High-performance liquid chromatography–atmospheric-pressure chemical ionization mass spectrometry as a new tool for the determination of the mycotoxin zearalenone in food and feed

Rosenberg, Erwin; Krska, Rudolf; Wissiack, René; Kmetov, Veselin; Josephs, R; Razzazi, E; Grasserbauer, M.

doi:10.1016/s0021-9673(98)00462-2

Cited by 65 publications

(31 citation statements)

References 20 publications

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“…The column thermostat was set to 26°C and the parameters for the APCI interface were optimized as described elsewhere. 20 The measurements were performed in positive and negative ion mode at 450°C vaporizer temperature, 350°C drying gas temperature, 5 L/min drying gas flow, 50 psig drying gas pressure, and 4000 V capillary voltage. The corona current was set to 6 mA in the positive ion mode, and to 10 mA in the negative ion mode, respectively.…”

Section: Methodsmentioning

confidence: 99%

In-source fragmentation of partially oxidized mono- and polycyclic aromatic hydrocarbons in atmospheric pressure chemical ionization mass spectrometry coupled to liquid chromatography

Letzel

Pöschl

Rosenberg

et al. 1999

Rapid Commun. Mass Spectrom.

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Partially oxidized derivatives of polycyclic aromatic hydrocarbons (PAHs) are known to be important environmental pollutants. For the identification of these substances in complex mixtures, e.g. atmospheric aerosol samples, liquid chromatography/mass spectrometry with atmospheric pressure chemical ionization (LC/APCI-MS) has been found to be a suitable analytical technique. In this study 31 derivatives of mono- and polycyclic aromatic hydrocarbons with up to five condensed aromatic rings carrying different functional groups (carboxyl, dicarboxylic anhydride, lactone, hydroxyl, and carbonyl) were characterized by LC/APCI-MS. Each substance was measured in positive and negative ion detection mode at four different fragmentor voltages (90 to 190 V). For the first time, the results show that characteristic and well-interpretable fragmentation patterns can be obtained for these classes of compounds by in-source collision-induced dissociation in a single quadrupole LC/APCI-MS system. For each class of compounds typical spectral features and optimum measurement conditions are reported, and fragmentation pathways are proposed. The study demonstrates the applicability of LC/APCI-MS for the determination of most of the investigated compounds at trace levels, and it provides a database for the identification of unknown partially oxidized aromatic hydrocarbons. Copyright 1999 John Wiley & Sons, Ltd.

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Section: Methodsmentioning

confidence: 99%

In-source fragmentation of partially oxidized mono- and polycyclic aromatic hydrocarbons in atmospheric pressure chemical ionization mass spectrometry coupled to liquid chromatography

Letzel

Pöschl

Rosenberg

et al. 1999

Rapid Commun. Mass Spectrom.

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“…In some cases farmers have exploited the potential of mycotoxins and used it to promote fattening of sheep and cattle (Yang and Bittner, 2002). Methods are being developed to detect mycotoxins in foodstuffs and feedstuffs to safeguard both animal and human health (Rosenberg et al, 1998). The United States Food and Drug Administration (FDA) has not established advisory limits nor action levels for zearalenone, although if this mycotoxin is present usually other mycotoxins for which the FDA has set advisory levels are also present, such as deoxynivalenol produced by Fusarium spp.…”

Section: Other Estrogenic Compoundsmentioning

confidence: 99%

Phytoestrogens: a review of the present state of research

Ososki

Kennelly

2003

Phytotherapy Research

407

278

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Phytoestrogens are a diverse group of plant-derived compounds that structurally or functionally mimic mammalian estrogens and show potential benefits for human health. The number of articles published on phytoestrogens has risen dramatically in the past couple decades. Further research continues to demonstrate the biological complexity of phytoestrogens, which belong to several different chemical classes and act through diverse mechanisms. This paper discusses the classification of phytoestrogens, methods of identification, their proposed mechanisms of action and botanical sources for phytoestrogens. The effects of phytoestrogens on breast and prostate cancers, cardiovascular disease, menopausal symptoms and osteoporosis will also be examined including research on benefits and risks.

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“…Electrospray (ESI) and atmospheric pressure chemical ionization (APCI) interfaces offer the advantages of robustness and easy handling, important requirements for routine LC-MS analysis with high sample throughput. In this context, with the use of an APCI interface, limits of detection in ppt range could be achieved for the determination of ZON in maize [ 19,20] and ZON and ZOLs in beer [21]. For sample clean-up a solid-phase extraction (SPE) step was performed prior to the LC-MS analysis [20,21 ].…”

Section: Introductionmentioning

confidence: 99%

Determination of zeranol, taleranol, zearalenone, α- and β-zearalenol in urine and tissue by high-performance liquid chromatography-tandem mass spectrometry

2000

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Key WordsColumn liquid chromatography Tandem mass spectrometry Zeranol, zearalenone and zearalenol SummaryAn LC-MS/MS method has been developed for the sensitive determination of the anabolic compound zeranol, its epimer taleranol and the mycotoxins zearalenone, azearalenol and fl-zearalenol in animal urine and meat samples. Sample preparation included extraction of meat samples and enzymatic digest of urine samples followed by solid phase extraction with RP-18 columns for sample clean-up. Mass spectrometric determination was carried out with an atmospheric pressure chemical ionisation interface (APCI) in the multi-reaction monitoring mode (MRM). Using the negative ion mode, a limit of detection between 0.1 and 0.5 ppb and a limit of determination between 0.5 and 1 ppb could be achieved. With zearalanone (ZAN) as internal standard, a linear range between 0.5 (1.0) and 100ppb in urine samples (cow, pig) and between 1 and 100 ppb in meat samples (cow, calf, pig) could be established. Depending on the biological matrix and analyte, standard deviations were below 8.5 %, with average recovery rates around 86 % to 102 % in spiked samples. The usefulness of the method developed was demonstrated via several contaminated cow and pig urine samples. the mycotoxins zearalenone (ZON) and e-zearalenol (7-ZOL) that can be carried over from contaminated feedstuffto animals [2][3][4]. ZON, e-ZOL and its epimer/%zearalenol (fl-ZOL) are produced by several Fusarium species that colonize grains [5]. High amounts can be found on maize, wheat, barley and oats. All three compounds exhibit distinct HC

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High-performance liquid chromatography–atmospheric-pressure chemical ionization mass spectrometry as a new tool for the determination of the mycotoxin zearalenone in food and feed

Cited by 65 publications

References 20 publications

In-source fragmentation of partially oxidized mono- and polycyclic aromatic hydrocarbons in atmospheric pressure chemical ionization mass spectrometry coupled to liquid chromatography

In-source fragmentation of partially oxidized mono- and polycyclic aromatic hydrocarbons in atmospheric pressure chemical ionization mass spectrometry coupled to liquid chromatography

Phytoestrogens: a review of the present state of research

Determination of zeranol, taleranol, zearalenone, α- and β-zearalenol in urine and tissue by high-performance liquid chromatography-tandem mass spectrometry

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