High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery

Kulkarni, Rahul; Pandhare, Ramdas; Deshmukh, Vinayak; Mohite, Popat; Pawar, Ashish Y.

doi:10.18231/j.jpbs.2021.002

Cited by 9 publications

(2 citation statements)

References 15 publications

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“…Chromatography on a thin layer (TLC): TLC is a simple and reliable technique for qualitative analysis. Its presence in the sample can be determined by comparing the migration distance of the sample with that of a standard Lagerstroemin [19].…”

Section: Determination Of Lagerstroeminmentioning

confidence: 99%

Antioxidant and hypercholesterolemia effects of Lagerstroemia speciosa ethanolic green and red leaf extracts against Lauric acid and ketogenic diets in female albino rats

Ganesan. G,

Sujatha. P.S.

2023

World J. Adv. Res. Rev.

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The study investigates the therapeutic value of Lagerstroemia speciosa ethanolic green and red leaf extracts against Lauric acid (LA) and a Ketogenic diet (KGD) in Albino rats. Lauric acid is an important constituent in breast milk, forming nutritional factors and having various medicinal values. It is present in 6.2% of human breast milk, 3.1% of goat milk, and 2.9% in cow milk. It is also found in plant sources like coconut, cohune, and palm kernel oil. The Ketogenic diet uses fat, protein, and low carbohydrate for energy. The experimental groups were fed a Ketogenic diet for 21 days, with the treated groups showing a significant increase in blood cell count and Hg levels. The phytochemical profile reveals abundant phytoconstituents, including corosolic acid, lagerstroemin, and anthocyanin, at high levels. The study compared experimental groups fed with LELE Low Dose and High Dose (250 and 500 mg/kg body weight) and standard groups treated with KGD and Lauric acid. The treated groups showed significant elevations in biochemical parameters and increased blood cells and Hg levels. LELE's anti-hypercholesterolemia activity in KGD significantly reduced body weight, total lipid profile, and blood glucose in a dose-dependent manner. The study found that LELE is safe and non-toxic, with the treated group demonstrating dose dependence and decreased obesity.

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Section: Determination Of Lagerstroeminmentioning

confidence: 99%

Antioxidant and hypercholesterolemia effects of Lagerstroemia speciosa ethanolic green and red leaf extracts against Lauric acid and ketogenic diets in female albino rats

Ganesan. G,

Sujatha. P.S.

2023

World J. Adv. Res. Rev.

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“…This complicated instrumental method fully exploits the advantages of thin-layer chromatography. 8 This work aims to develop a responsive, specific, repeatable, and stability-indicating technique for detecting FEB in its presence of its degradation products using a QBD strategy, per ICH guidelines.…”

mentioning

confidence: 99%

Development of Validated Stability Indicating HPTLC Method for Estimation of Febuxostat in Bulk and Tablet Dosage Form by Using QBD Approach

Kharate,

Kuchekar,

Harde

et al. 2022

IJDDT

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Objective: Development of a simple, reliable, time-tested, stability-focused HPTLC method for determining febuxostat (FEB) in bulk and pharmaceutical formulation.Methods: Chloroform, methanol, and formic acid (6.7:2.9:0.1v/v/v) served as the mobile phase, and precoated aluminum TLC plates served as the stationary phase. Active drug FEB was subjected to a densitometric measurement (absorbance mode at 312 nm). The present study performed a comprehensive stress test of FEBas per ICH Q1A(R2) guidelines. FEB has undergone forced degradation by acid, alkali (0.5N HCl and NaOH), oxidative (3% H2O2), H2O and photolytic effect. Results: Calibration plots using HPTLC linear regression analysis indicated a strong correlation (r2 = 0.999) between concentrations from 150 to 900 ng/spot. Accuracy, recoverability, robustness, and documentation are some of the aspects of the method that have been verified. The LoD was 189 ng and the LoQ was 395 ng per location. FEB was not degraded under acid, UV, water, peroxide and photolytic conditions but showed degradation under alkaline conditions. Conclusion: Stability studies and quantification of FEB in bulk and pharmaceutical dose forms by QBD methodology have both been shown to benefit from the suggested HPTLC method. This technique can be utilized as a stability indicator because it successfully isolates the medication from the degradation product.

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Advances of hyphenated technique in impurity profiling of active pharmaceutical ingredients and pharmaceutical products

Khandale,

Rajge,

Singh

et al. 2023

Separation Science Plus

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Impurities found in active pharmaceutical ingredients (APIs) and pharmaceutical products are of ever‐increasing interest. According to several regulatory agencies, purity and impurity profiles are essential. An impurity is defined as any additional inorganic or organic material, residual solvents other than the medicinal components, or undesired compounds that remain with APIs. Impurities and degradation products in bulk drug materials and pharmaceutical formulations are identified, their structures are clarified, and their quantitative determination is part of impurity profiling. Unrecognized, poisonous impurities are dangerous to health and should be identified by selective procedures to increase the safety of drug therapy, and impurity profiling has become more significant in pharmaceutical analysis. This review briefly introduces process and product‐related impurities and emphasizes the creation of cutting‐edge analytical techniques for identifying them. It discusses the use of analytical methods, particularly high‐performance thin‐layer chromatography, liquid chromatography with mass spectrometry (MS), ultrahigh‐performance liquid chromatography, gas chromatography–MS, and nuclear magnetic resonance spectroscopy for the identification of contaminants and degradation products. It has discussed the importance of the quality, efficacy, and safety of drug substances and products, including the origin, types, and quality control of impurities, the need for the development of impurity profiling methods, impurity identification, and regulatory aspects.

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High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery

Cited by 9 publications

References 15 publications

Antioxidant and hypercholesterolemia effects of Lagerstroemia speciosa ethanolic green and red leaf extracts against Lauric acid and ketogenic diets in female albino rats

Antioxidant and hypercholesterolemia effects of Lagerstroemia speciosa ethanolic green and red leaf extracts against Lauric acid and ketogenic diets in female albino rats

Development of Validated Stability Indicating HPTLC Method for Estimation of Febuxostat in Bulk and Tablet Dosage Form by Using QBD Approach

Advances of hyphenated technique in impurity profiling of active pharmaceutical ingredients and pharmaceutical products

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