High-performance liquid chromatography with multi-wavelength detection of the bisphenol A impurities

Poskrobko, J.; Dejnega, M.; Kiedik, M

doi:10.1016/s0021-9673(00)00418-0

Cited by 25 publications

(6 citation statements)

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“…We could determine phenols in serum sample compared to previous CZE (Gaitonde and Pathak, 1990) and MEKC (Takeda et al, 2000). It was proved that the proposed MEKC method could be an alternative method for the determination of bisphenol A and alkyl phenols in serum compared with GC-MS (Mol et al, 2000) and LC (Sajiki et al, 1999;Poskrobko et al, 2000;Pedersen and Lindholst, 1999).…”

Section: Calibration Graphsmentioning

confidence: 99%

“…Gas chromatography-mass spectrometry (GC-MS; Mol et al, 2000) and high performance liquid chromatography (HPLC) equipped with photometric detector or fluorimetric detector (Sajiki et al, 1999;Poskrobko et al, 2000;Pedersen and Lindholst, 1999) have been reported for analysis of bisphenol A and alkylphenols. Capillary electrophoresis (CE) has been reported as a new analytical method in recent years.…”

Section: Introductionmentioning

confidence: 99%

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Determination of bisphenol A and 10 alkylphenols in serum using SDS micelle capillary electrophoresis with γ‐cyclodextrin

Katayama

Matsuda

Sasaki

et al. 2001

Biomedical Chromatography

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A new micelle capillary electrophoresis based on cyclodextrin micellar electrokinetic chromatography (MEKC) for the determination of bisphenol A and 10 alkylphenols in rat serum is reported. Several surfactants and dextrins were studied. Bisphenol A and alkylphenols were separated using a 50 microm x 50 cm capillary with 20 mM borate phosphate buffer (pH 8.0) containing 20 mM sodium dodecylsulfate and 5 mM gamma-cyclodextrin as carrier. The method could determine 0.6-2000 microg/mL of phenols in 100 microL serum by photometric detection at 214 nm. Using 2.0 mL serum, 1.0 ng/mL of phenols could be determined. The relative standards deviations were 6.3-7.7% at 10 microg/mL in serum. The recoveries were 91.8-93.0% with 10 microg/mL serum samples.

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Section: Calibration Graphsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of bisphenol A and 10 alkylphenols in serum using SDS micelle capillary electrophoresis with γ‐cyclodextrin

Katayama

Matsuda

Sasaki

et al. 2001

Biomedical Chromatography

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“…BPA increased the expression of both AhR and Arnt mRNAs, suggesting that ER-mediated estrogenic effects may be promoted by BPA. Furthermore, the estrogenic activity of BPA is increased after co-incubation with the liver to c a t a b o l i z e t h e B P A , i n d i c a t i n g t h a t B P A metabolite(s) may have a higher estrogenic potency than the mother compound [49][50][51]. We suggest that BPA itself plays a key role in the estrogenic activity of mid-stage embryos because activity for drug-metabolism in embryonic livers is extremely weak.…”

Section: Discussionmentioning

confidence: 99%

Effects of Exposure <i>In Utero</i> to Bisphenol A on the Expression of Aryl Hydrocarbon Receptor, Related Factors, and Xenobiotic Metabolizing Enzymes in Murine Embryos

