High-performance liquid chromatography—chemiluminescence determination of methamphetamine in human serum using N-(4-aminobutyl)-N-ethylisoluminol as a chemiluminogen

Nakashima, Kenichiro; Suetsugu, Keiko; Akiyama, Shuzo; Yoshida, Masanori

doi:10.1016/s0378-4347(00)82315-4

Cited by 37 publications

(8 citation statements)

References 13 publications

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“…Recently, we have been aiming at developing selective and sensitive analytical procedures for the determination of these compounds in biological samples (Nakashima et al, 1990(Nakashima et al, , 1991(Nakashima et al, , 1992Al-Dirbashi et al, 1997;Kuroda et al, 1998). Higher sensitivities with simple sample pretreatment and derivatization procedures could be attained when MP and its related compounds were analyzed in biological samples either on a reversed-phase or a chiral stationary phase packed column (Al-Dirbashi et al, 1998a, b, 1999a as derivatives of our previously developed fluorescence labeling reagent, namely, 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride (DIB-Cl) (Nakashima et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorescence detection; applications on abusers' single hair analyses

Al-Dirbashi

Kuroda

Wada

et al. 2000

Biomed. Chromatogr.

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Achiral and chiral semi-micro column high-performance liquid chromatographic methods with fluorescence detection to determine methamphetamine and amphetamine in human hair are described. These compounds were extracted into 5% trifluoroacetic acid (TFA) in methanol, derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride and separated either on a 250 x 1.5 mm i.d. octadecyl-silane (ODS) or a 150 x 2 mm i.d. OD-RH column. Linear calibration curves extending over a wide range of concentration that covers the practical samples were obtained for amphetamine, methamphetamine and their enantiomers (r = 0.999). Resolution values for amphetamine and methamphetamine enantiomers were 3.4 and 1.1, respectively. Intra- and inter-day variations of both the methods were not larger than 8.9% expressed as relative standard deviations (n >/= 5). The limits of detection at a signal-to-noise ratio of 3 obtained by both the methods were in the range of 1.0-4.7 fmol/5 microL injection with the achiral method being more sensitive. Abusers' hair samples were analyzed by the two methods and only the S(+)-enantiomers were found in eight Japanese abusers' hair samples. The achiral method was used to study the concentrations of these compounds in single black and white hair strands of abusers.

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Section: Introductionmentioning

confidence: 99%

Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorescence detection; applications on abusers' single hair analyses

Al-Dirbashi

Kuroda

Wada

et al. 2000

Biomed. Chromatogr.

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“…In hair samples, AP and MP could also be detected in very low concentrations. al., , Jonsson et al, 1996Herraez-Hernandez et al, 1998), in addition to plasma (Cheung et al, 1997;Nakashima et al, 1990;Nagasawa et al, 1996;Gjerde et al, 1993;Hara et al, 1997;Bogusz et al, 1997) and hair (Nakahara et al, 1993;Slawson et al, 1996;Takayama et al, 1997), have been detailed.Recently, we reported highly sensitive HPLC methods with fluorescence (FL) detection for the determination of AP and MP in urine (Al-Dirbashi et al, 1998a,b, 2000a and hair samples (Al-Dirbashi et al, 1999, 2000b obtained from MP abusers. In these methods, AP and MP were determined after their derivatization with the fluorescent reagent, 4-(4,5-diphenyl-1H-imidazole-2-yl)benzoyl chloride (DIB-Cl) (Nakashima et al, 1995).…”

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Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC‐FL

Nakashima

Kaddoumi

Ishida

et al. 2003

Biomedical Chromatography

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This paper describes highly sensitive HPLC methods for the determination of amphetamine (AP) and methamphetamine (MP) in abusers' plasma and hair samples. AP and MP were derivatized with the fluorescent reagent, DIB-Cl, to yield a highly fluorescent DIB-derivatives of AP and MP, which were then analyzed by HPLC with fluorescence detection at excitation and emission wavelengths of 325 and 430 nm, respectively. The separation was achieved on an ODS column with isocratic mobile phases composed of acetoniltrile and citrate buffer (55:45, v/v) for plasma samples and of acetonitrile-methanol-citrate buffer (45:20:37.5, v/v/v) for hair samples. The limits of detection were less than 0.87 ng/mL and 0.12 ng/mg in plasma and hair samples, respectively, for both AP and MP. The methods were then applied to the determination of MP and its metabolite AP in plasma obtained from two cases of illegally ingested MP and in one of the cases' hair received later. Case I was treated with dialysis; samples before and after dialysis were analyzed by the described method. After dialysis for 5 h, the total plasma levels of AP and MP decreased from 720 to 190 ng/mL. For case II, MP and AP levels were monitored for 3 days after digestion. Total plasma levels decreased from 57 ng/mL in the day of digestion to 11 ng/mL after 3 days. In hair samples, AP and MP could also be detected in very low concentrations.

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“…It's known that there are dansyl chloride (Dns-Cl), 4-fluoro-7-nitro-2,1,3-benzontinezole (NBD-F), 4-chloro-7-nitro-2,1,3-benzontinezole (NBD-Cl), naphthalene-2,3-dialdehyde (NDA) and o-phthaldialdehyde as the derivatizing reagents. Meanwhile, N-(4-aminobutyl)-N-ethylisoluminol (ABEI) [129] and luminarins 1 and 2 [130] (see Fig. 13) are used as chemiluminogenic labeling reagents for the sensitive determination of amines.…”

Section: Chemiluminescence Probesmentioning

confidence: 99%

Advances in the study of luminescence probes for proteins

Sun

Yang

et al. 2004

Journal of Chromatography B

103

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High-performance liquid chromatography—chemiluminescence determination of methamphetamine in human serum using N-(4-aminobutyl)-N-ethylisoluminol as a chemiluminogen

Cited by 37 publications

References 13 publications

Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorescence detection; applications on abusers' single hair analyses

Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorescence detection; applications on abusers' single hair analyses

Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC‐FL

Advances in the study of luminescence probes for proteins

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