High-performance thin-layer chromatography – antibacterial assay first reveals bioactive clerodane diterpenes in giant goldenrod (Solidago gigantea Ait.)

Baglyas, Márton; Ott, Péter G.; Garádi, Zsófia; Glavnik, Vesna; Béni, Szabolcs; Vovk, Irena; Móricz, Ágnes M.

doi:10.1016/j.chroma.2022.463308

Cited by 10 publications

(9 citation statements)

References 31 publications

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“…A number of specialised metabolites, including caffeoylquinic acid derivatives, flavonoids, monoterpenes and sesquiterpenes, polyacetylenes, clerodane-type diterpenes, saponins and tannins have been reported in the raw materials of Solidago spp. [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. The corresponding specialised metabolites have been considered as sources of functional ingredients for agriculture, food, cosmetics and pharmaceuticals, in which applications phenolic compounds and essential oils are of great importance.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Root Phenolic Profiles and Antioxidant Activity of Five Native and Invasive Solidago L. Species

Radušienė,

Karpavičienė,

Vilkickytė

et al. 2024

Plants

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The high environmental importance of invasive goldenrod has prompted research to find potential benefits that can be derived from these species. This study aimed to identify differences in root phenolic profiles among five Solidago species, thus providing valuable information on their potential applications and the botanical origin of the raw material. The roots of native S. virgaurea L., two alien species S. gigantea Aiton and S. canadensis L. and their hybrids S. ×niederederi Khek and S. ×snarskisii Gudž. & Žaln. were sampled from mixed-species stands in Lithuania. A complex of twelve phenolic acids and their derivatives was identified and quantified in methanol–water root extracts using the HPLC-PDA and LC/MS systems. The radical-scavenging capacities of the extracts were assessed by ABTS. The chemical content of the roots of S. virgaurea, S. gigantea and S. ×niederederi were statistically similar, while the roots of S. canadensis and S. ×snarskisii contained lower amounts of compounds than the other species. The PCA score-plot models of the phenolic profiles only partially confirmed the identification of S. ×niederederi and S. ×snarskisii as crosses between native and alien species. The findings from the phenolic profiles and the observed radical-scavenging activity of root extracts of Solidago species provide valuable insights into their potential applications in various fields.

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Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Root Phenolic Profiles and Antioxidant Activity of Five Native and Invasive Solidago L. Species

Radušienė,

Karpavičienė,

Vilkickytė

et al. 2024

Plants

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“…The plant is about 0.5-2.5 m tall and has persistent rhizomes [4][5][6]. Giant goldenrod produces a variety of substances with biological effects, including terpenoids, phenolic components, coumarins, essential oils and saponins [7][8][9][10][11][12][13]. Its hydrodistilled volatile oil contains more than 90 components [14].…”

Section: Introductionmentioning

confidence: 99%

“…Its hydrodistilled volatile oil contains more than 90 components [14]. The root and leaf contain large amounts of clerodane-type furanoid diterpenes such as solidagoic acids [12,13]. The antibacterial, antifungal and insecticidal effects of various S. gigantea extracts have also been demonstrated [10,[15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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Disparate Effects of Two Clerodane Diterpenes of Giant Goldenrod (Solidago gigantea Ait.) on Bacillus spizizenii

