High pressure extraction of bioactive diterpenes from the macroalgae <i>Bifurcaria bifurcata</i>: an efficient and environmentally friendly approach

Pais, Adriana; Pinto, Carlos A.; Ramos, Patrícia A. B.; Pinto, Ricardo J.B.; Rosa, Daniela; Duarte, Maria F.; Abreu, Helena; Rocha, Sı́lvia M.; Saraiva, Jorge A.; Silvestre, Armando J. D.; Santos, Sónia A.O.

doi:10.1039/c9ra06547d

Cited by 3 publications

(3 citation statements)

References 42 publications

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“…The lowest values (0.11 and 7.50 mg/ml respectively) was recorded for B. bifurcata, which indicated that the latter might more easily reach therapeutic concentration in further biological studies. These values were below that reported earlier by Pais and al. (2019), where extraction was performed from the same seaweed using dichloromethane solvent.…”

Section: Determination Of Mic and Mbccontrasting

confidence: 80%

Comparative Antibacterial Activity of Some Selected Seaweed Extracts from Agadir Coastal Regions in Morocco

Fayzi¹,

Askarne²,

Cherifi³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

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Section: Determination Of Mic and Mbccontrasting

confidence: 80%

Comparative Antibacterial Activity of Some Selected Seaweed Extracts from Agadir Coastal Regions in Morocco

Fayzi¹,

Askarne²,

Cherifi³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

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“…The antioxidant activity against DPPH of Pinus spp. bark extracts was approached as explained before [21,30] and adapted to the 96-well plate scale. Ascorbic acid was used as the antioxidant reference, being dissolved in methanol/water (50:50, v/v) at 1 mg mL −1 .…”

Section: Dpph Free Radical Scavenging Effectmentioning

confidence: 99%

Chemical Characterisation, Antioxidant and Antibacterial Activities of Pinus pinaster Ait. and Pinus pinea L. Bark Polar Extracts: Prospecting Forestry By-Products as Renewable Sources of Bioactive Compounds

et al. 2022

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Agroforestry by-products have gained rising attention in recent years as they represent inexpensive and abundant raw materials that are a source of added-value chemicals, e.g., for food and pharmaceutical applications, as well as for bioenergy generation. Pinus pinaster Ait. bark extracts are consumed worldwide for their cardiovascular benefits, whilst the health potential of Pinus pinea L. bark has not yet been deeply exploited. Therefore, this study highlights the chemical characterisation of Portuguese P. pinaster Ait. and P. pinea L. bark polar extracts, via ultra-high performance liquid chromatography-diode array detection-tandem mass spectrometry (UHPLC-DAD-MSn) analysis, and their antioxidant and antibacterial activities. Quinic acid, an A-type procyanidin dimer isomer, protocatechuic acid, and quercetin were identified for the first time as P. pinea L. bark components. Moreover, this bark demonstrated a higher total content of identified polar compounds than P. pinaster Ait. bark, with quinic acid being the most abundant compound identified. Regarding antioxidant activity, the pine bark polar extracts exhibited strong reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) radical scavenging effects compared to natural antioxidants. Moreover, the bactericidal actions of pine bark extracts were shown against Staphylococcus aureus and Escherichia coli at a 3.13–25 mg mL−1 range. Globally, these promising insights can boost the sustainable exploitation of P. pinea L. bark, as already occurs with P. pinaster Ait. bark, for the food and biomedical fields.

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“…HPE induces a significant pressure gradient between the cell's interior and exterior, inducing the structural deformation of the cell walls and membranes. This deformation increases the cells' permeability and, consequently, increases bioactive compound extraction into the solvent [18,19].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Chemical Composition and Biological Activities of Bog Bilberry (Vaccinium uliginosum L.) Leaf Extracts Obtained via Various Extraction Techniques

Ștefănescu,

Socaci,

Fărcaș

et al. 2024

Foods

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This investigation aimed to assess the chemical composition and biological activities of bog bilberry (Vaccinium uliginosum L.) leaves. Hydroethanolic extracts were obtained using four extraction techniques: one conventional (CE) and three alternative methods; ultrasound (UAE), microwave (MAE) and high-pressure (HPE) extractions. Spectrophotometric analysis was conducted to determine their chemical content, including the total phenolic content (TPC) and total flavonoid content (TFC). Furthermore, their antioxidative and antimicrobial properties were evaluated. HPLC (high performance liquid chromatography) analysis identified and quantified 17 phenolic compounds, with chlorogenic acid being the predominant compound, with the lowest level (37.36 ± 0.06 mg/g) for the bog bilberry leaf extract obtained by CE and the highest levels (e.g., HPE = 44.47 ± 0.08 mg/g) for the bog bilberry leaf extracts obtained by the alternative methods. Extracts obtained by HPE, UAE and MAE presented TPC values (135.75 ± 2.86 mg GAE/g; 130.52 ± 1.99 mg GAE/g; 119.23 ± 1.79 mg GAE/g) higher than those obtained by the CE method (113.07 ± 0.98 mg GAE/g). Regarding the TFC values, similar to TPC, the highest levels were registered in the extracts obtained by alternative methods (HPE = 43.16 ± 0.12 mg QE/g; MAE = 39.79 ± 0.41 mg QE/g and UAE = 33.89 ± 0.35 mg QE/g), while the CE extract registered the lowest level, 31.47 ± 0.28 mg QE/g. In the case of DPPH (1,1-diphenyl-2-picrylhydrazyl) antioxidant activity, the extracts from HPE, UAE and MAE exhibited the strongest radical scavenging capacities of 71.14%, 63.13% and 60.84%, respectively, whereas the CE extract registered only 55.37%. According to Microbiology Reader LogPhase 600 (BioTek), a common MIC value of 8.88 mg/mL was registered for all types of extracts against Staphylococcus aureus (Gram-positive bacteria) and Salmonella enterica (Gram-negative bacteria). Moreover, the alternative extraction methods (UAE, HPE) effectively inhibited the growth of Candida parapsilosis, in comparison to the lack of inhibition from the CE method. This study provides valuable insights into bog bilberry leaf extracts, reporting a comprehensive evaluation of their chemical composition and associated biological activities, with alternative extraction methods presenting greater potential for the recovery of phenolic compounds with increased biological activities than the conventional method.

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High pressure extraction of bioactive diterpenes from the macroalgae Bifurcaria bifurcata: an efficient and environmentally friendly approach

Abstract: Bioactive linear diterpenes were selectively extracted from the macroalga Bifurcaria bifurcata through optimized high-pressure extraction.

Cited by 3 publications

References 42 publications

Comparative Antibacterial Activity of Some Selected Seaweed Extracts from Agadir Coastal Regions in Morocco

Comparative Antibacterial Activity of Some Selected Seaweed Extracts from Agadir Coastal Regions in Morocco

Chemical Characterisation, Antioxidant and Antibacterial Activities of Pinus pinaster Ait. and Pinus pinea L. Bark Polar Extracts: Prospecting Forestry By-Products as Renewable Sources of Bioactive Compounds

Characterization of the Chemical Composition and Biological Activities of Bog Bilberry (Vaccinium uliginosum L.) Leaf Extracts Obtained via Various Extraction Techniques

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