High‐performance liquid chromatography study of gatifloxacin and sparfloxacin using erythrosine as post‐column resonance Rayleigh scattering reagent and mechanism study

Abstract

Herein, a highly selective high-performance liquid chromatography (HPLC) coupled with resonance Rayleigh scattering (RRS) method was developed to detect gatifloxacin (GFLX) and sparfloxacin (SPLX). GFLX and SPLX were first separated by HPLC, then, in pH 4.4 Britton-Robinson (BR) buffer medium, protonic quaternary ammonia cation of GFLX and SPLX reacted with erythrosine (ERY) to form 1:1 ion-association complexes, which resulted in a significant enhancement of RRS signal. The experimental conditions of HPLC and post-column RRS have been investigated, including detection wavelength, flow rate, pH, reacting tube length and reaction temperature. Reaction mechanism were studied in detail by calculating the distribution fraction. The maximum RRS signals for GFLX and SPLX were recorded at λ = λ = 330 nm. The detection limits were 3.8 ng ml for GFLX and 17.5 ng ml for SPLX at a signal-to-noise ratio of 3. The developed method was successfully applied to the determination of GFLX and SPLX in water samples. Recoveries from spiked water samples were 97.56-98.85%.

Xi Lei San Attenuates Dextran Sulfate Sodium-Induced Colitis in Rats and TNF-α-Stimulated Colitis in CACO2 Cells: Involvement of the NLRP3 Inflammasome and Autophagy

“…As in previous studies [24,25], the chromatograms of Xi Lei San and indirubin were confirmed by HPLC. Xi Lei San and indirubin solutions were prepared and analyzed by HPLC performed using a Hypersil NH2 column (250 mm × 4:6 mm, 5 mm).…”

Quantitative evaluation and chromatographic fingerprinting for the quality assessment of Pudilan tablet

“…This technique has been applied by the World Health Organization (WHO), the State Food and Drug Administration of China (SFDA) as a strategic plan for the quality assessment of TCM [9,10], and got increasing concerns because it focuses on the characterization of the total sample composition. Several ways, for instance, high-performance thin layer chromatography (HPTLC) [11][12][13][14], thin-layer chromatography (TLC) [15][16][17], infrared spectroscopy (IR) [18][19][20], mass spectrometry (MS) [21,22], gas chromatography (GC) [23,24], high-performance liquid chromatography (HPLC) [25][26][27], nuclear magnetic resonance (NMR) [28][29][30][31] and high-performance liquid chromatography (UPLC) [32][33][34] are utilized for fingerprinting to characterize multiple compounds in the tested herbal samples. Among the abovementioned methods, HPLC is generally the most applied method for its high sensitivity, selectivity, and low cost [35].…”

Applying resonance Rayleigh scattering and spectrofluorimetric techniques for the selective determination of neomycin sulfate using Congo red as a probe: Applications for wastewater analysis

“…To date, the RRS method has been widely used for the determination of trace substances, for example, nucleic acids, [ 28 ] proteins, [ 29 ] inorganic ions, [ 30,31 ] organic compounds, [ 32,33 ] drugs, [ 34–36 ] etc. [ 37–39 ] Recent studies have shown that the RRS method can be also applied to the determination of antibiotics such as fluoroquinolones, [ 40,41 ] cephalosporins, [ 42 ] tetracyclines, [ 43 ] macrolides, [ 44 ] aminoglycosides, [ 45 ] etc. In the past few years, there have been reports on the quantitative analysis of NEO using the RRS method.…”