Bozsó,

Lapat,

Ott

et al. 2024

IJMS

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New substances with antimicrobial properties are needed to successfully treat emerging human, animal, or plant pathogens. Seven clerodane diterpenes, previously isolated from giant goldenrod (Solidago gigantea) root, were tested against Gram-positive Bacillus subtilis, Bacillus spizizenii and Rhodococcus fascians by measuring minimal bactericidal concentration (MBC), minimal inhibitory concentration (MIC) and half-maximal inhibitory concentration (IC50). Two of them, Sg3a (a dialdehyde) and Sg6 (solidagoic acid B), were proved to be the most effective and were selected for further study. Bacillus spizizenii was incubated with the two diterpenes for shorter (1 h) or longer (5 h) periods and then subjected to genome-wide transcriptional analyses. Only a limited number of common genes (28 genes) were differentially regulated after each treatment, and these were mainly related to the restoration of cell membrane integrity and to membrane-related transports. Changes in gene activity indicated that, among other things, K+ and Na+ homeostasis, pH and membrane electron transport processes may have been affected. Activated export systems can be involved in the removal of harmful molecules from the bacterial cells. Inhibition of bacterial chemotaxis and flagellar assembly, as well as activation of genes for the biosynthesis of secondary metabolites, were observed as a general response. Depending on the diterpenes and the duration of the treatments, down-regulation of the protein synthesis-related, oxidative phosphorylation, signal transduction and transcription factor genes was found. In other cases, up-regulation of the genes of oxidation–reduction processes, sporulation and cell wall modification could be detected. Comparison of the effect of diterpenes with the changes induced by different environmental and nutritional conditions revealed several overlapping processes with stress responses. For example, the Sg6 treatment seems to have caused a starvation-like condition. In summary, there were both common and diterpene-specific changes in the transcriptome, and these changes were also dependent on the length of treatments. The results also indicated that Sg6 exerted its effect more slowly than Sg3a, but ultimately its effect was greater.

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“…Furthermore, TLC-based bioassays played an important role in the isolation of insect kairomones from complex mixtures such as EOs. Although it is not a new technique, TLC continues to be a valuable tool as a preparative technique for a variety of studies in both chemical and biological fields [25,[32][33][34][35]. While gas chromatography-mass spectrometry (GC-MS) is exceptional in the identification of unknown chemicals, it works best for trace analysis [36].…”

Section: Introductionmentioning

confidence: 99%

HPTLC Analysis and Chemical Composition of Selected Melaleuca Essential Oils

2023

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Tea tree oil (TTO) is a volatile essential oil obtained by distillation, mainly from the Australian native plant Melaleuca alternifolia (Maiden & Betche) Cheel (Myrtaceae). In this study, a comparative analysis of the chemical constituents of seven tea tree oils (M. alternifolia) and four other Melaleuca spp. oils (M. cajuputi, (MCa), two chemotypes of M. quinquenervia, (MNe and MNi), and M. ericifolia (MRo)) was carried out using gas chromatography–mass spectrometry (GC-MS) and high-performance thin-layer chromatography (HPTLC). Among the seven TTOs, terpinen-4-ol (37.66–44.28%), γ-terpinene (16.42–20.75%), α-terpinene (3.47–12.62%), α-terpineol (3.11–4.66%), and terpinolene (2.75–4.19%) were the most abundant compounds. On the other hand, the most abundant compounds of the other Melaleuca oils varied, such as 1,8-cineole (64.63%) in MCa oil, (E)-nerolidol (48.40%) and linalool (33.30%) in MNe oil, 1,8-cineole (52.20%) in MNi oil, and linalool (38.19%) and 1,8-cineole (27.57%) in MRo oil. HPTLC fingerprinting of Melaleuca oils enabled the discrimination of TTO oils from other Melaleuca spp. oils. Variation was observed in the profile of the Rf values among EOs. The present study shows that HPTLC is one of the best ways to identify and evaluate the quality control in authenticating TTOs, other Melaleuca EOs, or EOs from other species within the Myrtaceae.

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High-performance thin-layer chromatography – antibacterial assay first reveals bioactive clerodane diterpenes in giant goldenrod (Solidago gigantea Ait.)

Cited by 10 publications

References 31 publications

Comparative Analysis of Root Phenolic Profiles and Antioxidant Activity of Five Native and Invasive Solidago L. Species

Comparative Analysis of Root Phenolic Profiles and Antioxidant Activity of Five Native and Invasive Solidago L. Species

Disparate Effects of Two Clerodane Diterpenes of Giant Goldenrod (Solidago gigantea Ait.) on Bacillus spizizenii

HPTLC Analysis and Chemical Composition of Selected Melaleuca Essential Oils

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