2009
DOI: 10.1590/s0102-695x2009000500011
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High performance liquid chromatography determination of cucurbitacins in the roots of Wilbrandia ebracteata Cogn.

Abstract: RESUMO: "Determinação de cucurbitacinas em raízes de Wilbrandia ebracteata Cogn. por cromatografia líquida de alta eficiência". Raízes de Wilbrandia ebracteata Cogn. (Cucurbitaceae), tradicionalmente empregada no tratamento de doenças reumáticas, contém cucurbitacinas, sendo di-hidrocucurbitacina B a mais abundante, enquanto cucurbitacina B está presente em menor quantidade. Foi desenvolvido um método para determinação quantitativa destas cucurbitacinas. Os parâmetros selecionados foram: eluente isocrático ace… Show more

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Cited by 10 publications
(6 citation statements)
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“…The flow rate of the mobile phase was 1 ml/min, and the detection wavelength was 200 nm. Similarly, pure cucurbitacin B and bottle gourd ultrafiltered homogenate were subjected to HPLC following the method by Krepsky et al with slight modification [44] . Acetonitrile/water at a ratio of 40 : 60 was used as the mobile phase at a flow rate of 1.2 ml/min.…”
Section: Methodsmentioning
confidence: 99%
“…The flow rate of the mobile phase was 1 ml/min, and the detection wavelength was 200 nm. Similarly, pure cucurbitacin B and bottle gourd ultrafiltered homogenate were subjected to HPLC following the method by Krepsky et al with slight modification [44] . Acetonitrile/water at a ratio of 40 : 60 was used as the mobile phase at a flow rate of 1.2 ml/min.…”
Section: Methodsmentioning
confidence: 99%
“…The chromatographic method was developed based on the previous method with some modification [25] . The RP-HPLC method was refined by changing the mobile phase composition in a gradient manner and finally, isocratic method was optimized with the mobile phase of acetonitrile (solvent A) and water (solvent B) in the ratio of 70: 30 (v/v).…”
Section: Methodsmentioning
confidence: 99%
“…The chromatographic conditions were, C18 column (Phenomenex Kinetix ® ; 5 m, 150 mm × 4.6 mm), mobile phase water:acetonitrile (60:40%), isocratic mode, at a flow rate of 1.2 mL/min. Temperature of 40 • C and reading at 230 nm (Krepsky et al, 2009). …”
Section: Dihydrocucurbitacin B Assaymentioning
confidence: 99%