High Predictive Power of Meat Juice Serology on the Presence of Hepatitis E Virus in Slaughter Pigs

Dzierzon, Janine; Oswaldi, Verena; Merle, Roswitha; Langkabel, Nina; Meemken, Diana

doi:10.1089/fpd.2020.2797

Cited by 6 publications

(16 citation statements)

References 33 publications

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“…Pigs originated from 25 German conventional fattening farms, of which ten slaughter pigs were randomly selected at the abattoir. The HEV seroprevalence and the prevalence of HEV-RNA in the liver of those pigs was previously determined and published (Dzierzon et al 2020). Therefore, the HEV detection rate of 250 livers was available and considered as the initial HEV status of each liver for the present study.…”

Section: Methodsmentioning

confidence: 99%

“…The sampling procedure along the slaughter line has been described previously (Dzierzon et al 2020). In addition, each carcass and liver was numbered before sampling, in order to assign each sample to the pig of origin.…”

Section: Sample Collection and Processingmentioning

confidence: 99%

“…In Europe, the HEV seroprevalence in pigs amounts up to 96% at single animal basis and up to 97% at herd level, highlighting that HEV circulates in European pig populations (Wutz et al 2013;Burri et al 2014;Caruso et al 2017;Feurer et al 2018;Dzierzon et al 2020). HEV infection in pigs occurs usually without clinical symptoms, so there is no possibility to detect HEV infected pigs macroscopically during routine ante-and post-mortem inspection at the abattoir (Meng et al 1997;van der Poel et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…HEV RNA detection in different organs and matrices of pigs during slaughter as well as in abattoir's effluents and on different surfaces along the slaughter line indicates that HEV circulates in pigs and in the abattoir's environment (Fenaux et al 2018;Feurer et al 2018;Garcia et al 2019;Milojević et al 2019;Dzierzon et al 2020). It is considered that temperature is the most effective option for inactivation of HEV in food and in the environment (EFSA 2017).…”

Section: Introductionmentioning

confidence: 99%

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Hepatitis E virus cross-contamination on the surface of porcine livers after storage in Euro meat containers in a German pig abattoir

Dzierzon

Oswaldi

Merle

et al. 2021

J Consum Prot Food Saf

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Hepatitis E virus (HEV) is a foodborne zoonotic pathogen and known as the causative agent of hepatitis E in humans. The specific role of porcine liver as a vehicle for human HEV infections has been highlighted in different studies. Nevertheless, gaps of knowledge still exist regarding possible HEV cross-contamination both at consumer and production level. Furthermore, people working in the food production industry, e.g. veterinarians and abattoir employees, are exposed to an increased risk of HEV infection. The aim of the present study was to investigate HEV cross-contamination on the surface of porcine liver in a German abattoir. The sample set included 250 samples of porcine liver parenchyma and the corresponding 250 superficial layer samples of the same livers, which were analyzed for the presence of HEV ribonucleic acid (RNA). Afterwards, the initial status of the tested liver parenchyma was compared with the occurrence of HEV RNA in the corresponding superficial layer. HEV RNA was detectable in 34% (85/250) of superficial layer samples, with 58% (49/85) of the samples originated from initially HEV negative livers. To our knowledge, this is the first study that provides an insight in the potential of HEV cross-contamination at abattoir level in Germany. Furthermore, it could be identified that the joint storage of livers in Euro meat containers has a significant impact on the presence of HEV RNA on the surface of porcine liver.

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Section: Methodsmentioning

confidence: 99%

Section: Sample Collection and Processingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hepatitis E virus cross-contamination on the surface of porcine livers after storage in Euro meat containers in a German pig abattoir

Dzierzon

Oswaldi

Merle

et al. 2021

J Consum Prot Food Saf

Self Cite

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“…Although concerns have arisen from the use of meat drip for Salmonella monitoring on several occasions [75,76], an important driver to assay meat drip instead of blood serum is that it is practically easier than collecting blood samples at for example slaughter [77,78]. It is also a matrix with high predictive power for the occurrence of HEV in pork [79]. Its use to screen other pathogens, such as the protozoan Toxoplasma gondii in other food animals, including goats and sheep [80,81], has been well demonstrated.…”

Section: Measuring Principle Of Indirect Antigen Testsmentioning

confidence: 99%

Modernization of Control of Pathogenic Micro-Organisms in the Food-Chain Requires a Durable Role for Immunoaffinity-Based Detection Methodology—A Review

