High-performance liquid chromatography determination of phenyllactic acid in MRS broth

Armaforte, Emanuele; Carri, Simone; Ferri, Gabriele; Caboni, Maria Fiorenza

doi:10.1016/j.chroma.2006.07.095

Cited by 29 publications

(27 citation statements)

References 19 publications

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“…It is of concern that most published methods report only analysis of the SPE eluent, which considering the vast differences in the polarity of the compounds will almost certainly have led to substantial losses during the load and wash steps. This observation was previously described by Armaforte [15] who reported 10% recovery of phenyllactic acid in the elute step and clear preference for the compound in the wash step. Aramforte compared direct injection with SPE and showed that reproducibility and recovery of phenyllactic acid were much better after direct injection.…”

Section: Matrix Effectssupporting

confidence: 71%

“…Minimal matrix effects were observed. Previous methods for extraction of LABs used either protracted sample-preparation techniques, for example SPE or LLE, or direct sample injection [3,4,[11][12][13][14][15][16]. In this work, LLE and direct injection with standards prepared in the mobile phase were compared.…”

Section: Matrix Effectsmentioning

confidence: 99%

“…Other metabolites from these bacteria have been identified as the source of the antifungal activity. These include cyclic dipeptides, proteinaceous compounds, fatty acids and a variety of other low-molecular-weight compounds [3,4,[11][12][13][14][15][16]. The individual MIC of these metabolites are much higher than the amounts detected in culture broths.…”

Section: Introductionmentioning

confidence: 97%

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Rapid identification, by use of the LTQ Orbitrap hybrid FT mass spectrometer, of antifungal compounds produced by lactic acid bacteria

et al. 2012

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Fungal contamination of food causes health and economic concerns. Several species of lactic acid bacteria (LAB) have antifungal activity which may inhibit food spoilage fungi. LAB have GRAS (generally recognised as safe) status, allowing them to be safely integrated into food systems as natural food preservatives. A method is described herein that enables rapid screening of LAB cultures for 25 known antifungal compounds associated with LAB. This is the first chromatographic method developed which enables the rapid identification of a wide range of antifungal compounds by a single method with a short analysis time (23 min). Chromatographic separation was achieved on a Phenomenex Gemini C18 100A column (150 mm × 2.0 mm; 5 μm) by use of a mobile-phase gradient prepared from (A) water containing acetic acid (0.1%) and (B) acetonitrile containing acetic acid (0.1%), at a flow rate of 0.3 µL min(-1). The gradient involved a progressive ramp from 10-95% acetonitrile over 13 min. The LC was coupled to a hybrid LTQ Orbitrap XL fourier-transform mass spectrometer (FTMS) operated in negative ionisation mode. High mass accuracy data (<3 ppm) obtained by use of high resolution (30,000 K) enabled unequivocal identification of the target compounds. This method allows comprehensive profiling and comparison of different LAB strains and is also capable of the identification of additional compounds produced by these bacteria.

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Section: Matrix Effectssupporting

confidence: 71%

Section: Matrix Effectsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Rapid identification, by use of the LTQ Orbitrap hybrid FT mass spectrometer, of antifungal compounds produced by lactic acid bacteria

et al. 2012

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“…It was found that LAB produced PhLA as a reaction product of amino acid metabolism [22,23]. Its detection by reverse-phase HPLC is simple and widely adopted method for quantitative measurement of PhLA present in fermentation broth [24], honey [19] and rumen fluid [25]. GC/MS and capillary electrophoresis were also used for quantitative measurement of PLA.…”

Section: Phla Occurrence and Its Detectionmentioning

confidence: 99%

Phenyllactic Acid: A Potential Antimicrobial Compound in Lactic acid Bacteria

Ss¹

2016

JBMOA

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Abstract3-Phenyllactic acid (PhLA) is a broad spectrum antimicrobial compound active against bacteria and fungi. PhLA exists in two chiral isomers, L-PhLA and D-PhLA which show antimicrobial activity. D-PhLA shows more antimicrobial activity than L-PhLA and hence it is receiving great attention as food and feed additive in place antibiotics which control the microbial contamination and thereby increase the shelf life of food and food ingredients. This review summarizes the recent developments on the resources, detection and analysis and antimicrobial activity of PhLA. In addition, the article presents the recent studies of the key enzymes such as lactate dehydrogenases and pyruvate reductases involved in PhLA synthesis. The metabolic pathway and regulation of PLA synthesis in lactic acid bacteria (LAB) along with its high level production are also discussed.

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“…A rapid method of HPLC (Armaforte et al 2006) was used to separate the antiyeast compounds in the cell free culture medium. The R-HPLC, was equipped with a quanternary pump, diode array detector set at 210 nm, a Hypersil ODS column C18 RP (250 x 4.0 mm, HP, particle size 5 µm) and run at room temperature.…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages

Kantachote¹,

Prachyakij²,

Charernjiratrakul³

et al. 2010

Electron. J. Biotechnol.

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Lactobacillus plantarum DW3 produced antifungal compounds that inhibited the growth of Rhodotorula mucilaginosa DKA, contaminating yeast in fermented plant beverages (FPBs) and various potential human pathogens. Phenyllactic acid (PLA) identified by gas chromatography-mass spectrometry (GC-MS) was produced at 31 mg/L PLA in MRS medium and 5 mg/ml inhibited growth of the target yeast in vitro by 90%. Other inhibitors were also present but not specifically identified. Results of in vitro tests showed that DW3 also had probiotic properties as it survived various human biological barriers resistance to pH 3, bile salts, growth without vitamin B12 and the presence and absence of oxygen. Its inhibitory effect against foodborne pathogenic bacteria and spoilage organisms was higher than that found for a commercial strain Lactobacillus casei R. An acute oral toxicity test on ICR mice at a high single dose of either 10 9 and 10 12 cells per mouse for 14 days showed that DW3 had no adverse effect on the general health status and there was no evidence of bacteremia. Mice fed DW3 had a reduced weight gain compared to the control. No significant difference (p > 0.05) was found for the spleen weight index (SWI) among the treatment and control groups whereas there was a significant difference (p < 0.05) for the liver weight ratio (LWR) in a group fed with 10 12 cells per mouse when compared with the control group.

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High-performance liquid chromatography determination of phenyllactic acid in MRS broth

Cited by 29 publications

References 19 publications

Rapid identification, by use of the LTQ Orbitrap hybrid FT mass spectrometer, of antifungal compounds produced by lactic acid bacteria

Rapid identification, by use of the LTQ Orbitrap hybrid FT mass spectrometer, of antifungal compounds produced by lactic acid bacteria

Phenyllactic Acid: A Potential Antimicrobial Compound in Lactic acid Bacteria

Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages

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