High-Precision Lens-Less Flow Cytometer on a Chip

Fang, Yuan; Yu, Ningmei; Jiang, Yuquan; Dang, Chaoliang

doi:10.3390/mi9050227

Cited by 21 publications

(13 citation statements)

References 31 publications

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“…To keep track of droplets, microscopic tags have been developed to enable later reading of their content and correlate the different measurements performed over time. 108,192,[209][210][211] It has also been shown that by confining the droplets in a line in capillaries or tubing, it is possible to keep track of the content of each droplet without tagging them and individually access them. This has been used for instance to study the proliferation of different cell populations over time.…”

Section: Perspectives On Catalyst Characterization In Microfluidicsmentioning

confidence: 99%

Microfluidics and catalyst particles

et al. 2019

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In this review article, we discuss the latest advances and future perspectives of microfluidics for micro/ nanoscale catalyst particle synthesis and analysis. In the first section, we present an overview of the different methods to synthesize catalysts making use of microfluidics and in the second section, we critically review catalyst particle characterization using microfluidics. The strengths and challenges of these approaches are highlighted with various showcases selected from the recent literature. In the third section, we give our opinion on the future perspectives of the combination of catalytic nanostructures and microfluidics. We anticipate that in the synthesis and analysis of individual catalyst particles, generation of higher throughput and better understanding of transport inside individual porous catalyst particles are some of the most important benefits of microfluidics for catalyst research. SynthesisThis section focuses on the most recent approaches within the last 8 years. For a more extensive overview of the synthesis of nanostructures focused on the processes taking place inside the microfluidic systems, 5 the final application 4 or the microfluidic technology used 6,7 we refer the reader to other review articles. Metal nanoparticlesMetal nanoparticles exhibit very interesting catalytic, optical, chemical, electromagnetic and magnetic properties, all of them depending to a large degree on their size and composition. Regarding catalysis, a decrease of the NP size leads to a surface-area increase per mass, providing more active sites. Also, the surface structure is of vital importance for the NP selectivity: the presence of steps, edges or terraces in the atomic surface can influence the reaction pathways to Lab Chip, 2019, 19, 3575-3601 | 3575 This journal is

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Section: Perspectives On Catalyst Characterization In Microfluidicsmentioning

confidence: 99%

Microfluidics and catalyst particles

et al. 2019

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“…Due to the missing phase, the in-line holography technique has inherent zero-level image interference. IT can be iteratively eliminated using the zero-level image method we studied earlier [33]. Through the above algorithm, a clear high-resolution image of the cells can be reconstructed.…”

Section: Reconstruction Of the Focus Plane Image Algorithmmentioning

confidence: 99%

Super-Resolution Lensless Imaging of Cells Using Brownian Motion

Fang

Jiang

2019

Applied Sciences

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The lensless imaging technique, which integrates a microscope into a complementary metal oxide semiconductor (CMOS) digital image sensor, has become increasingly important for the miniaturization of biological microscope and cell detection equipment. However, limited by the pixel size of the CMOS image sensor (CIS), the resolution of a cell image without optical amplification is low. This is also a key defect with the lensless imaging technique, which has been studied by a many scholars. In this manuscript, we propose a method to improve the resolution of the cell images using the Brownian motion of living cells in liquid. A two-step algorithm of motion estimation for image registration is proposed. Then, the raw holographic images are reconstructed using normalized convolution super-resolution algorithm. The result shows that the effect of the collected cell image under the lensless imaging system is close to the effect of a 10× objective lens.

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“…Finally, Fang et al [11] presented a low cost and small size flow cytometer on a microfluidic chip, integrating an inline lens-free holographic microscope. The authors proposed an S-type microchannel with a pulse injection flow, and obtained a less than 2% cell counting error.…”

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confidence: 99%

“…The authors proposed an S-type microchannel with a pulse injection flow, and obtained a less than 2% cell counting error. This on-chip flow cytometer can continuously count cells and continuously collect a large number of cell images for subsequent cell analysis, and is in full compliance with the current development trend of point-of-care testing [11].…”

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confidence: 99%