High-performance thin-layer chromatographic determination of ibuprofen in plasma

Save, Tanuja; Parmar, Deven; Devarajan, Padma V.

doi:10.1016/s0378-4347(96)00326-x

Cited by 21 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A literature survey has revealed that ibuprofen in combination with other drugs or in human plasma can be determined by using different analytical methods such as spectrophotometry, high-performance liquid chromatography (HPLC), and gas chromatography (GC) for pharmaceutical and clinical purposes [5][6][7][8][9][10][11][12][13][14]. A high performance thin-layer chromatographic method (HPTLC) for the quantification of ibuprofen from human plasma at limit of detection (LOD) 50 ng has been developed by Save and coworkers [15]. In 2005, Krzek et al developed a chromatographic-densitometric method for the identification and determination of both enantiomers of ibuprofen by means of reversed-phase thin-layer chromatographic plates and chiral mobile phase consisting of β-cyclodextrin [16].…”

Section: Introductionmentioning

confidence: 99%

Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

2020

View full text Add to dashboard Cite

Ibuprofen is one of the best-known nonsteroidal anti-inflammatory and analgesic drugs. Following the previous work, the current study is focused on estimating the effect of different chromatographic conditions on the sensitivity of thin-layer chromatography in combination with UV densitometry, i.e., the detection and quantification of ibuprofen in a wide range of its concentrations including the lowest limits of detection (LOD) and quantification (LOQ). For this purpose, a reliable and easy-to-use calculation procedure for LOD and LOQ determination is presented in this work. In addition, the impact of type plates and mobile phase composition on the LOD and LOQ, respectively, of this active substance is accurately described. The results of detection and the quantification level of ibuprofen obtained under applied chromatographic conditions confirmed the utility of silica gel plates as well as silica gel bonded phases (i.e., reversed-phase (RP) plates) in the thin-layer chromatography (TLC)-densitometric analysis of ibuprofen at a low level, i.e., from several nanograms (below one microgram) to a few micrograms/spot. Among all chromatographic systems tested, the best are those consisting of silica gel 60F254 plates with concentrating zone (1.05583) and the mixture of n-hexane:ethyl acetate:glacial acetic acid in ratio 36:12:2 (v/v/v) as well as RP plates, i.e., RP-18F254 aluminum plates developed with methanol:water in volume composition 40:10 (v/v). These chromatographic systems allowed quantifying ibuprofen in the amount of 0.229 µg/spot and 0.228 µg/spot, which is less than 1 µg/spot. It can be stated that different chromatographic systems in combination with UV densitometric scanning at 224 nm proposed in this work can be successfully applied for the cost-effective and sensitive determination of ibuprofen as a widely used drug component as well as a residual in domestic wastewater. It was found that the modification of silica gel as well as the layer thickness of unmodified or modified silica gel 60 can influence the quality of chromatograms and the detection/quantification of ibuprofen in both normal phase (NP) and RP systems. Therefore, to obtain the best possible LOD and LOQ values of ibuprofen with precoated layers, suitable mobile phase and chromatographic plates are required.

show abstract

Section: Introductionmentioning

confidence: 99%

Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

2020

View full text Add to dashboard Cite

show abstract

“…Several spectrophotometric , chromatographic , and titrimetric methods have been reported for the individual determination of ibuprofen and paracetamol. HPLC method for ibuprofen tablets and column chromatography followed by spectrophotometry for paracetamol tablets are recommended by the United States Pharmacopeia .…”

Section: Introductionmentioning

confidence: 99%

A rapid and sensitive reversed phase‐HPLC method for simultaneous determination of ibuprofen and paracetamol in drug samples and their behaviors in simulated gastric conditions

