High-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (HPLC-QTOFMS) Analysis on the Ethanol:Water (80:20) Extract of Lawsonia inermis Leaves

Othman, Muhammad Remy; Othman, Rozana; Ismail, Aziah; Hazni, Hazrina; Ahmad, Khadher; Razzak, Munirah Abd; Yusoff, Zulkifli Mohd; Awang, Khalijah

doi:10.17576/jsm-2020-4907-12

Cited by 3 publications

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“…Compound 12 was identified as niazinin B as it yielded a parent ion peak at m/z 342.1115 [M-H]-, generating characteristic ion peaks at m/z 310.07 and 196.04 resulting from the loss of MeOH and the rhamnosyl moiety (−146 Da), respectively [ 19 ] ( Figure 1 C). Compound 13 produced a parent ion peak [M-H] − at m/z 345.0822, yielding characteristic fragment ion peaks at m/z 301.05 and 183.02 resulting from the loss of CH 3 CHO (−44 Da) and a glucose moiety (−162 Da), respectively, and was confirmed as lalioside [ 20 ] ( Figure 1 D). Compound 14 had a precursor ion peak [M-H] − at m/z 353.0865, generating daughter ion peaks at m/z 309.09 (loss of CO 2 ), m/z 191.05 (loss of caffeic moiety), m/z 179.03 (loss of quinic moiety), and m/z 173.04 (dehydrated quinic moiety) and was characterized as 5- O -caffeoylquinic acid [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

High Resolution Mass Spectroscopy-Based Secondary Metabolite Profiling of Nymphaea nouchali (Burm. f) Stem Attenuates Oxidative Stress via Regulation of MAPK/Nrf2/HO-1/ROS Pathway

et al. 2021

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The secondary metabolites profiling of Nymphaea nouchali stem (NNSE) extract was carried out using a high-resolution mass spectroscopic technique. The antioxidant effects of NNSE, as well as the underlying mechanisms, were also investigated in tert-butyl hydroperoxide (t-BHP)-stimulated oxidative stress in RAW264.7 cells. Tandem mass spectroscopy with (−) negative mode tentatively revealed the presence of 54 secondary metabolites in NNSE. Among them, phenolic acids and flavonoids were predominant. Phenolic acids (brevifolincarboxylic acid, p-coumaroyltartaric acid, niazinin B, lalioside, 3-feruloylquinic acid, and gallic acid-O-rutinoside), flavonoids (elephantorrhizol, apigenin-6-C-galactoside 8-C-arabinoside, and vicenin-2), sialic acid (2-deoxy-2,3-dehydro-N-acetylneuraminic acid), and terpenoid (α-γ-onoceradienedione) were identified in NNSE for the first time. Unbridled reactive oxygen species/nitrogen species (ROS/RNS) and redox imbalances participate in the induction and development of many oxidative stress-linked diseases. The NNSE exhibited significant free radical scavenging capabilities and was also able to reduce t-BHP-induced cellular generation in RAW264.7 cells. The NNSE prevented oxidative stress by inducing the endogenous antioxidant system and the levels of heme oxygenase-1 (HO-1) by upregulating Nrf2 through the modulation of mitogen-activated protein kinases (MAPK), such as phosphorylated p38 and c-Jun N terminal kinase. Collectively, these results indicate that the NNSE exhibits potent effects in preventing oxidative stress-stimulated diseases and disorders through the modulation of the MAPK/Nrf2/HO-1 signaling pathway. Our findings provide new insights into the cytoprotective effects and mechanisms of Nymphaea nouchali stem extract against oxidative stress, which may be a useful remedy for oxidative stress-induced disorders.

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Section: Resultsmentioning

confidence: 99%