High-performance thin-layer chromatographic method for the fluorescence detection of three nitroimidazole residues in pork and poultry tissue

Simultaneous determination of seven nitroimidazole residues in meat by using HPLC-UV detection with solid-phase extraction

“…The LOD value for all nitroimidazoles and metabolites was 0.2 g/kg, and the LOQ value was 0.7 g/kg. The obtained detection limit for this method is very lower than that for the methods reported in literature [19][20][21][22][23][24][25]27,28,30]. The proposed method can permit the detection of the seven nitroimidazoles in chiken and porcine tissue samples at level of ≥0.7 g/kg.…”

“…Wang developed a method for determination of the three nitroimidazole residues in poultry meat by gas chromatography with the detection limits of 0.2 g/kg for DMZ and MNZ and 0.5 g/kg for RNZ [16]. Gaugain and Abjean reported a TLC method for the detection of RNZ, DMZ and DMOHZ in animal muscle with the detection limits of 2-5 g/kg [20]. Several HPLC methods were described for the detection and identification of DMZ residues in muscle tissue with a confirmation limit of 5 g/kg [21], as well as DMZ, MNZ in poultry tissue, serum and eggs [22].…”

“…Several analytical methods have been reported for the determination of nitroimidazole either in pure, dosage or in biological fluids and animal tissues, including spectrophotometry [11,12], electrophoresis [13], gas chromatography (GC) [14][15][16], GC-mass spectrometry (MS) [17,18], thin-layer chromatographic (TLC) [19,20], HPLC [21][22][23][24][25][26], and liquid chromatography (LC)-MS [10,[27][28][29][30]. Among them, chromatography has been utilized extensively for the determination of nitroimidazoles.…”

The production and characterisation of dinitrocarbanilide antibodies raised using antigen mimics

“…Methods developed to detect one or more nitroimidazoles have employed gas chromatography-mass spectrometry (GC-MS) [4], liquid chromatography (LC)-MS [5][6][7][8] and high performance thin layer chromatography [9]. In order to screen a large number of samples by immunoassay for the presence of this family of drugs, it was necessary to produce an antibody capable of broad-spectrum compound recognition.…”

Liquid chromatographic tandem mass spectrometric determination of five coccidiostats in poultry eggs and feed

“…Quite a few papers have been published describing the determination of dimetridazole and its main metabolite 2-hydroxydimetridazole in feed, tissues and eggs. In these papers, methods based on techniques such as differential pulse polarography [13], LC [14][15][16][17][18][19][20][21][22], GC [23][24][25][26][27][28][29] and LC-MS [4,5,[30][31][32][33][34][35][36] were described. Out of all the papers which have been published on the determination of halofuginone in different matrices, only few of them used LC-MS [4,5,[37][38][39].…”