High performance liquid chromatography evaluation of serum carbohydrate-deficient transferrin and more sialylated transferrin glycoforms in children

Bianchi, Vincenza; Raspagni, Alessia; Arfini, Carlo; Vidali, Matteo

doi:10.3109/00365513.2012.660537

Cited by 5 publications

(3 citation statements)

References 45 publications

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“…This is due to the fact that probably the glycoform with a lower content of sialic acid are more saturated with iron, useful for the synthesis of hemoglobin which carries oxygen to the foetus. The distribution of disialotransferrin and other glycoforms with the highest content of sialic acid has been also studied in children and adolescents [13].…”

Section: Carbohydrate Deficient Transferrin (Cdt)mentioning

confidence: 99%

Emerging Biomarkers of Alcohol Consumption: Clinical and Forensic Applications

Bianchi¹,

Raspagni²,

Arfini³

2013

TOTOXIJ

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Alcohol-related problems are typically associated with medical, economic and social issues. The reduction of the problem can only occur if effective strategies to prevent, diagnose and treat alcohol abuse are developed. Real help could come from laboratory tests that represent objective evidence of alcohol problems. Authors present new biomarkers as Carbohydrate-deficient transferrin (CDT), Ethyl Glucuronide and Ethyl Sulfate (EtG and EtS)), Fatty Acids Ethyl Esters (FAEE) anf Phosphatidy ethanol (PEth) in differents biological samples.

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Section: Carbohydrate Deficient Transferrin (Cdt)mentioning

confidence: 99%

Emerging Biomarkers of Alcohol Consumption: Clinical and Forensic Applications

Bianchi¹,

Raspagni²,

Arfini³

2013

TOTOXIJ

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“…Ciò è dovuto al fatto che probabilmente le forme a più basso contenuto di acido sialico sono quelle più sature di ferro utile alla sintesi dell'emoglobina che porta ossigeno al feto. E' stata studiata anche la distribuzione della disialotransferrina e delle altre glicoforme a più alto contenuto di acido sialico nei bambini e adolescenti (Bianchi et al 2012).…”

Section: Transferrina Carboidrato Carente (Cdt) O Transferrina Desialataunclassified

Identificazione precoce dell’abuso di alcol: utilizzo dei biomarcatori emergenti in differenti matrici biologiche

Bianchi¹,

Arfini²

2012

Work Paper Public Hlth

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Scopo: Questa rassegna illustra alcuni biomarcatori emergenti misurabili in diverse matrici biologiche che possono permettere l’individuazione precoce dell’abuso alcolico; Metodologia: vengono considerati i biomarcatori più attuali transferrina carboidrato carente (CDT), etilglucuronide (EtG), fosfatidiletanolo (PEth) e gli esteri etilici degli acidi grassi (FAEE) e la loro determinazione nelle diverse matrici biologiche così come è riportato nella più recente letteratura; Risultati: sono descritte alcune situazioni reali ed è suggerita l’interpretazione dei risultati. Conclusioni: la rassegna fornisce suggerimenti e indicazioni per il corretto uso dei biomarcatori descritti alla luce delle conoscenze e delle applicazioni cliniche e forensi.

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“…Variations in the number of terminal sialic acid residues influence the electrophoretic mobility of the Tf molecule and thus permit analytic separation of the different isoforms . Among the human Tf isoforms, which vary from asialo‐Tf and octasialo‐Tf, the most common distribution observed in nonalcohol drinkers is tetra‐Tf (79.9%), followed by pentasialo‐Tf (14.0%), trisialo‐Tf (4.02%), disialo‐Tf (1.14%), and hexasialo‐Tf (0.73%) in order . In contrast, chronic alcohol abusers show an increase in the disialo‐Tf and asialo‐Tf fractions, which constitute the so‐called carbohydrate‐deficient transferrin (CDT).…”

Section: Introductionmentioning

confidence: 99%

The characteristics of transferrin variants by carbohydrate‐deficient transferrin tests using capillary zone electrophoresis

Yoo

Kim

Yoon

2018

Clinical Laboratory Analysis

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Background Transferrin is the major plasma transport protein for iron. We aimed to investigate the characteristics of transferrin variant by carbohydrate‐deficient transferrin (CDT) test using capillary zone electrophoresis. Methods We retrospectively analyzed the CDT tests of 2449 patients from March 2009 to May 2017 at a tertiary hospital in Korea. CDT was quantified using a Capillarys 2 system (Sebia, Lisses, France) by capillary zone electrophoresis. The characteristics of variant transferrin patterns using electropherogram of CDT tests were analyzed. Results Seventy‐seven (3.1%) patients were classified as variant transferrin. Mean age of these patients was 51.8 years, and the male‐to‐female ratio was 3.5:1. The most common variants were the BC variants (n = 37), followed by the CD variants (n = 27), unclear patterns (n = 7), BD variants (n = 3), CC variants (n = 2), misclassification (n = 1). In the variant Tf group, the most common disease was alcoholic liver cirrhosis (n = 22, 28.6%), followed by the toxic effects of substances (n = 17, 22.1%), and mental and behavioral disorders attributable to alcohol (n = 11, 14.3%). Nonvariant group showed a predominance of the toxic substance effects (n = 880, 37.1%), a personal history of suicide attempts (n = 634, 26.7%), and mental and behavioral disorders due to alcohol (n = 336, 14.2%). Conclusion We analyzed the basic characteristics of variant transferrin by CDT tests using capillary zone electrophoresis. The prevalence of variant transferrin was 3.1% of the study subjects. Male patients, alcohol abusers, and liver cirrhosis patients predominated in the variant transferrin population. Further prospective studies are warranted to elucidate variant transferrin in clinical practice.

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High performance liquid chromatography evaluation of serum carbohydrate-deficient transferrin and more sialylated transferrin glycoforms in children

Cited by 5 publications

References 45 publications

Emerging Biomarkers of Alcohol Consumption: Clinical and Forensic Applications

Emerging Biomarkers of Alcohol Consumption: Clinical and Forensic Applications

Identificazione precoce dell’abuso di alcol: utilizzo dei biomarcatori emergenti in differenti matrici biologiche

The characteristics of transferrin variants by carbohydrate‐deficient transferrin tests using capillary zone electrophoresis

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