High-precision isotopic analysis of serum and whole blood Cu, Fe and Zn to assess possible homeostasis alterations due to bariatric surgery

Hastuti, Agustina A. M. B.; Costas‐Rodríguez, Marta; Anoshkina, Yulia; Parnall, Taylor; Madura, James A.; Vanhaecke, Frank

doi:10.1007/s00216-019-02291-2

Cited by 22 publications

(33 citation statements)

References 53 publications

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“…Our data align those previous results and further elaborate on serum δ 65 Cu values within different NAFLD stages. In agreement with previously published data of a bariatric cohort [11], serum δ 65 Cu values were lower in NAFLD patients. The variation of serum δ 65 Cu values in different stages of NAFLD had not been investigated thus far, and we show that, already at the level of simple steatosis, serum δ 65 Cu values are lower, and remain stable during further disease progression.…”

Section: Discussionsupporting

confidence: 92%

“…A larger patient cohort could have also allowed to evaluate assay reproducibility and sensitivity. Nevertheless, serum δ 65 Cu values have previously been shown to be reproducible in healthy controls [11][12][13], so that these results seem highly reliable.…”

Section: Limitationsmentioning

confidence: 93%

“…63 Cu and 65 Cu are the two stable Cu isotopes that occur naturally, showing small, but systematic variations in their abundance ratio under certain disease conditions that can be reliably quantified [8][9][10]. It has been published recently that patients destined for bariatric surgery may show lower serum δ 65 Cu values [11]. Bariatric surgery is performed on morbidly obese patients to reduce food intake and obtain weight loss, and NAFLD is a prominent health risk in these patients.…”

Section: Introductionmentioning

confidence: 99%

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Lighter serum copper isotopic composition in patients with early non-alcoholic fatty liver disease

et al. 2020

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Objective: The occurrence of non-alcoholic fatty liver disease (NAFLD) is globally increasing. To challenge the current incidence of NAFLD, non-invasive markers that could identify patients at risk or monitor disease progression are an important need. Copper intake and organ copper concentrations have earlier been linked to NAFLD progression, but serum copper does not adequately represent the disease state. Cu atoms occur under the form of two stable isotopes, 63 Cu and 65 Cu, and the ratio of both (expressed as δ 65 Cu, in ‰) in blood serum has been shown to be altered in chronic liver disease. To assess whether the Cu isotope ratio might predict disease occurrence and progression of NAFLD, the serum Cu isotopic composition of patients with different stages of NAFLD was determined. Results: Our results showed that serum δ 65 Cu values were lower in NAFLD patients, already at the level of simple steatosis, and remained stable during further disease progression. ROC analysis shows an almost perfect diagnostic ability of serum δ 65 Cu values for NAFLD, but no discrimination between different severity degrees could be made. Therefore, the serum Cu isotopic composition might show potential for early diagnosis of NAFLD patients.

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Section: Discussionsupporting

confidence: 92%

Section: Limitationsmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Lighter serum copper isotopic composition in patients with early non-alcoholic fatty liver disease

et al. 2020

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“…Establishing the isotopic variability within a healthy population is crucial, as the reference range might be affected by nutritional and metal status, basal metabolic rate, geographical origin, gender, age, etc. Although the Fe, Cu and Zn isotopic compositions in blood plasma, serum, red blood cells or whole blood of healthy individuals from different geographical origins have been documented, data are still scarce and furthermore, only a few works reported the isotopic composition of the three elements for the same sample/individual 17,18 . The plasma Fe, Cu and Zn isotopic compositions for each individual are provided in Table S1.…”

Section: Resultsmentioning

confidence: 99%

“…The Cu fraction was eluted using 9 mL of 5 M HCl + ~ 0.001% H 2 O 2 , the Fe fraction using 7 mL of 0.6 M HCl and the Zn fraction using 7 mL of 0.7 M HNO 3 . The Cu fractions were subjected to a second column pass to ensure a Na/Cu ratio < 2 in all solutions 17,22 . The pure fractions thus obtained were subjected to two steps of drying and re-dissolution in 14 M HNO 3 to remove residual chlorides and, were finally re-dissolved in 0.5 mL of 0.42 M HNO 3 for high-precision isotope ratio measurements using MC-ICP-MS. For elemental analysis, plasma samples were digested with 0.5 mL of 14 M HNO 3 (Tamapure-AA-100, Tama Chemical Co. Ltd., Kanagawa, Japan) at 180 °C for 20 min using an ETHOS 1 microwave dissolution unit (Milestone, Shelton, CT, USA) and then diluted with Milli-Q water (Nihon Millipore, Tokyo, Japan) to a final volume of 5 mL.…”

