Encyclopedia of Analytical Chemistry 2008
DOI: 10.1002/9780470027318.a1618.pub2
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High‐Performance Liquid Chromatography/Mass Spectrometry in Peptide and Protein Analysis

Abstract: Since the 1970s, high‐performance liquid chromatography/mass spectrometry (HPLC/MS) has gone through many stages of development. The main technical challenge has been the coupling of liquid chromatography (LC) systems with mass spectrometers. Soft ionization techniques that have contributed to HPLC/MS for peptide and protein study include fast atom bombardment (FAB), matrix‐assisted laser desorption/ionization (MALDI), and electrospray ionization (ESI). Among these techniques, FAB was developed first and used … Show more

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