High-performance liquid chromatography of proteins on stabilized polymer-diol-bonded silica-gel stationary phase

Szabó, Gyula; Offenmüller, Katalin; Csató, Edit

doi:10.1021/ac00154a005

Cited by 7 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…291 Diol-bonded Si-gel has been used as a stationary phase for the separation of peptides and proteins. [292][293][294] The chromatographic behavior of peptidic substances was determined either by the pH of the eluent, the stationary phase presenting a net negative charge in buffered solutions, 292,293 or as a result of size-exclusion, also in buffer. 294 Size-exclusion chromatography on diol-bonded Si-gel was also observed for synthetic polymers in reversed-phase mode.…”

Section: Ion-exchange Chromatographymentioning

confidence: 99%

“…[292][293][294] The chromatographic behavior of peptidic substances was determined either by the pH of the eluent, the stationary phase presenting a net negative charge in buffered solutions, 292,293 or as a result of size-exclusion, also in buffer. 294 Size-exclusion chromatography on diol-bonded Si-gel was also observed for synthetic polymers in reversed-phase mode. 295 For low molecular weight aromatic analytes, dipolar interactions determined the chromatographic behavior on diol-bonded Sigel by normal-phase chromatography.…”

Section: Ion-exchange Chromatographymentioning

confidence: 99%

See 1 more Smart Citation

The isolation of water-soluble natural products – challenges, strategies and perspectives

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Ion-exchange Chromatographymentioning

confidence: 99%

Section: Ion-exchange Chromatographymentioning

confidence: 99%

The isolation of water-soluble natural products – challenges, strategies and perspectives

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The emergence of new packing materials for SEC has changed this perception (12)(13)(14). Modern SEC packing materials are typically rigid, 4-10 pm, silica-based spheres that have controlled pore sizes.…”

Section: J Microcolumn Sepcrrutionsmentioning

confidence: 99%

Efficiency of packed microcolumns compared with large‐bore packed columns in size‐exclusion chromatography

Kennedy

Jorgenson

1990

J Microcolumn Separations

View full text Add to dashboard Cite

Abstract.Columns with inner diameters of 50 and 28 pm were slurry-packed with 4-5 pm size-exclusion chromatography particles (Du Pont GF 250 Zorbax Bioseries) and compared with a commercially prepared 9.4-mm i.d. column that was packed with the Same particles. The columns were compared on the basis of efficiencies, peak shapes, and van Deemter coefficients obtained for three proteins used as test analytes. The microcolumns provided more symmetrical peak shapes and lower plate heighls than those provided by the commercial column if the peaks were analyzed using statistical moments. For example, h,,, for bovine serum albumin was 5.6 in the commercial column and 2.1 in the microcolumn. This difference is attributed to the quality of the packing process, based on the fact that the van Deemter A term was significantly lower for the microcolumn than for the conventional column. The van Deemter data demonstrate that the A term was the major source of band broadening in the commercial column throughout the range of velocities used, and both theA and C terms were significant for the microcolumn. A 90 cm X 50 pm column was prepared and showed approximately 80% of the expected increase in efficiency.

show abstract

Quality Control of Albumin Solutions by Size-Exclusion High-Performance Liquid Chromatography, Isoelectric Focusing, and Two-Dimensional Immunoelectrophoresis

Liedekerke¹,

Nelis²,

Kint³

et al. 1991

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

High-performance liquid chromatography of proteins on stabilized polymer-diol-bonded silica-gel stationary phase

Cited by 7 publications

References 12 publications

The isolation of water-soluble natural products – challenges, strategies and perspectives

The isolation of water-soluble natural products – challenges, strategies and perspectives

Efficiency of packed microcolumns compared with large‐bore packed columns in size‐exclusion chromatography

Quality Control of Albumin Solutions by Size-Exclusion High-Performance Liquid Chromatography, Isoelectric Focusing, and Two-Dimensional Immunoelectrophoresis

Contact Info

Product

Resources

About