High Performance Thin Layer Chromatography (HPTLC) profile of an insecticidal compound of Trochus radiatus and assessment of mucus-associated bacterial extracts

Abstract

The current study is based on the screening of novel insecticides from new sources that have not being exploited hitherto. The major objectives of this research work were to extract marine molluscs, Lambis lambis, Trochus radiatus and Chicoreus ramosus, from Tuticorin coastal waters using different solvents to test their insecticidal properties and partially purify the active components. The ethyl acetate extracts of L. lambis and T. radiatus showed 100% mortality of Sitophilus oryzae, at a concentration of 200 μg ml −1 . The LC 50 values for ethyl acetate extracts of L. lambis, T. radiatus and C. ramosus were found to be 67.08, 348.86 and 571.42 μg ml −1 , respectively. With regard to bacterial metabolites, at a concentration of 200 μg ml −1 the ethyl acetate extract of A3 was able to elicit an activity of 40% and that of strain A1 -20%. The LC 50 values of the bacterial metabolites were also investigated. Upon chromatographic separation of active ethyl acetate extracts of T. radiatus, the 100% methanol column-purified fraction was found to have an activity of 30% at a concentration of 10 μg against S. oryzae. The purity of the partially pure active compound was observed to be 72.78% on analysis with high pressure thin layer chromatography.

Isolation of a Novel Antibacterial Phenyl Thioketone from the Seagrass, Cymodocea serrulata

“…; Wahl, ). Marine floral groups with bioactive chemicals performing significant roles in survival and growth are now being seen as novel and potent drugs and agrochemicals (Gnanambal and Jamila, ; Gnanambal et al ., ; Chellaram et al ., ; Grode et al ., ; Gnanambal et al ., ).…”

“…The study emphasizes that the fractionated leaf extracts of all seagrasses without exception presented better action than their analogous crude extracts could. The enhanced biological activity of the partially purified fractions against their respective crude extracts is described in earlier reports too (Gnanambal et al ., ; Solomon and Santhi, ; Asthana et al ., ).…”

“…A long history is of course, extant, for seagrasses producing assorted secondary metabolites to limit the invasion of microorganisms, epiphytes and predation (McMillan et al, 1980;Buchsbaum et al 1990;Wahl, 2001). Marine floral groups with bioactive chemicals performing significant roles in survival and growth are now being seen as novel and potent drugs and agrochemicals (Gnanambal and Jamila, 2006;Gnanambal et al, 2011;Chellaram et al, 2005;Grode et al, 1983;Gnanambal et al, 2013).…”

Investigation on neurotoxin of sea snail meat

“…Various investigations have proved that T. radiatus and Thais sp. contain a variety of bioactive compounds including a few toxins, which are known to possess potent haemolytic properties [30]. The purified extracts showed maximum haemolytic activity on human erythrocytes followed by poultry.…”

Marine Natural Products: The Important Resource of Biological Insecticide

“…In order to screen novel insecticides from new sources, extracts of three marine molluscs, Lambis , Trochus radiatus and Chicoreus ramosus , were subjected to test their insecticidal properties. The ethyl acetate extracts of L. lambis and T. radiatus showed 100 % mortality of Sitophilus oryzae , at a concentration of 200 mg/ml [95] . Exposure under the methanol and aqueous extracts of marine sponge Iotrochota birotulata can lead the larval affectation to Spodoptera frugiperda , the antifeedant activity of extracts against larvae was also observed [96]…”