High-performance liquid chromatography with fluorescence detection and ultra-performance liquid chromatography with electrospray tandem mass spectrometry method for the determination of indoleamine neurotransmitters and their metabolites in sea lamprey plasma

Wang, Jianji; Walaszczyk, Erin J.; Li, Ké; Chung-Davidson, Yu Wen; Li, Weiming

doi:10.1016/j.aca.2012.01.025

Cited by 43 publications

(25 citation statements)

References 49 publications

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“…Numerous LC/MRM/MS methods have been developed for the analysis of NTs from various biological fluids. Analyses of NTs from sea lamprey brain and plasma, from human urine, blood and brain of the rhesus monkey, from rat brain, from human plasma, etc., have been reported in the literature . In all those methods only few numbers of targeted NTs were quantified.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of neurotransmitters from regenerating planarian extract using an ultrahigh‐performance liquid chromatography/mass spectrometry/selected reaction monitoring method

Rangiah

Palakodeti

2013

Rapid Comm Mass Spectrometry

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Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of neurotransmitters from regenerating planarian extract using an ultrahigh‐performance liquid chromatography/mass spectrometry/selected reaction monitoring method

Rangiah

Palakodeti

2013

Rapid Comm Mass Spectrometry

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“…Mobile phase A consisted of 0.1% formic acid in water, and mobile phase B was acetonitrile with 0.1% formic acid. Separation was achieved using a gradient program at a flow rate of 0.4 mL/min. The capillary voltage and extractor voltage were 2.5 kV and 4 V, respectively; the flow rate of the cone gas and desolvation gas was 150 and 800 L/hr, respectively, and the source temperature and desolvation temperatures were 150 and 350°C, respectively.…”

Section: Methodsmentioning

confidence: 99%

Melatonin blunts the mitochondrial/NLRP3 connection and protects against radiation‐induced oral mucositis

Ortiz

Acuña-Castroviejo

Doerrier

et al. 2014

Journal of Pineal Research

130

128

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Mucositis is a common and distressing side effect of chemotherapy or radiotherapy that has potentially severe consequences, and no treatment is available. The purpose of this study was to analyze the molecular pathways involved in the development of oral mucositis and to evaluate whether melatonin can prevent this pathology. The tongue of male Wistar rats was subjected to irradiation (X-ray YXLON Y.Tu 320-D03 irradiator; the animals received a dose of 7.5 Gy/day for 5 days). Rats were treated with 45 mg/day melatonin or vehicle for 21 days postirradiation, either by local application into their mouths (melatonin gel) or by subcutaneous injection. A connection between reactive oxygen species, generating mitochondria and the NLRP3 (NLR-related protein 3 nucleotide-binding domain leucine-rich repeat containing receptor-related protein 3) inflammasome, has been reported in mucositis. Here, we show that mitochondrial oxidative stress, bioenergetic impairment and subsequent NLRP3 inflammasome activation are involved in the development of oral mucositis after irradiation and that melatonin synthesized in the rat tongue is depleted after irradiation. The application of melatonin gel restores physiological melatonin levels in the tongue and prevents mucosal disruption and ulcer formation. Melatonin gel protects the mitochondria from radiation damage and blunts the NF-κB/NLRP3 inflammasome signaling activation in the tongue. Our results suggest new molecular pathways involved in radiotherapy-induced mucositis that are inhibited by topical melatonin application, suggesting a potential preventive therapy for mucositis in patients with cancer.

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“…Crude HPLC-FLD data are given in the Online Supplementary Appendix. The HPLC-FLD method has been validated in terms of linearity, precision and accuracy, recovery, matrix effect, reproducibility of retention time and stability [22], and the method is known to be simple, accurate and suitable for routine analysis. [23] Moreover, it allows complete purification of CAs by an organic and specific complexing reagent and allows having a complete extraction using a C-18 solid phase column.…”

Section: Resultsmentioning

confidence: 99%

Catecholamine levels in a Ramadan fasting model in rats: a case control study

Bucaktepe

Akdağ

Daşdağ

et al. 2016

Biotechnology & Biotechnological Equipment

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Eating habits as well as physical exercise are very important for a healthy lifespan. Ramadan-type fasting, which is food and water avoidance during the daylight period for four weeks, has drawn attention due to its positive impacts on metabolism and health. The aim of this study was to compare the blood and urine catecholamine (CA) levels in fasting and non-fasting rats, in terms of stress response. A total of 20 male rats were randomly divided into a fasting group and a control group. Four weeks later, blood and urine samples were taken after decapitation. Analysis of CAs was done using high-performance liquid chromatography with florescence detection (HPLC-FLD). The dopamine (DA), adrenaline (ADR) and noradrenaline (NA) blood and urine concentrations were found to be higher in the fasting group compared to the control group, but the difference was statistically significant only for the blood DA levels (p < 0.05). In the fasting group, the blood values of ADR and NA correlated with each other but not with the DA levels, whereas there was correlation among the urine levels of DA, ADR and NA. In the control group, the blood and urine values of DA, ADR and NA correlated with each other. The differences observed in the blood and urine CAs indicate a specific regulation of CAs in Ramadan-type fasting, which needs to be investigated thoroughly in future studies.

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High-performance liquid chromatography with fluorescence detection and ultra-performance liquid chromatography with electrospray tandem mass spectrometry method for the determination of indoleamine neurotransmitters and their metabolites in sea lamprey plasma

Cited by 43 publications

References 49 publications

Comprehensive analysis of neurotransmitters from regenerating planarian extract using an ultrahigh‐performance liquid chromatography/mass spectrometry/selected reaction monitoring method

Comprehensive analysis of neurotransmitters from regenerating planarian extract using an ultrahigh‐performance liquid chromatography/mass spectrometry/selected reaction monitoring method

Melatonin blunts the mitochondrial/NLRP3 connection and protects against radiation‐induced oral mucositis

Catecholamine levels in a Ramadan fasting model in rats: a case control study

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