High-performance liquid chromatography in the radioimmunoassay of urinary tetrahydroaldosterone

Holland, O. B.; Risk, Martin; Brown, H; Komes, Kevin; Dube, Paula; Swann, Cheryl

doi:10.1016/s0021-9673(01)94655-2

Cited by 5 publications

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“…It is primarily a liver metabolite, corresponding to around half of the aldosterone metabolites [67,87]. Measuring tetrahydroaldosterone in the urine involves hydrolysis of the glucuronide conjugate with a beta-glucuronidase and measurement by several methods, including radioimmunoassay, highperformance liquid chromatography (HPLC)-MS-MS [88][89][90][91][92][93]. Since most aldosterone is metabolized to 3α,5β-tetrahydroaldosterone, it has been proposed that it is a better indicator of aldosterone secretion; however, the metabolism of aldosterone to the 3α,5βtetrahydroaldosterone is affected by different disease states; in particular, it is decreased in congestive heart failure and cirrhosis of the liver [94,95].…”

Section: Tetrahydroaldosteronementioning

confidence: 99%

An Abbreviated History of Aldosterone Metabolism, Current and Future Challenges

Gómez-Sánchez

2023

Exp Clin Endocrinol Diabetes

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The initial isolation of adrenal steroids from large quantities of animal adrenals resulted in an amorphous fraction resistant to crystallization and identification that had potent effects on electrolyte transport. Aldosterone was eventually isolated and identified in the fraction and was soon shown to cause hypertension when in excess. The autonomous and excessive production of aldosterone, primary aldosteronism, is the most common cause of secondary hypertension. Aldosterone is metabolized in the liver and kidney and its metabolites conjugated with glucuronic acid for excretion. The most common liver metabolite is 3,5-tetrahydroaldosterone-3-glucuronide; that of the kidney is aldosterone-18-oxo-glucuronide. Their values, especially the aldosterone-18-oxo-glucuronide, are commonly used for the diagnosis of primary aldosteronism because they provide and integrated value of the total daily production of aldosterone. Conversion of aldosterone to 18-oxo-glucuronide is impeded by drugs, like some common NSAIDS that compete for UGT2B7, the most important glucuronosyltransferase for aldosterone metabolism. Tetrahydroaldosterone is the most abundant metabolite and most reliable for the diagnosis of primary aldosteronism, but is not commonly measured.

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Section: Tetrahydroaldosteronementioning

confidence: 99%

An Abbreviated History of Aldosterone Metabolism, Current and Future Challenges

Gómez-Sánchez

2023

Exp Clin Endocrinol Diabetes

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“…RIA is not very selective for THALD, owing to the cross-reactivity of the THALD antibody with tetrahydrocortisol and tetrahydrocortisone. Recently, a RIA method has been coupled with high performance liquid chromatography (HPLC) to separate THALD from the cross-reacting steroids before RIA (Holland et al, 1987). The method, however, is time consuming and requires a radioactive compound.…”

Section: Introductionmentioning

confidence: 99%

High performance liquid chromatographic determination of 3α, 5β‐tetrahydroaldosterone and cortisol in human urine with fluorescence detection

Yoshitake

Ishida

Sonezaki

et al. 1992

Biomedical Chromatography

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A simple and sensitive method for the simultaneous determination of 3 alpha, 5 beta-tetrahydroaldosterone (THALD) and cortisol in human urine is described. The method uses high performance liquid chromatography with fluorescence detection. THALD and cortisol, released by enzyme hydrolysis, and fludrocortisone (internal standard) are isolated by a Sephadex G-25M column and a Bond-Elut C18 cartridge, and then oxidized by cupric acetate to form the corresponding glyoxal derivatives. The glyoxal derivatives are converted into the fluorescent quinoxalines by reaction with 1,2-diamino-4,5-methylenedioxybenzene. The quinoxalines are successfully separated on a reversed phase column (L-column ODS) with isocratic elution and monitored fluorimetrically. The detection limits for THALD and cortisol are 0.45 and 1.18 ng/mL urine (0.65 and 2.65 pmol/100 microL injection volume), respectively, at a signal-to-noise ratio of 3 in a 100 microL injection volume. This method permits the precise and sensitive determination of THALD and cortisol in human urine (2 mL).

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Chapter IV Radio-Column Liquid Chromatography

Veltkamp¹

1989

Journal of Chromatography Library

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High-performance liquid chromatography in the radioimmunoassay of urinary tetrahydroaldosterone

Cited by 5 publications

References 0 publications

An Abbreviated History of Aldosterone Metabolism, Current and Future Challenges

An Abbreviated History of Aldosterone Metabolism, Current and Future Challenges

High performance liquid chromatographic determination of 3α, 5β‐tetrahydroaldosterone and cortisol in human urine with fluorescence detection

Chapter IV Radio-Column Liquid Chromatography

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