High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples

Hows, Mark E. P.; Lacroix, Laurent; Heidbreder, Christian; Organ, Andrew J.; Shah, Ajit J.

doi:10.1016/j.jneumeth.2004.03.021

Cited by 185 publications

(102 citation statements)

References 18 publications

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“…A variety of methods have been developed to measure serotonin including spectrophotometry [21,22], LCfluorometry [23,24], enzyme immunoassay, and LCelectrochemical detection [25,26]. LC-mass spectrometric methods is however the method of choice as tandem LC-MS methods provide increased selectivity while reducing the need for dependence on chromatographic separation [27][28][29][30][31][32][33][34][35]. While these methods report good results for the analytes under investigation, the methods do not facilitate multi-target analysis which was the aim of this study.…”

Section: Introductionmentioning

confidence: 99%

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

et al. 2011

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Serotonin is a major neurotransmitter and affects various functions both in the brain and in the rest of the body. It has been demonstrated that altered serotinergic function is implicated in various psychiatric disorders including depression and schizophrenia. Serotonin has also been implicated along with dopamine in attention deficithyperkinetic disorder (AD-HKD). This study provides a versatile validated method for the analysis of serotonin, hydroxyindole acetic acid and dopamine in urine using LC-MS/MS. This method was then used to quantify these analytes in a test group of 17 children diagnosed with severe AD-HKD. This group was compared to a matched control group to investigate the possibility that one of these compounds may be a potential biomarker for this condition. The developed method provided good linear calibration curves for the multiplex assay of analytes in urine (0.05-3.27 nmol/L; R 2 ≥0.9977). Acceptable inter-day repeatability was achieved for all analytes with RSD values (n=9) ranging from 1.1% to 9.3% over a concentration range of 0.11-3.27 μmol/L in urine. Excellent limits of detection (LOD) and limits of quantitation (LOQ) were achieved with LODs of 8.8-18.2 nmol/L and the LOQs of 29.4-55.7 nmol/L for analytes in urine. Recoveries were in the ranges of 98-104%, 100-106% and 91-107% for serotonin, 5-HIAA and dopamine, respectively. An appropriate sample clean-up procedure for urine was developed to ensure efficient recovery and reproducibility on analysis. Evaluation of matrix effects was also carried out and the influence of ion suppression on analytical results reported. Confirmatory analysis was carried out on a linear trap quadrupole-Orbitrap mass spectrometer to obtain high mass accuracy data of the target analytes in the clinical samples.

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Section: Introductionmentioning

confidence: 99%

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

et al. 2011

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“…8 Various commonly usable analytical methods for DA and its analogs have been developed in the past. Some examples of these methods are rapid liquid chromatography/ tandem mass spectrometry (LC-MS/MS), 9 the chromatography method, [10][11][12][13] and the capillary electrophoresis mass spectrometry method. 14 These methods are very sensitive.…”

Section: Introductionmentioning

confidence: 99%

Zeolite Nanoparticle Modified Carbon Paste Electrode as a Biosensor for Simultaneous Determination of Dopamine and Tryptophan

Khalilzadeh

Hasanzadeh

Bahareh

et al. 2009

J Chinese Chemical Soc

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A new chemically modified electrode is constructed based on an iron(III) doped zeolite modified carbon paste electrode (Fe 3+ Y/ZCME). The electrode was evaluated as a sensor for sub-micromolar determination of tryptophan (Trp) and dopamine (DA). The measurements were carried out using the differential pulse voltammetry (DPV) method in a phosphate buffer solution with pH = 5. The prepared modified electrode shows voltametric responses with high sensitivity and stability for DA and Trp in optimal conditions. The analytical performance of this sensor has been evaluated for detection of DA and Trp in human serum.

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“…Liquid chromatography online mass spectrometry (LC-MS) allows selective identification and sensitive quantification of a great number of molecules. Although LC-MS methods have been developed for the identification and quantification in microdialysates of neurotransmitters [8][9][10] , neuropeptides [11] and exogenous compounds such as drugs and their metabolites [12][13][14] , most studies have been restricted to a small number of analytes and consequently have resulted in only narrow insights in pathophysiological mechanisms.…”

Section: Introductionmentioning

confidence: 99%

NextGen Brain Microdialysis: Applying Modern Metabolomics Technology to the Analysis of Extracellular Fluid in the Central Nervous System

et al. 2015

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olomics platform was applied to the analysis of microdialysates in a foot shock-induced mouse model of posttraumatic stress disorder (PTSD). The rich metabolite data information was then used to delineate affected prefrontal molecular pathways that reflect individual susceptibility for developing PTSD-like symptoms. We demonstrate that hypothesis-free metabolomics can be adapted to the analysis of microdialysates for the discovery of small molecules with functional significance.

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High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples

Cited by 185 publications

References 18 publications

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

Zeolite Nanoparticle Modified Carbon Paste Electrode as a Biosensor for Simultaneous Determination of Dopamine and Tryptophan

NextGen Brain Microdialysis: Applying Modern Metabolomics Technology to the Analysis of Extracellular Fluid in the Central Nervous System

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