Section: Discussionmentioning

confidence: 99%

Cu and Zn isotope ratio variations in plasma for survival prediction in hematological malignancy cases

Hastuti

Costas‐Rodríguez

Matsunaga

et al. 2020

Sci Rep

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We have examined potential changes in the isotopic compositions of Fe, Cu and Zn (using multi-collector inductively coupled plasma-mass spectrometry) and the corresponding concentrations (using inductively coupled plasma-atomic emission spectrometry) in plasma from hematological malignancy (HM) patients and assessed their prognostic capability. Together with clinical laboratory test values, data were examined in view of a 5-years survival prediction. Plasma Cu and Zn isotope ratios and their concentrations were significantly different in HM patients compared to matched controls (P < 0.05). Both δ65Cu and δ66Zn values showed significant mortality hazard ratios (HRs) in HM. The group of patients with decreased δ65Cu and increased δ66Zn values showed significantly poorer survival from the early phase (HR 3.9; P = 0.001), forming a unique cohort not identified based on laboratory test values. Well-known prognostic factors for HM, such as the creatinine level, and anemia-related values were highly correlated with the δ66Zn value (P < 0.05). Time-dependent ROC curves based on the δ65Cu or δ66Zn value were similar to that based on the creatinine concentration (a well-known prognostic factor in HM), indicating that δ65Cu or δ66Zn values are useful for prognosis of HM. Variations in stable isotope ratios of essential mineral elements have thus been shown to reflect alterations in their homeostasis due to physiological changes in malignancies with higher sensitivity than concentrations do.

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Sweat chloride assay by inductively coupled plasma mass spectrometry: a confirmation test for cystic fibrosis diagnosis

et al. 2020

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The current guidelines for sweat chloride analysis identify the procedures for sweat collection, but not for chloride assay, which is usually performed by methods originally not aiming at the low concentrations of chloride found in sweat. To overcome this limitation, we set up, characterized, and adopted an original inductively coupled plasma mass spectrometry (ICP-MS) method for sweat chloride determination, which was designed for its easy use in a clinical laboratory. The method was linear in the range 8.5E−3 to 272.0E−3 mM, precision exhibited a relative standard deviation < 6%, and accuracy was in the range 99.7–103.8%. Limit of blank, limit of detection, and limit of quantitation were 2.1 mM, 3.2 mM, and 7.0 mM, respectively, which correspond to real concentrations injected into the mass spectrometer of 3.9E−3 mM for LOD and 8.5E−3 mM for LOQ. At first, the method was tested on 50 healthy volunteers who exhibited a mean chloride concentration of 15.7 mM (25–75th percentile 10.1–19.3 mM, range 2.8–37.4 mM); then, it was used to investigate two patients with suspected cystic fibrosis, who exhibited sweat chloride values of 65.6 mM and 81.2 mM, respectively. Moreover, the method was cross-validated by assaying 50 samples with chloride concentration values in the range 10–131 mM, by both ICP-MS and coulometric titration, which is the technology officially used in Tuscany for cystic fibrosis newborn screening. The reference analytical performances and the relatively low cost of ICP-MS, accompanied by the advantageous cost of a single sweat chloride assay, make this technology the best candidate to provide a top reference method for the quantification of chloride in sweat. The method that we propose was optimized and validated for sweat samples ≥ 75 mg, which is the minimum amount requested by the international protocols. However, the method sensitivity and, in addition, the possibility to reduce the sample dilution factor, make possible the quantification of chloride even in samples weighting < 75 mg that are discarded according to the current guidelines.

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High-precision isotopic analysis of serum and whole blood Cu, Fe and Zn to assess possible homeostasis alterations due to bariatric surgery

Cited by 22 publications

References 53 publications

Lighter serum copper isotopic composition in patients with early non-alcoholic fatty liver disease

Lighter serum copper isotopic composition in patients with early non-alcoholic fatty liver disease

Cu and Zn isotope ratio variations in plasma for survival prediction in hematological malignancy cases

Sweat chloride assay by inductively coupled plasma mass spectrometry: a confirmation test for cystic fibrosis diagnosis

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