Borahan

Unutkan

Şahin

et al. 2018

J of Separation Science

View full text Add to dashboard Cite

Paracetamol is a widely used drug for fever and pain relief. Ibuprofen is a common nonsteroidal anti‐inflammatory drug. In this study, a sensitive and accurate reversed phase high performance liquid chromatography method was developed for the simultaneous determination of ibuprofen and paracetamol. The chromatographic separation was achieved on a Phenomenex C18 (250 mm, 4.6 mm, 5 μm) column. Fifty milli molar phosphate buffer (pH 7.5) and methanol were used as mobile phase in a gradient elution mode. The retention times of paracetamol and ibuprofen were 5.7 and 10.4 min, respectively. The linearity of the developed method was established in the range of 0.25 – 250 mg/L with a correlation coefficient of 0.9998 for both analytes. The limit of detection/quantification values were found to be 0.06/0.19 and 0.08/0.26 mg/L for ibuprofen and paracetamol, respectively. The method was successfully applied in drug samples in the form of tablets and suspensions. The calculated concentrations matched with the claimed values on their prospectuses. The drug samples were studied under simulated gastric conditions to determine the behaviors of the analytes in the human body. The obtained results showed no change in the retention time of the analyte peak shapes throughout the 210 minutes.

show abstract

“…In some cases gastrointestinal hemorrhage and dyspepsia are reported. Hypersensitivity reactions occur occasionally, including skin rash, which can be controlled upon immediate withdrawal of therapy (British Pharmacopoeia, 2003;Merck, 2001;Martindale and Westcott, 1996) A number of methods have been reported by various authors for the determination of mefloquine (Lamant et al, 1988;Bergqvist et al, 1988Bergqvist et al, , 1991Bergqvist et al, , 1993Bergqvist et al, , 1994Karbwang et al, 1989;Qiu et al, 1992;Souri et al, 1997;Green et al, 1999), pyrimethamine (Timm and Weidekamm, 1982;Bergqvist et al, 1991;Eljaschewitsch et al, 1996;Bangchang et al, 1997;Astier et al, 1997;Liboux et al, 1991;Zytkovicz et al, 1991;Tracqui et al, 1993;Roberts et al, 1995;Gong et al, 2002;Lin and Jeng, 2002), sulfadoxine (Bhoir et al, 2001;Bonini et al, 1981), and ibuprofen (Sochor et al, 1994(Sochor et al, , 1995Lemko et al, 1993;Castillo and Smith 1993;Rifai et al, 1996;Save et al, 1997;Kepp et al, 1997;Bhushan and Martens, 1998;Kang et al, 1998;Ducret et al, 1998;Gasco-Lopez et al, 1999;Canaparo et al, 2000;Vermeulen and Remon, 2000;…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of pyrimethamine, sulfadoxine, mefloquine, and ibuprofen in pharmaceutical formulations by RP-HPLC

et al. 2009

View full text Add to dashboard Cite

A simple and rapid high-performance liquid chromatographic (HPLC) method for simultaneous determination and quantification of pyramethamine, sulfadoxine, mefloquine HCl and ibuprofen was developed. The chromatographic system consisted of a Shimadzu LC-10 AT VP pump, SPD-10 AV VP UV visible detector, and CBM-102 Bus Module integrator. Separation was achieved on a lBondapak 125 A, C-18, 10-lm column at room temperature. The sample was introduced through an injector valve with a 10-lL sample loop. Acetonitrile/water (50:50, v/v) was used as the mobile phase, with a flow rate of 2 mL/min. The pH was adjusted to 2.6 with phosphoric acid. UV detection was performed at 220 nm. The results obtained showed good agreement with the declared content. Recovery values were from 99.43 to 101.52% for mefloquine (250 mg in Fansimef Ò tablet), from 99.32 to 100.7% for pyrimethamine (25 mg in Fansimef Ò tablet), from 99.29 to 100.21% for sulfadoxine (500 mg in Fansimef Ò tablet), and from 99.96 to 100.04% for ibuprofen (400 mg Dolofen Ò tablet). The proposed method is rapid, accurate, and selective; it may be used for quantitative analysis of pyramethamine, sulfadoxine, mefloquine HCl, and ibuprofen from raw materials, in bulk drugs, and from dosage formulations.

show abstract

High-performance thin-layer chromatographic determination of ibuprofen in plasma

Cited by 21 publications

References 19 publications

Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

A rapid and sensitive reversed phase‐HPLC method for simultaneous determination of ibuprofen and paracetamol in drug samples and their behaviors in simulated gastric conditions

Simultaneous determination of pyrimethamine, sulfadoxine, mefloquine, and ibuprofen in pharmaceutical formulations by RP-HPLC

Contact Info

Product

Resources

About