High-performance liquid chromatography–mass spectrometry and evaporative light-scattering detector to compare phenolic profiles of muscadine grapes

You, Qidong; Chen, Feng; Sharp, Julia L.; Xi, Wang; You, Yu; Zhang, Chuanji

doi:10.1016/j.chroma.2012.03.086

Cited by 27 publications

(24 citation statements)

References 32 publications

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“…A good correlation (linear fit R 2 > 0.99) was obtained for all classes at the concentration range evaluated in this work. Similar linear response has been observed by others for lipids and phenolic compounds [21, 25, 26]. Although ELSD is a universal detector, it is of note that the range of linearity depends on the lipid classes since the response of ELSD varies with the physical properties of lipids.…”

Section: Resultssupporting

confidence: 83%

Off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry to improve coverage in lipidomics analysis

Narváez-Rivas

Chen

et al. 2017

Analytica Chimica Acta

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The confident identification and in-depth profiling of molecular lipid species remain to be a challenge in lipidomics analysis. In this work, an off-line two-dimensional mixed-mode and reversed-phase liquid chromatography (RPLC) method combined with high-field quadrupole orbitrap mass spectrometer (Q Exactive HF) was developed to profile lipids from complex biological samples. In the first dimension, 22 different lipid classes were separated on a monolithic silica column with elution order from neutral to polar lipids. A total of 13 fractions were collected and run on a RPLC C30 column in the second dimension for further separation of the lipid molecular species based on their hydrophobicity, with the elution order being determined by both the length and degree of unsaturation in the fatty-acyl chain. The method was applied to analyze lipids extracted from rat plasma and rat liver. Fatty acid methyl ester analysis by gas chromatography-mass spectrometry was used to identify the fatty acyls from total lipid extracts, which provided a more confident identification of the lipid species present in these samples. More than 800 lipids were identified in each sample and their molecular structures were confidentially confirmed using tandem mass spectrometry (MS/MS). The number of lipid molecular species identified in both rat plasma and rat liver by this off-line two-dimensional method is approximately twice of that by one-dimensional RPLC-MS/MS employing a C30 column. This off-line two-dimensional mixed-mode LC-RPLC-MS/MS method is a promising technique for comprehensive lipid profiling in complex biological matrices.

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Section: Resultssupporting

confidence: 83%

Off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry to improve coverage in lipidomics analysis

Narváez-Rivas

Chen

et al. 2017

Analytica Chimica Acta

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“…This study reported that the majority of flavan-3-ols monomers was mainly found in skin and pulp of berries, while the majority of PAs was revealed in seeds. In addition, other studies, which used HPLC coupled with ultraviolet and a mass spectrometer (HPLC-UV-MS), HPLC coupled with an evaporative light scattering detection (HPLC-ELSD), and HPLC-UV-ESI-MS approaches to analyze flavan-3-ols and PAs in two cultivars, Noble and Carlos, supported this tissue-associated flavan-3-ols and PAs profile differentiation [18]. A recent comparative study revealed that field growth in China and USA differentially regulated flavan-3-ols and PAs profiles in muscadine grapes.…”

Section: Introductionmentioning

confidence: 94%

HPLC-qTOF-MS/MS-Based Profiling of Flavan-3-ols and Dimeric Proanthocyanidins in Berries of Two Muscadine Grape Hybrids FLH 13-11 and FLH 17-66

2018

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FLH 13-11 FL and FLH 17-66 FL are two interspecific hybrid varieties of muscadine grape resulting from the cross of Vitis munsoniana (Simpson) ex Munson and V. rotundifolia. However, profiles of flavan-3-ols and proanthocyanidins in these two hybrids have not been characterized. Herein, we report the use of high-performance liquid chromatography-quadrupole, time-of-flight, tandem mass spectrometry (HPLC-qTOF-MS/MS) to characterize these two groups of metabolites in berries. Ripe berries collected from two consecutive cropping years were used to extract metabolites. Metabolites were ionized using the negative mode. Collision-induced dissociation was performed to fragmentize ions to obtain feature fragment profiles. Based on standards, MS features, and fragments resulted from MS/MS, four flavan-3-ol aglycones, 18 gallated or glycosylated conjugates, and eight dimeric procyanidins, were annotated from berry extracts. Of these 30 metabolites, six are new methylated flavan-3-ol gallates. Furthermore, comparative profiling analysis showed obvious effects of each cultivar on the composition these 30 metabolites, indicating that genotypes control biosynthesis. In addition, cropping seasons altered profiles of these metabolites, showing effects of growing years on metabolic composition. These data are informative to enhance the application of the two cultivars in grape and wine industries in the future.

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“…Different results between UV, MS, and ELSD quantification have already been reported. 27) Among these detectors, ELSD is the most universal one used, because neither the optical properties of the compounds nor their ability to form charged species have an impact on the ELSD response. This detector is consequently well adapted to compounds with weak chromophores and without available standards, such as the saponins of S. officinalis and C. officinalis.…”

Section: Discussionmentioning

confidence: 99%

“…The calibration curves were plotted after linear regression of the peak areas vs. concentrations in log base 10 units. The matrix effect was briefly investigated by comparing the estimated concentrations for compounds 25,27,29,31, and 33 when injecting the extract of the S. officinalis root at 5 and 10 mg/mL.…”

Section: Plants and Reagentsmentioning

confidence: 99%

Potential of extracts from Saponaria officinalis and Calendula officinalis to modulate in vitro rumen fermentation with respect to their content in saponins

Budan

Bellenot²,

Freuze

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Saponins have the potential to favorably modulate rumen fermentation, but there is generally a lack of the chemical structures associated with the described effects. The activity of extracts from Calendula officinalis and Saponaria officinalis in the rumen was evaluated in vitro. The S. officinalis root extract, reduced CH4 production by 8.5% and increased total VFA concentration by 25.2%. C. officinalis and S. officinalis root extracts and the S. officinalis aerial part extract decreased the acetate to propionate ratio from 8.6 to 17.4%, according to the extract. An HPLC-ELSD analysis indicated that the saponin content ranged from 43.6 to 57.6 mg/g of dry matter (DM) in the C. officinalis extracts and from 224.0 to 693.8 mg/g of DM in the S. officinalis extracts, expressed as the hederacoside C equivalent. Identification of the saponin compounds present in the extracts by HPLC–MSn suggested that the saponin profile modulated the biological activities, showing the importance of determining the structure of saponins when evaluating extracts.

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High-performance liquid chromatography–mass spectrometry and evaporative light-scattering detector to compare phenolic profiles of muscadine grapes

Cited by 27 publications

References 32 publications

Off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry to improve coverage in lipidomics analysis

Off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry to improve coverage in lipidomics analysis

HPLC-qTOF-MS/MS-Based Profiling of Flavan-3-ols and Dimeric Proanthocyanidins in Berries of Two Muscadine Grape Hybrids FLH 13-11 and FLH 17-66

Potential of extracts from Saponaria officinalis and Calendula officinalis to modulate in vitro rumen fermentation with respect to their content in saponins

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