High-performance liquid chromatography method for the simultaneous determination of sulfamethoxazole and trimethoprim in bovine milk using an on-line clean-up column

Pereira, Airton Vicente; Cass, Quézia B.

doi:10.1016/j.jchromb.2005.08.006

Cited by 64 publications

(38 citation statements)

References 13 publications

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“…Nevertheless, for official control analysis of feed samples, LC coupled with UV detection represents a valid alternative, providing good results in terms of selectivity, sensitivity, instrument cost, and simplicity. To the best of our knowledge, there are a limited number of papers about determination of sulfonamides by LC coupled with UV-diode array detection [25][26][27][28][29][30]. These methods are applied to analysis of matrices different from animal feeds and do not allow simultaneous determination of several sulfonamides in feeds.…”

Section: Introductionmentioning

confidence: 99%

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

et al. 2011

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A simple, rapid, and reliable multiresidue method, based on liquid chromatography (LC) and ultraviolet (UV)-diode array detection (DAD), is described for assaying ten sulfonamides (sulfadiazine, sulfathiazole, sulfamerazine, sulfamethazine, sulfamethoxypyridazine, sulfamonomethoxine, sulfachloropyridazine, sulfamethoxazole, sulfaquinoxaline, and sulfadimethoxine) in feeds. The chromatographic separation is accomplished using a C18 column, eluted with a mobile phase consisting of acetate buffer, acetonitrile, and methanol. The sample preparation requires a simple extraction with chloroform/ acetone and a purification step by solid-phase extraction. The analytical parameters of precision, detection and quantification limits, recovery, and ruggedness have been evaluated by a validation procedure following the European guidelines of Regulation 882/2004/EC and Decision 657/2002/EC.

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Section: Introductionmentioning

confidence: 99%

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

et al. 2011

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“…The chemical outer layer of SPS physically excludes unwanted molecules, but also provides selectivity by chemical mechanisms through different compositions of the outer layer [37,79,80]. The first initial SPS materials were developed based on the incorporation of two phases, an inner and an outer phase, which are independently bound to the exterior surface of a silica gel particle.…”

Section: Sps Phase Trap Columnsmentioning

confidence: 99%

Restricted access media as a streamlined approach toward on‐line sample preparation: Recent advancements and applications

Yang

Fan

Classon³

et al. 2013

J of Separation Science

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Restricted access media (RAM) as an alternative to traditional sample preparation strategies are reviewed. RAM comprise chromatographic packing materials that combine, typically, a restrictive outer surface to exclude the retention of large biomolecules, which are common interferences in biological fluids, with retentive inner pores or phases to capture analytes of interest. Through the years, a variety of RAM formats have been created, including internal surface phases, semipermeable phases, and molecularly imprinted polymer phases. Many phases are commercially available through a variety of manufacturers. The use of on-line sample preparation using RAM can increase throughput, recovery, and ease of use for sample preparation of complex biological matrices. The state-of-the-art with respect to production and use of these media for a variety of applications is covered.

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“…[2] In consequence, residues of sulfonamides can be found in animal tissues, milk, and also in environmental samples such as surface waters causing adverse effects in humans such as allergic reactions in hypersensitive people besides to induce high levels of bacterial resistance. [3][4][5][6] The sulfamethoxazole (SMX) is a sulfonamide commonly administered in animals and humans. As a result, several methodologies for the analysis of SMX residues have been developed, including spectroscopic, [7] electrochemical, [8] or separation techniques based on capillary electrophoresis [9] and highperformance liquid chromatography.…”

Section: Introductionmentioning

confidence: 99%

Dispersive solid-phase extraction based on butylamide silica for the determination of sulfamethoxazole in milk samples by capillary electrophoresis

Islas

Rodríguez

Páez-Hernández

et al. 2016

Journal of Liquid Chromatography & Related Technologies

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A new method based on the combination of dispersive solid-phase extraction and capillary electrophoresis is proposed for the determination of sulfamethoxazole in milk samples. Butylamide silica was synthesized and used as extractant. Factors involved in sample treatment method such as: butylamide silica amount, NaOH concentration in methanol, sample volume, and dispersion time were evaluated using a Taguchi parameter design. Under optimal conditions, average recoveries ranged from 73 to 85% with a limit of detection of 0.05 mg L À 1 were achieved. The proposed method is a useful technique for cleanup milk samples. GRAPHICAL ABSTRACT

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High-performance liquid chromatography method for the simultaneous determination of sulfamethoxazole and trimethoprim in bovine milk using an on-line clean-up column

Cited by 64 publications

References 13 publications

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

Restricted access media as a streamlined approach toward on‐line sample preparation: Recent advancements and applications

Dispersive solid-phase extraction based on butylamide silica for the determination of sulfamethoxazole in milk samples by capillary electrophoresis

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