High-performance liquid chromatography with photodiode array detection and chemometrics method for the analysis of multiple components in the traditional Chinese medicine Shuanghuanglian oral liquid

Abstract

Shuanghuanlian oral liquid, a traditional Chinese medicine preparation, is a mixture of three herbs (Flos Lonicerae, Radix Scutellariae and Fructus Forsythiae). In this study, the quantitative analysis of three main active compounds, chlorogenic acid, forsythin and baicalin in samples from different manufacturers was performed rapidly by high-performance liquid chromatography coupled with photodiode array detection followed by Contour Projection coupled to stepwise regression treatment of the obtained three-dimensional spectra in which the partial overlap between adjacent target components existed. The method was validated for linearity (R>0.9940), precision (RSD<1.25%), recovery (92.20-102.50%), limit of detection (0.01-0.02 μg/mL) and limit of quantification (0.03-0.07 μg/mL). The results indicated that the combination of the three-dimensional spectra of traditional Chinese medicine and Contour Projection-stepwise regression offered an accurate, simple, low-cost and eco-friendly way for the rapid quantitative analysis of Shuanghuanlian oral liquid samples.

Aligning retention time shifts in HPLC three‐dimensional spectra by icoshift approach combined with data arrangement methods and the release of a graphical user interface

“…The employed data set was consisted of 24 mixture samples with the size of 101 (retention time points) × 27 (wavelength points) × 24 (sample number). The HPLC-PAD 3D spectrum of a sample and the contour projection plot [31] are shown in Figure 1A and B, respectively. [30].…”

“…Here, the contour projection plot [31] was applied to illustrate the consistency of the retention time of each compound in different samples before and after alignment. Here, the contour projection plot [31] was applied to illustrate the consistency of the retention time of each compound in different samples before and after alignment.…”

“…[30]. The HPLC-PAD 3D spectrum of a sample and the contour projection plot [31] are shown in Figure 1A and B, respectively. In Figure 1B, each value in axis was the maximum absorption intensity in all scanning wavelengths at each retention time point.…”

“…As demonstrated in Section 3.3, the retention time of corresponding compound in different samples should have a high degree of consistency after successful alignment. Here, the contour projection plot [31] was applied to illustrate the consistency of the retention time of each compound in different samples before and after alignment. As reported in ref.…”

Comprehensive metabolite profiling of Plantaginis Semen using ultra high performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry coupled with elevated energy technique

“…The metabolite profiles reveal chemical compositions of the herbal medicines and contribute to elucidate the pharmacodynamic substance. Various techniques such as photocolorimetry, spectrophotometry, HPLC, GC–MS, and LC–MS have been applied to analyze chemical constituents of herbal medicines . With recent advancements, UHPLC–ESI‐QTOF‐MS is considered as a powerful and reliable tool to identify compounds .…”