High-performance liquid chromatography of thiols and disulfides: Dinitrophenol derivatives

Fariss, Marc W.; Reed, Donald J.

doi:10.1016/0076-6879(87)43018-8

Cited by 483 publications

(219 citation statements)

References 6 publications

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“…The content of GSH and GSSG was determined according to Fariss and Reed (1987). Frozen tissue was ground in liquid nitrogen and homogenised in 1:10 (w/v) pre-cooled PCA (10% containing 2 mM bathophenanthroline-disulfonic acid) bubbled with nitrogen.…”

Section: Determination Of Reduced (Gsh) and Oxidized (Gssg) Glutathiomentioning

confidence: 99%

Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet Nacella concinna

Weihe

Kriews

Abele

2010

Marine Environmental Research

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Section: Determination Of Reduced (Gsh) and Oxidized (Gssg) Glutathiomentioning

confidence: 99%

Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet Nacella concinna

Weihe

Kriews

Abele

2010

Marine Environmental Research

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“…Plasma total homocysteine and cysteine concentrations were analysed according to the reverse phase-HPLC method of Araki and Sako (1987), with modifications as suggested by Gilfix et al (1997). For liver thiol concentrations, extracts were first prepared as described earlier (Farris and Reed, 1987), and subsequently treated as described for plasma (Araki and Sako, 1987;Gilfix et al, 1997). Concentrations of total homocysteine, cysteine and hepatic PLP were determined through the use of an external standard curve, and the inter-and intra-assay coefficient of variation (CV) were ,2%.…”

Section: Animals and Feedingmentioning

confidence: 99%

Impairments in pyridoxine-dependent sulphur amino acid metabolism are highly sensitive to the degree of vitamin B6 deficiency and repletion in the pig

Zhang

Kebreab

Jing

et al. 2009

Animal

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The objectives of the current study included the characterization of the temporal changes in indices of sulphur amino acid metabolism in piglets in response to vitamin B 6 deficiency and repletion with graded levels of pyridoxine hydrochloride. In Experiment 1, 12 piglets (average initial weight 5 5.3 kg; n 5 6 per group) were fed a semi-purified diet containing either 0 (deficiency group) or 3 mg (control group) pyridoxine Á HCl/kg diet, using a pair-feeding design, for 6 weeks. Piglets consuming vitamin B 6 -deficient diets exhibited decreased average daily gains on the 4th week and feed conversion efficiency from the 4th week until the end of the trial ( P , 0.05). Plasma pyridoxal-5 0 -phosphate (PLP), in pigs consuming vitamin B 6 -deficient diets, was significantly lower than controls throughout the experiment ( P , 0.01), reaching a nadir of 14% of the control animals' value by the end of the trial. Indices of sulphur amino acid metabolism, including activities of hepatic cystathionine-b-synthase (CBS), cystathionine-g-lyase (CGL) and serine hydroxymethyltransferase, as well as hepatic-free cysteine concentrations were markedly decreased after 6 weeks of B 6 deficiency ( P , 0.05). Total hepatic mRNA expressions for CBS and CGL were not affected. Concurrently, hepatic-free homocysteine concentrations increased by more than eight-fold ( P , 0.01) at the end of the trial. An examination of plasma total homocysteine and cysteine concentrations revealed significant ( P , 0.05) differences between treatments, with evidence of an abrupt shift in concentrations at 3 weeks post-initiation of dietary treatments (.25-fold increase in homocysteine; halving of cysteine values). At the end of Experiment 1, vitamin B 6 deficiency significantly increased plasma methionine and serine levels, but decreased plasma glycine concentrations ( P , 0.05). In Experiment 2, 20 pigs of 14 days old (initial BW 5 5.0 kg) were subjected to a 4-week vitamin B 6 depletion protocol, based on results obtained in Experiment 1. After the depletion period and assessment of baseline status (four pigs), remaining pigs were allocated to one of four dietary vitamin B 6 repletion treatments: 0.75, 1.5, 2.25 and 3 mg/kg diet as pyridoxine Á HCl (n 5 4 per level) for 14 days. Significant dose-dependent increases in plasma PLP and cysteine, and decreases in homocysteine were observed, and these were sensitive to the duration of repletion. In conclusion, data from the current studies support the use of both plasma PLP and homocysteine as sensitive indices of vitamin B 6 status in the pig. Additionally, the observed patterns of responses in vitamin B 6 -sensitive metabolites are supportive of an inclusion level of 2.25 mg/kg diet, as pyridoxine Á HCl, in diets for young pigs.Keywords: cysteine, homocysteine, pig, sulphur amino acid, vitamin B 6 ImplicationsThe current study has provided a temporal characterization of the impact of B 6 deficiency and subsequent repletion on indices of sulphur amino acid metabolism in young pigs. The use of plasma and h...

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“…Reduced (GSH) and oxidised (GSSG) glutathione measured according to Fariss and Reed (1987) and calculation of cellular redox potential…”

Section: Oxidative Stress Parametersmentioning

confidence: 99%

Effects of seasonal and latitudinal cold on oxidative stress parameters and activation of hypoxia inducible factor (HIF-1) in zoarcid fish

Heise

Estevez²,

Puntarulo³

et al. 2007

J Comp Physiol B

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Acute, short term cooling of North Sea eelpout Zoarces viviparus is associated with a reduction of tissue redox state and activation of hypoxia inducible factor (HIF-1) in the liver. The present study explores the response of HIF-1 to seasonal cold in Zoarces viviparus, and to latitudinal cold by comparing the eurythermal North Sea fish to stenothermal Antarctic eelpout (Pachycara brachycephalum). Hypoxic signalling (HIF-1 DNA binding activity) was studied in liver of summer and winter North Sea eelpout as well as of Antarctic eelpout at habitat temperature of 0°C and after long-term warming to 5°C. Biochemical parameters like tissue iron content, glutathione redox ratio, and oxidative stress indicators were analyzed to see whether the cellular redox state or reactive oxygen species formation and HIF activation in the fish correlate. HIF-1 DNA binding activity was significantly higher at cold temperature, both in the interspecific comparison, polar vs. temperate species, and when comparing winter and summer North Sea eelpout. Compared at the low acclimation temperatures (0°C for the polar and 6°C for the temperate eelpout) the polar fish showed lower levels of lipid peroxidation although the liver microsomal fraction turned out to be more susceptible to lipid radical formation. The level of radical scavenger, glutathione, was twofold higher in polar than in North Sea eelpout and also oxidised to over 50%. Under both conditions of cold exposure, latitudinal cold in the Antarctic and seasonal cold in the North Sea eelpout, the glutathione redox ratio was more oxidised when compared to the warmer condition. However, oxidative damage parameters (protein carbonyls and thiobarbituric acid reactive substances (TBARS) were elevated only during seasonal cold exposure in Z. viviparus. Obviously, Antarctic eelpout are keeping oxidative defence mechanisms high enough to avoid accumulation of oxidative damage products at low habitat temperature. The paper discusses how HIF could be instrumental in cold adaptation in fish.

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High-performance liquid chromatography of thiols and disulfides: Dinitrophenol derivatives

Cited by 483 publications

References 6 publications

Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet Nacella concinna

Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet Nacella concinna

Impairments in pyridoxine-dependent sulphur amino acid metabolism are highly sensitive to the degree of vitamin B6 deficiency and repletion in the pig

Effects of seasonal and latitudinal cold on oxidative stress parameters and activation of hypoxia inducible factor (HIF-1) in zoarcid fish

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