High-power lactate/O2 enzymatic biofuel cell based on carbon cloth electrodes modified with MgO-templated carbon

Shitanda, Isao; Takamatsu, Koutaro; Niiyama, Ayumu; Mikawa, Tsutomu; Hoshi, Yoshinao; Itagaki, Masayuki; Tsujimura, Seiya

doi:10.1016/j.jpowsour.2019.226844

Cited by 73 publications

(52 citation statements)

References 53 publications

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“…Some studies have proposed ways for the use of MCOs in cathodes for the ORR in biofuel cells, obtaining current densities up to several mA cm −2 (ref. 10 ). Bilirubin oxidases (BOD) belong to the group of MCOs that have 4 copper ions in its reactive site, divided into three groups, namely T1, T2, and T3 ( Fig.…”

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confidence: 99%

Assessing electron transfer reactions and catalysis in multicopper oxidases with operando X-ray absorption spectroscopy

et al. 2020

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Here we propose an experimental setup based on operando X-ray absorption spectroscopy (XAS) to understand why copper-containing oxidoreductase enzymes show exceptional performance as catalysts for the oxygen reduction reaction (ORR). An electrode based on carbon nanoparticles organized in mesoporous structures with bilirubin oxidase (BOD) was developed to be used in a home-made operando XAS electrochemical cell, and we probed the electron transfer under ORR regime. In the presence of molecular oxygen, the BOD cofactor containing 4 copper ions require an overpotential about 150 mV to be reduced as compared to that in the absence of oxygen. A second electron transfer step, which occurs faster than the cofactor reduction, suggests that the cooper ions act as a tridimensional redox active electronic bridges for the electron transfer reaction.

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confidence: 99%

Assessing electron transfer reactions and catalysis in multicopper oxidases with operando X-ray absorption spectroscopy

et al. 2020

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“…By contrast, the formation of liquid droplets is a simple method for improving enzyme activity because only the addition of a polyelectrolyte into the enzyme solution is needed. LOX is an oxidoreductase applied to biofuel cells 18 and biosensors. 30 Thus, the formation of liquid droplets represents a versatile approach for improving enzyme activity for practical applications using LOX or other enzymes.…”

Section: Discussionmentioning

confidence: 99%

Hyperactivation of L-lactate oxidase by liquid-liquid phase separation

Ura

Kagawa

Yagi

et al. 2020

Preprint

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Liquid droplets formed by liquid-liquid phase separation are attracting attention as functional states of proteins in living cells. Liquid droplets are thought to activate enzymatic reactions by assembling the required molecules. Thus, liquid droplets usually increase the affinity of an enzyme to its substrates, leading to decreased KM values. In this study, we demonstrate a new mechanism of enzyme activation in the droplets using Llactate oxidase (LOX). In the presence of poly-L-lysine (PLL), LOX formed droplets with diameters of hundreds of nanometers to tens of micrometers, stabilized by electro-static interaction. The enzyme activity of LOX in the droplets was significantly enhanced by a fourfold decrease in KM and a tenfold increase in kcat. To our knowledge, this represents the first report for increasing kcat by the formation of the liquid droplet. Interestingly, the conformation of LOX changed in the liquid droplet, probably leading to increased kcat value. Understanding enzyme activation in the droplets provides essential information about enzyme function in living cells in addition to biotechnology applications.

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“…These phenomena can also seriously impede the redox reaction kinetics, resulting in a low energy conversion efficiency. [ 257 ] Furthermore, given that the energy efficiency of all the BFCs reported to date is not high enough to serve as a practical power source, more considerable research efforts are required to improve the charge transfer efficiency and the resultant power output of BFCs by designing effective interfacial interactions and structures as well as high‐performance electrode materials. To expand the applications of and commercialize BFCs, the BFC output performance not only in area power density but also in net power must be increased.…”

Section: Flexible/wearable Energy Applicationsmentioning

confidence: 99%

Interfacial Design and Assembly for Flexible Energy Electrodes with Highly Efficient Energy Harvesting, Conversion, and Storage

Lee

Kwon

et al. 2021

Advanced Energy Materials

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Charge transfer between a conductive support and active materials as well as between neighboring active materials is one of the most critical factors in determining the performance of various electrodes in energy harvesting, conversion, and/or storage. Particularly, when preparing energy electrodes using conductive and/or electrochemically active nanoparticles (NPs), the bulky organic materials (i.e., ligand or polymeric binder) covering the NP surface seriously limit the charge transfer within the electrode, thereby restricting the energy storage or conversion efficiency. Furthermore, the flexibility and mechanical stability of the electrode have been considered important evaluation indices for flexible/wearable energy applications. In this regard, considerable research has been directed toward controlling the interfacial structure to enhance the charge transfer efficiency and toward incorporating functional materials into flexible/porous supports. This review describes the central progress in flexible electrodes for energy harvesting, conversion, and storage, along with the challenges in designing highperformance energy electrodes. In particular, layer-by-layer (LbL) assembly is analyzed, which is an ultrathin film fabrication technology that enables fine tuning of the interfacial structure for various electrode materials. It is shown how LbL assembly can be effectively applied to energy electrodes to obtain desired functionalities and improve the charge transfer efficiency of electrodes.

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High-power lactate/O2 enzymatic biofuel cell based on carbon cloth electrodes modified with MgO-templated carbon

Cited by 73 publications

References 53 publications

Assessing electron transfer reactions and catalysis in multicopper oxidases with operando X-ray absorption spectroscopy

Assessing electron transfer reactions and catalysis in multicopper oxidases with operando X-ray absorption spectroscopy

Hyperactivation of L-lactate oxidase by liquid-liquid phase separation

Interfacial Design and Assembly for Flexible Energy Electrodes with Highly Efficient Energy Harvesting, Conversion, and Storage

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