High-performance thin-layer chromatography (HPTLC) method development and validation for the quantification of catechin in the hydroalcoholic extract of Parkia roxburghii seed

High‐performance thin layer chromatography method development, validation, and assessment of enzymatic inhibition potential of Potentilla fulgens hydroalcoholic roots extract

“…assamica extracts and its validation was performed using mobile phase toluene: ethyl acetate: acetone: formic acid (6:6:6:1, v/v/v/v) [29]. In another study, the HPTLC method was used to determine catechin in the hydroalcoholic extract of Parkia roxburghii seed by a densitometric method using the mobile phase ethyl acetate, acetic acid, formic acid, water (10:1:0.75:1, v/v/v/v) [4]. This was the first detailed HPTLC analysis of catechin in the Potentilla fulgens hydroalcoholic roots extract.…”

“…High-performance thin-layer chromatography has become an effective tool for the quality control of herbal medicines due to its ease and consistency. It can be implemented for the identification, verification, and quality control of herbal medicines [4]. Extracts/compounds comprising the free radical scavenging activity show great perspective in improving many diseases [5].…”

Reversed phase‐high‐performance liquid chromatography simultaneous estimation of phenolic compounds in hydroalcoholic extract of Potentilla fulgens root

“…The most common catechin isomer is (+)-catechin and the other stereoisomer is (-)-catechin (Figure 1) [3]. Previous biological studies on Potentilla fulgens have shown that its root extract holds potent antioxidant activity [4][5][6] and improves age-related oxidative stress and associated diseases [7], anticancer activity [8,9], antimicrobial activity [9], useful in eye disorders convinced by traumatic brain injury [10], gastroprotective activity [1], anti-diabetic activity [11][12][13], neuroprotective activity [14], anti-acetylcholinesterase and anti-hydroxymethylglutaryl-coenzyme A reductase activity [6] and anthelmintic activity [15].…”