Bergwerff

Debast

2021

Foods

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Food microbiology is deluged by a vastly growing plethora of analytical methods. This review endeavors to color the context into which methodology has to fit and underlines the importance of sampling and sample treatment. The context is that the highest risk of food contamination is through the animal and human fecal route with a majority of foodborne infections originating from sources in mass and domestic kitchens at the end of the food-chain. Containment requires easy-to-use, failsafe, single-use tests giving an overall risk score in situ. Conversely, progressive food-safety systems are relying increasingly on early assessment of batches and groups involving risk-based sampling, monitoring environment and herd/flock health status, and (historic) food-chain information. Accordingly, responsible field laboratories prefer specificity, multi-analyte, and high-throughput procedures. Under certain etiological and epidemiological circumstances, indirect antigen immunoaffinity assays outperform the diagnostic sensitivity and diagnostic specificity of e.g., nucleic acid sequence-based assays. The current bulk of testing involves therefore ante- and post-mortem probing of humoral response to several pathogens. In this review, the inclusion of immunoglobulins against additional invasive micro-organisms indicating the level of hygiene and ergo public health risks in tests is advocated. Immunomagnetic separation, immunochromatography, immunosensor, microsphere array, lab-on-a-chip/disc platforms increasingly in combination with nanotechnologies, are discussed. The heuristic development of portable and ambulant microfluidic devices is intriguing and promising. Tant pis, many new platforms seem unattainable as the industry standard. Comparability of results with those of reference methods hinders the implementation of new technologies. Whatever the scientific and technological excellence and incentives, the decision-maker determines this implementation after weighing mainly costs and business risks.

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Das Hepatitis-E-Virus – ein zoonotisches Virus: Verbreitung, Übertragungswege und Bedeutung für die Lebensmittelsicherheit

Johne

Althof

Nöckler

et al. 2022

Bundesgesundheitsbl

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ZusammenfassungDas Hepatitis-E-Virus (HEV) ist ein Erreger einer akuten Hepatitis beim Menschen. Darüber hinaus treten zunehmend auch chronische Infektionen mit fataler Leberzirrhose bei immunsupprimierten Transplantationspatienten auf. Die Zahl der gemeldeten Hepatitis-E-Fälle in Deutschland hat in den vergangenen Jahren stark zugenommen. Hier kommt vor allem der Genotyp 3 vor, der zoonotisch von Tieren auf den Menschen übertragen werden kann. Haus- und Wildschweine, die ohne die Ausbildung klinischer Symptome infiziert werden, stellen das Hauptreservoir dar. In diesem Artikel werden die Verbreitung von HEV in Tieren in Deutschland, mögliche Übertragungswege des Virus und insbesondere die Bedeutung von Lebensmitteln bei der Übertragung anhand der aktuellen wissenschaftlichen Literatur dargestellt.HEV ist in Haus- und Wildschweinen in Deutschland stark verbreitet und wird hauptsächlich über direkten Kontakt oder den Verzehr von Lebensmitteln, die aus diesen Tieren hergestellt wurden, auf den Menschen übertragen. Beim HEV-RNA-Nachweis in spezifischen Lebensmitteln bleibt allerdings oft unklar, ob das enthaltene Virus noch infektiös ist oder durch die Herstellungsbedingungen inaktiviert wurde. Neuere Studien weisen auf eine hohe Stabilität des HEV unter verschiedenen physikochemischen Bedingungen hin, wohingegen eine Inaktivierung unter anderem durch Erhitzung erreicht wird. Generell wird deshalb ein ausreichendes Erhitzen von Schweinefleisch und -leber vor dem Verzehr empfohlen und für Risikogruppen zusätzlich der Verzicht auf den Verzehr kurzgereifter Rohwürste.Weitere Forschungen sind nötig, um relevante Risikolebensmittel zu identifizieren, alternative Übertragungswege zu untersuchen und effiziente Maßnahmen zu entwickeln, die eine zoonotische Virusübertragung zukünftig verringern oder vermeiden.

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High Predictive Power of Meat Juice Serology on the Presence of Hepatitis E Virus in Slaughter Pigs

Cited by 6 publications

References 33 publications

Hepatitis E virus cross-contamination on the surface of porcine livers after storage in Euro meat containers in a German pig abattoir

Hepatitis E virus cross-contamination on the surface of porcine livers after storage in Euro meat containers in a German pig abattoir

Modernization of Control of Pathogenic Micro-Organisms in the Food-Chain Requires a Durable Role for Immunoaffinity-Based Detection Methodology—A Review

Das Hepatitis-E-Virus – ein zoonotisches Virus: Verbreitung, Übertragungswege und Bedeutung für die Lebensmittelsicherheit

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