High-Performance Thin-Layer Chromatography (HPTLC) Method for Identification of Meloxicam and Piroxicam

Abstract

Background: High-performance thin-layer chromatography (HPTLC) is an advantageous, modern analytical technique based on the principles of thin-layer chromatography (TLC), which provides essential benefits, such as improved sample application, better and faster separation, and less mobile phase usage. The aim of this work was to develop a simple and rapid HPTLC method that could be used for the identification of meloxicam and piroxicam. Methods: HPTLC. An analysis was carried out using silica gel 60 F254 glass TLC plates and as the mobile phase: hexane: ethyl acetate: glacial acetic acid, in a ratio of 65:30:5 v/v/v. For the standard solution preparation, ethanol was used. Front: 60 mm. Detection was performed at 366 nm. Results: The Rf value for meloxicam was 0.81 and the Rf value for piroxicam was 0.57. The proposed method can be used in the detection of the analyzed compounds in very low concentrations. It was established that the detection limit of meloxicam was 0.04 μg per band and that of piroxicam was 0.05 μg per band. It was also established that the quantitation limit of meloxicam was 0.12 μg per band and that of piroxicam was 0.15 μg per band. Conclusions: The proposed method is simple, sensitive, stable, cost effective, and eco-friendly. It could be used in research or for routine quality control purposes.

Anti-Adipogenic Activity of Rhaponticum carthamoides and Its Secondary Metabolites

“…The HPTLC technique has several advantages, including rapidity, cost-effectiveness, etc. [51,52], making it a highly suitable method for the future analysis of PDs.…”

“…The HPTLC technique has several advantages, including rapidity, cost-effectiveness, etc. [51,52], making it a highly suitable method for the future analysis of PDs. - [50] Except for the traditional adaptogenic activity of RCE, diverse biological activities are reported either for the extract or for the PDs investigated in this study.…”

Development of a thin layer chromatographic method for the determination of coenzyme Q10produced byAgrobacterium tumefaciens

“…The TLC‐based methods are commonly used for routine analysis of various compounds due to their simplicity, cost‐effectiveness, and speed. TLC brings extra benefits in terms of sample and solvent preparation (no prior filtration or degassing), reusability of solvents, and analysis of many samples simultaneously [21, 22]. To the best of our knowledge, there is no such TLC‐based method reported for the detection of CoQ 10 produced and extracted from A. tumefaciens .…”

Optimization, Identification, and Quantification of Selected Phenolics in Three Underutilized Exotic Edible Fruits Using HPTLC

“…High-performance thin-layer chromatography (HPTLC) is a modern, powerful analytical technique that can provide the advantages of a small amount of sample application; simplicity; better, faster, and more efficient separation; a very small amount of mobile phase used; and ease in handling various applications [31]. Nowadays, HPTLC is a widely used analytical technique in the analysis of food constituents, pharmaceuticals, clinical, drug formulations, botanicals, environmental samples, impurities, and quality control [32,33]. HPTLC can be used for the simultaneous identification and quantification of bioactive components of plants.…”