Nishizawa

Imanishi

Manabe

2005

Journal of Reproduction and Development

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Abstract.To evaluate the effects of bisphenol A (BPA), a candidate endocrine disruptor (ED), on embryonic development, we examined the mRNA expression levels of the aryl hydrocarbon receptor (AhR; which binds with many EDs and plays crucial roles in their metabolism) and related factors [aryl hydrocarbon receptor repressor (AhRR) and AhR nuclear translocator (Arnt)], xenobiotic metabolizing enzymes [XMEs; cytochrome P450 1A1 (CYP1A1) and UDP-glucuronosyltransferase, and the glutathione S-transferase Ya subunit (GST)], in murine embryos exposed in utero to BPA (0.02, 2, 200, and 20,000 µg/kg/day) and 17β-estradiol (E2; 5 µg/kg/day, used as a positive control) at 6.5-13.5 or 6.5-17.5 days post coitum (dpc) using the quantitative real-time reverse transcriptionpolymerase chain reaction (RT-PCR) method. Protein levels of CYP1A1 and GST in embryonic livers were estimated by Western immunoblotting. Exposure in utero to BPA [0.02 (1/100 dose of environmental exposure), 2, 200, and 20,000 µg/kg/day] increased AhR mRNA expression in the cerebra, cerebella, and gonads (testes and ovaries) of male and female mid-and late-developmental stage (14.5-and 18.5-dpc, respectively) embryos. BPA dose-independently up-regulated the expression of AhRR and Arnt in mid-and late-stage embryos. BPA had no remarkable effect on the mRNA levels of XMEs in mid-stage embryos, but dose-dependently up-regulated the expression in late-stage embryos. Moreover, the protein levels of these enzymes in the livers of late-stage embryos were increased. The present findings revealed that exposure to BPA in utero disrupts the expression of AhR and related factors and of xenobiotic metabolizing enzymes, and that mid-stage embryos, in the organogenic stage, are sensitive to BPA. Key words: Aryl hydrocarbon receptor (AhR), AhR relating factor, Bisphenol A (BPA), Murine embryo, Xenobiotic metabolizing enzymes (XMEs) (J. Reprod. Dev. 51: [593][594][595][596][597][598][599][600][601][602][603][604][605] 2005) isphenol A [BPA; 2,2-bis (4-hydroxyphenyl) propane], which is composed of two benzene rings and in conformation resembles a synthetic estrogen, diethylstilbestrol (DES), is a common plasticizer for polycarbonate and epoxy resins and a candidate environmental endocrine disruptor (ED) [1,2]. BPA has toxicological effects on the reproductive, immunological, and nervous systems in mammals, binds with estrogen receptor-α (ERα; NR3A1) and ER-β (NR3A2), and induces estrogenic activity [3][4][5][6]. BPA affects the expression levels of ERs and ER-like nuclear receptor mRNAs in the rat uterus [6], but the mechanism of its actions has yet

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“…Organic impurities in BPA raw material were analyzed by high performance liquid chromatography in an adaptation of the method described by Poskrobko et al,12 using a Waters 2695 liquid chromatograph equipped with a diode array detector system Waters 2996 (Waters, Milford, MA). The analysis were performed on an X‐Terra C18 column (i.d.…”

Section: Methodsmentioning

confidence: 99%

Experimental study of the influence of raw material impurities on yellowness index of transesterification polycarbonate

Godı́nez

Ríos

Hernández-Fernández

et al. 2010

J of Applied Polymer Sci

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This article covers a quantitative experimental study on the influence of raw materials and materials of construction impurities on polycarbonate color as expressed by a solution yellowness index. Major impurities in the production process were identified, synthesized, and spiked into the baseline materials. A fractional factorial experimental design was executed to provide some information of the influence of impurities as corresponding to levels similar to those occurring in the manufacturing practice. Analysis of the experimental design showed that isopropenylphenol (IPP), methoxyphenyl benzoate (MOB) and 9,9-dimethylxanthene (DMX) are the main producers of color bodies. A quantitative expression was derived which can be used to predict manufacturing problems with regards to color.

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High-performance liquid chromatography with multi-wavelength detection of the bisphenol A impurities

Cited by 25 publications

References 0 publications

Determination of bisphenol A and 10 alkylphenols in serum using SDS micelle capillary electrophoresis with γ‐cyclodextrin

Determination of bisphenol A and 10 alkylphenols in serum using SDS micelle capillary electrophoresis with γ‐cyclodextrin

Effects of Exposure <i>In Utero</i> to Bisphenol A on the Expression of Aryl Hydrocarbon Receptor, Related Factors, and Xenobiotic Metabolizing Enzymes in Murine Embryos

Experimental study of the influence of raw material impurities on yellowness index of transesterification polycarbonate

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