High-Performance Thin Layer Chromatography Method Development to Estimate Phytoconstituents in Hedychium species

Abstract

Objective: The H. coronarium (Zingiberaceae) is a perennial herbaceous plant. Chinese immigrants introduced the H. coronarium species as an ornamental plant in 1888. Its attractive fl owers make it a desirable ornamental plant. The present study aims to evaluate the physiochemical characteristics and development of the (HPTLC) Fingerprint sequence profi le of Hedychium coronarium leaves. Method: Pharmacognostic characteristics and physiochemical parameters were performed according to the method prescribed. The mobile phase confi rmation was completed using by performing (TLC). The development of the HPTLC fi ngerprint was performed with some modifi cations to get exact separation of phytochemicals using mobile phases such as Toluene: chloroform: ethanol (4:4:1). Result: Pharmacognostic study reported the presence of unicellular trichomes, vessels, epidermal cells, oil globules, and vascular bundles. Pharmacognostic and physiochemical parameters like color, nature, odor, taste, foreign organic matter, loss on drying, ash value, extractive value, and the foaming index were found to be satisfi ed as documented in the results. The HPTLC fi ngerprinting reveals the presence of various chemical compounds expressed as Rf value. Conclusion: From current scientifi c research work, Hedychium coronarium can be useful in modern medicine as various bioactive secondary metabolites were detected.