxy-4-oxo-quinoline-3-carboxylic acid) is a fourth-generation quinolone that has attracted the attention of chemists, as its pharmacokinetics and in vivo metabolism have been been thoroughly investigated (Vishwanathan, Bartlett, and Stewart 2002;Vu et al 2011;Sultana et al 2010;Xu et al 2010;Hemanth et al 2011). Raju et al (2012) recently determined the fragmentation pathways of moxifloxacin by electrospray ionization (ESI)-quadrupole ion trap mass spectrometry and ESI-quadrupole-time-of-flight (QTOF).…”